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学科主题: 基础医学
题名:
A protein in pig spleen similar to immune suppressive protein of stress
作者: Fu, HY; Zhang, J; Ding, GF; Fan, SG
关键词: immune function ; lymphocytes ; stress ; spleen
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2003-06-01
卷: 24, 期:6, 页:593-598
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: LYMPHOCYTE-PROLIFERATION ; RESTRAINT STRESS ; AGRIN ; MICE
英文摘要:

AIM: To purify a protein in pig spleens, which was similar to immune suppressive protein of stress (ISPS), and characterize its properties and functions. METHODS: 1) Pig spleen was extracted in dilute hydrochloric acid. 2) The extract was ultra-filtrated for having high molecular weight proteins (M(r)>30 000). 3) The filtrates were purified with FPLC affinity chromatography. 4) The elute from FPLC was used for T-lymphocyte proliferation and ELISA test. 5) Lastly, SDS-PAGE was used to determine the molecular weight and purity of the final product. RESULTS: A protein purified from pig spleen (the pig ISPS homologue) inhibited concanavalin A (Con A)-induced mouse lymphocyte proliferation. The molecular weight of this protein was about M, 190 000. It has a stronger selectivity against T-lymphocyte line such as Jurkat cell line and mastocyte line (P815) and has a weaker inhibitory activity on macrophage line (U937). CONCLUSION: A protein similar to rat/mouse ISPS was found in pig spleen. This may provide an opportunity to study its roles in tumors and autoimmune diseases.

语种: 英语
WOS记录号: WOS:000183575100017
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56403
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Fu, HY,Zhang, J,Ding, GF,et al. A protein in pig spleen similar to immune suppressive protein of stress[J]. ACTA PHARMACOLOGICA SINICA,2003,24(6):593-598.
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