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学科主题: 临床医学
题名:
Characterizing the interaction between oridonin and bovine serum albumin by a hybrid spectroscopic approach
作者: Wang, Zhen1; Chen, Junhui2; Wang, Shaobin3; Chen, Zhanguang1
关键词: Oridonin ; Bovine serum albumin ; Multispectroscopic techniques ; Noncovalent binding
刊名: JOURNAL OF LUMINESCENCE
发表日期: 2013-02-01
DOI: 10.1016/j.jlumin.2012.06.035
卷: 134, 页:863-869
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Optics
研究领域[WOS]: Optics
关键词[WOS]: RESONANCE LIGHT-SCATTERING ; NUCLEIC-ACIDS ; ANTICANCER DRUG ; IN-VITRO ; SENSITIVE ASSAY ; BINDING ; DNA ; FLUORESCENCE ; DERIVATIVES ; SYSTEM
英文摘要:

Oridonin is an effective anticancer drug which has high potency and low systemic toxicity. In this study, the interaction between oridonin and bovine serum albumin (BSA) was investigated by several spectroscopic approaches for the first time. The binding characteristics of oridonin and BSA were determined by fluorescence emission spectra and resonance light scattering spectra. It is showed that the oridonin quenches the fluorescence of BSA and the static quenching constant K-SV is 1.30 x 10(4) L mol(-1) at 298 K. Moreover, oridonin and BSA form a 1:1 complex with a binding constant of 0.62 x 104 L mol(-1). On the other hand, the thermodynamic parameters indicate that the binding process was a spontaneous molecular interaction procedure, in which hydrophobic forces played a major role. The structure analysis indicates that oridonin binding results in an increased hydrophobicity around the tryptophan residues of BSA. Additionally, as shown by the UV-vis absorption, synchronous fluorescence and three-dimensional fluorescence results, oridonin could lead to conformational and some microenvironmental changes of BSA. The work provides accurate and full basic data for clarifying the binding mechanism of oridonin with BSA in vitro and is helpful for understanding its effect on protein function during its transportation and distribution in blood. (c) 2012 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 9151022501000019
项目资助者: Natural Science Foundation of Guangdong Province
WOS记录号: WOS:000313393300136
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56409
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Fourth Peoples Hosp Shenzhen, Shenzhen 518033, Peoples R China
3.Peking Univ, Shenzhen Hosp, Intervent Oncol & Minimally Invas Therapies Dept, Shenzhen 518036, Peoples R China

Recommended Citation:
Wang, Zhen,Chen, Junhui,Wang, Shaobin,et al. Characterizing the interaction between oridonin and bovine serum albumin by a hybrid spectroscopic approach[J]. JOURNAL OF LUMINESCENCE,2013,134:863-869.
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