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Photoregulating RNA Digestion Using Azobenzene Linked Dumbbell Antisense Oligodeoxynucleotides
Wu, Li1,2; He, Yujian1,2; Tang, Xinjing2
刊名BIOCONJUGATE CHEMISTRY
2015-06-01
DOI10.1021/acs.bioconjchem.5b00125
26期:6页:1070-1079
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
资助者National Natural Science Foundation of China ; National Basic Research Program of China ("973" Program) ; State Key Laboratory of Natural and Biomimetic Drugs ; National Natural Science Foundation of China ; National Basic Research Program of China ("973" Program) ; State Key Laboratory of Natural and Biomimetic Drugs
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]HUMAN LEUKEMIA-CELLS ; GENE-EXPRESSION ; REVERSIBLE PHOTOREGULATION ; CAGED SIRNAS ; DNA ; LIGHT ; OLIGONUCLEOTIDES ; HYBRIDIZATION ; ACTIVATION ; NUCLEOSIDE
英文摘要

Introduction of 4,4′-bis(hydroxymethyl)-azobenzene (azo) to dumbbell hairpin oligonucleotides at the loop position was able to reversibly control the stability of the whole hairpin structure via UV or visible light irradiation. Here, we designed and synthesized a series of azobenzene linked dumbbell antisense oligodeoxynucleotides (asODNs) containing two terminal hairpins that are composed of an asODN and a short inhibitory sense strand. Thermal melting studies of these azobenzene linked dumbbell asODNs indicated that efficient trans to cis photoisomerization of azobenzene moieties induced large difference in thermal stability (Delta T-m, = 12.1-21.3 degrees C). In addition, photomodulation of their RNA binding abilities and RNA digestion by RNase H was investigated. The trans-azobenzene linked asODNs with the optimized base pairs between asODN strands and inhibitory sense strands could only bind few percentage of the target RNA, while it was able to recover their binding to the target RNA and degrade it by RNase H after light irradiation. Upon optimization, it is promising to use these azobenzene linked asODNs for reversible spatial and temporal regulation of antisense activities based on both steric binding and RNA digestion by RNase H.

语种英语
所属项目编号21302008 ; 21422201 ; 21272263 ; 2012CB720600 ; K20140212 ; K20140204
资助者National Natural Science Foundation of China ; National Basic Research Program of China ("973" Program) ; State Key Laboratory of Natural and Biomimetic Drugs ; National Natural Science Foundation of China ; National Basic Research Program of China ("973" Program) ; State Key Laboratory of Natural and Biomimetic Drugs
WOS记录号WOS:000356754400014
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56381
专题北京大学药学院
作者单位1.Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 100049, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Wu, Li,He, Yujian,Tang, Xinjing. Photoregulating RNA Digestion Using Azobenzene Linked Dumbbell Antisense Oligodeoxynucleotides[J]. BIOCONJUGATE CHEMISTRY,2015,26(6):1070-1079.
APA Wu, Li,He, Yujian,&Tang, Xinjing.(2015).Photoregulating RNA Digestion Using Azobenzene Linked Dumbbell Antisense Oligodeoxynucleotides.BIOCONJUGATE CHEMISTRY,26(6),1070-1079.
MLA Wu, Li,et al."Photoregulating RNA Digestion Using Azobenzene Linked Dumbbell Antisense Oligodeoxynucleotides".BIOCONJUGATE CHEMISTRY 26.6(2015):1070-1079.
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