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学科主题: 基础医学
题名:
Structure-function correlation of human programmed cell death 5 protein
作者: Yao, Hongwei2; Xu, Lanjun1; Feng, Yingang2; Liu, Dongsheng2; Chen, Yingyu1; Wang, Jinfeng2
关键词: PDCD5 ; NMR ; Structure-function relationship ; Cell translocation ; Fragments of PDCD5
刊名: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2009-06-15
DOI: 10.1016/j.abb.2009.03.018
卷: 486, 期:2, 页:141-149
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: GENE-EXPRESSION PROFILES ; CELL-DEATH-5 PROTEIN ; TUMOR-CELLS ; NMR ; APOPTOSIS ; TFAR19 ; PHOSPHATIDYLSERINE ; RESTRAINTS ; ALIGNMENT ; DYNAMICS
英文摘要:

Human programmed cell death 5 (PDCD5) is a translocatory protein playing ail important role in the apoptotic process of cells. Although there are accumulated data about PDCD5 function, the correlation of the structure with the function of PDCD5 has not been investigated. Here, we report the studies of structure-function relationship of PDCD5 by multidimensional NMR methods and by FACScan flow cytometer and fluorescence microscope. The 3D structure of intact PDCD5 and the internal motions of PDCD5 have been determined. PDCD5 has a compact core structure of low flexibility with two mobile alpha-helices at N-terminal region and a flexible Unstructured C-terminal region. The flow cytometry and internalization measurements of different PDCD5 fragments indicate that the charged residues are crucial for the ability of apoptosis-promoting and cell translocation of the protein. Combined analyses reveal a fact that the regions that seem to be most involved in the function also are more flexible in PDCD5. (C) 2009 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: NNSFC 30170201 ; 2002BA711A13 ; KSCX1-SW-17
项目资助者: National Natural Science Foundation of China ; 863 Special Program of China ; Chinese Academy of Sciences
WOS记录号: WOS:000266940300007
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56411
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Lab Med Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

Recommended Citation:
Yao, Hongwei,Xu, Lanjun,Feng, Yingang,et al. Structure-function correlation of human programmed cell death 5 protein[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2009,486(2):141-149.
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