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学科主题: 临床医学
题名:
Up-regulation of urinary-type plasminogen activator correlates with high-risk papillary thyroid carcinoma with BRAF(V600E) mutation and its possible molecular mechanism
作者: Wakasa, Tomoko1; Li, Yaqiong2; Bai, Yanhua3; Liu, Zhiyan4; Ozaki, Takashi5; Mori, Ichiro6; Miyauchi, Akira7; Kakudo, Kennichi8; Nakamura, Misa9
关键词: Urinary-type plasminogen activator ; Papillary thyroid cancer ; BRAF ; ERK1/2 ; MEK inhibitor
刊名: PATHOLOGY RESEARCH AND PRACTICE
发表日期: 2014
DOI: 10.1016/j.prp.2014.06.025
卷: 210, 期:11, 页:733-738
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pathology
研究领域[WOS]: Pathology
关键词[WOS]: BRAF V600E MUTATION ; CANCER-CELLS ; COMMON TYPE ; EXPRESSION ; KINASE ; GENE ; SUBCLASSIFICATION ; IDENTIFICATION ; PATHWAY ; SYSTEM
英文摘要:

The aim of the present study is to investigate the relationship between urinary-type plasminogen activator (uPA) expression and clinicopathological features in papillary thyroid carcinoma (FTC) and to determine the signal transduction of PTC cells in vitro.

PTC tissues from 42 patients were analyzed for the expression of uPA and the BRAF(V600E) mutation. BCPAP, a PTC cell line harboring the BRAF(V600E) mutation, was used to study MAPK signaling. PCR and direct sequencing were applied to analyze BRAF(V600E) mutation status. uPA mRNA expression was measured using a quantitative RT-PCR method, and uPA protein was localized using an immunohistochemical method. The ERK protein status was detected by Western blot analysis.

uPA gene expression was significantly increased in PTC tissues as compared to the corresponding non-tumor tissues. Furthermore, the up-regulation of uPA mRNAs was correlated with high-risk clinicopathological features, including extrathyroid invasion, loss of cellular polarity/cohesiveness, and the BRAF(V600E) mutation. Marked dephosphorylation of ERK1/2 and down-regulation of uPA expression were detected when BCPAP was treated with a MEK inhibitor, U0126.

MEK inhibitors might be a potential treatment strategy for aggressive PTC with BRAF(V600E) through inhibition of uPA expression. (C) 2014 Elsevier GmbH. All rights reserved.

语种: 英语
WOS记录号: WOS:000344836400005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56395
Appears in Collections:北京大学临床肿瘤学院_病理科_期刊论文

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作者单位: 1.Social Insurance Kinan Hosp, Wakayama 646858, Japan
2.Kuma Hosp, Dept Surg, Chuo Ku, Kobe, Hyogo 6500011, Japan
3.Tokiwa Univ, Dept Med Technol, Nagata Ku, Kobe, Hyogo 6530838, Japan
4.Taishan Med Univ, Dept Pathol, Tai An 271000, Shandong, Peoples R China
5.Kinki Univ, Nara Hosp, Sch Med, Dept Pathol & Lab Med, Nara 6300293, Japan
6.Peking Univ, Canc Hosp & Inst, Dept Pathol, Key Lab Carcinogenesis & Translat Res Minist Educ, Beijing 100871, Peoples R China
7.Shandong Univ, Sch Med, Dept Pathol & Pathophysiol, Jinan 250012, Shandong, Peoples R China
8.Int Univ Hlth & Welf, Mita Hosp, Dept Pathol, Minato Ku, Tokyo 1088329, Japan
9.Osaka Kawasaki Rehabil Univ, Dept Rehabil, Kaizuka City, Osaka 5970104, Japan

Recommended Citation:
Wakasa, Tomoko,Li, Yaqiong,Bai, Yanhua,et al. Up-regulation of urinary-type plasminogen activator correlates with high-risk papillary thyroid carcinoma with BRAF(V600E) mutation and its possible molecular mechanism[J]. PATHOLOGY RESEARCH AND PRACTICE,2014,210(11):733-738.
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