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学科主题: 药学
题名:
Photoregulating RNA Digestion Using Azobenzene Linked Dumbbell Antisense Oligodeoxynucleotides
作者: Wu, Li1,2; He, Yujian1,2; Tang, Xinjing2
刊名: BIOCONJUGATE CHEMISTRY
发表日期: 2015-06-01
DOI: 10.1021/acs.bioconjchem.5b00125
卷: 26, 期:6, 页:1070-1079
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: HUMAN LEUKEMIA-CELLS ; GENE-EXPRESSION ; REVERSIBLE PHOTOREGULATION ; CAGED SIRNAS ; DNA ; LIGHT ; OLIGONUCLEOTIDES ; HYBRIDIZATION ; ACTIVATION ; NUCLEOSIDE
英文摘要:

Introduction of 4,4′-bis(hydroxymethyl)-azobenzene (azo) to dumbbell hairpin oligonucleotides at the loop position was able to reversibly control the stability of the whole hairpin structure via UV or visible light irradiation. Here, we designed and synthesized a series of azobenzene linked dumbbell antisense oligodeoxynucleotides (asODNs) containing two terminal hairpins that are composed of an asODN and a short inhibitory sense strand. Thermal melting studies of these azobenzene linked dumbbell asODNs indicated that efficient trans to cis photoisomerization of azobenzene moieties induced large difference in thermal stability (Delta T-m, = 12.1-21.3 degrees C). In addition, photomodulation of their RNA binding abilities and RNA digestion by RNase H was investigated. The trans-azobenzene linked asODNs with the optimized base pairs between asODN strands and inhibitory sense strands could only bind few percentage of the target RNA, while it was able to recover their binding to the target RNA and degrade it by RNase H after light irradiation. Upon optimization, it is promising to use these azobenzene linked asODNs for reversible spatial and temporal regulation of antisense activities based on both steric binding and RNA digestion by RNase H.

语种: 英语
所属项目编号: 21302008 ; 21422201 ; 21272263 ; 2012CB720600 ; K20140212 ; K20140204
项目资助者: National Natural Science Foundation of China ; National Basic Research Program of China ("973" Program) ; State Key Laboratory of Natural and Biomimetic Drugs
WOS记录号: WOS:000356754400014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56381
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 100049, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Wu, Li,He, Yujian,Tang, Xinjing. Photoregulating RNA Digestion Using Azobenzene Linked Dumbbell Antisense Oligodeoxynucleotides[J]. BIOCONJUGATE CHEMISTRY,2015,26(6):1070-1079.
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