北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 免疫学系  > 期刊论文
学科主题: 基础医学
题名:
A protein in pig spleen similar to immune suppressive protein of stress
作者: Fu, HY; Zhang, J; Ding, GF; Fan, SG
关键词: immune function ; lymphocytes ; stress ; spleen
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2003-06-01
卷: 24, 期:6, 页:593-598
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: LYMPHOCYTE-PROLIFERATION ; RESTRAINT STRESS ; AGRIN ; MICE
英文摘要:

AIM: To purify a protein in pig spleens, which was similar to immune suppressive protein of stress (ISPS), and characterize its properties and functions. METHODS: 1) Pig spleen was extracted in dilute hydrochloric acid. 2) The extract was ultra-filtrated for having high molecular weight proteins (M(r)>30 000). 3) The filtrates were purified with FPLC affinity chromatography. 4) The elute from FPLC was used for T-lymphocyte proliferation and ELISA test. 5) Lastly, SDS-PAGE was used to determine the molecular weight and purity of the final product. RESULTS: A protein purified from pig spleen (the pig ISPS homologue) inhibited concanavalin A (Con A)-induced mouse lymphocyte proliferation. The molecular weight of this protein was about M, 190 000. It has a stronger selectivity against T-lymphocyte line such as Jurkat cell line and mastocyte line (P815) and has a weaker inhibitory activity on macrophage line (U937). CONCLUSION: A protein similar to rat/mouse ISPS was found in pig spleen. This may provide an opportunity to study its roles in tumors and autoimmune diseases.

语种: 英语
WOS记录号: WOS:000183575100017
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56403
Appears in Collections:基础医学院_免疫学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
593.pdf(410KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Fu, HY,Zhang, J,Ding, GF,et al. A protein in pig spleen similar to immune suppressive protein of stress[J]. ACTA PHARMACOLOGICA SINICA,2003,24(6):593-598.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Fu, HY]'s Articles
[Zhang, J]'s Articles
[Ding, GF]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Fu, HY]‘s Articles
[Zhang, J]‘s Articles
[Ding, GF]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace