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学科主题基础医学
Structure-function correlation of human programmed cell death 5 protein
Yao, Hongwei2; Xu, Lanjun1; Feng, Yingang2; Liu, Dongsheng2; Chen, Yingyu1; Wang, Jinfeng2
关键词PDCD5 NMR Structure-function relationship Cell translocation Fragments of PDCD5
刊名ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
2009-06-15
DOI10.1016/j.abb.2009.03.018
486期:2页:141-149
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]GENE-EXPRESSION PROFILES ; CELL-DEATH-5 PROTEIN ; TUMOR-CELLS ; NMR ; APOPTOSIS ; TFAR19 ; PHOSPHATIDYLSERINE ; RESTRAINTS ; ALIGNMENT ; DYNAMICS
英文摘要

Human programmed cell death 5 (PDCD5) is a translocatory protein playing ail important role in the apoptotic process of cells. Although there are accumulated data about PDCD5 function, the correlation of the structure with the function of PDCD5 has not been investigated. Here, we report the studies of structure-function relationship of PDCD5 by multidimensional NMR methods and by FACScan flow cytometer and fluorescence microscope. The 3D structure of intact PDCD5 and the internal motions of PDCD5 have been determined. PDCD5 has a compact core structure of low flexibility with two mobile alpha-helices at N-terminal region and a flexible Unstructured C-terminal region. The flow cytometry and internalization measurements of different PDCD5 fragments indicate that the charged residues are crucial for the ability of apoptosis-promoting and cell translocation of the protein. Combined analyses reveal a fact that the regions that seem to be most involved in the function also are more flexible in PDCD5. (C) 2009 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000266940300007
项目编号NNSFC 30170201 ; 2002BA711A13 ; KSCX1-SW-17
资助机构National Natural Science Foundation of China ; 863 Special Program of China ; Chinese Academy of Sciences
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56411
专题北京大学基础医学院_免疫学系
北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Lab Med Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
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Yao, Hongwei,Xu, Lanjun,Feng, Yingang,et al. Structure-function correlation of human programmed cell death 5 protein[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2009,486(2):141-149.
APA Yao, Hongwei,Xu, Lanjun,Feng, Yingang,Liu, Dongsheng,Chen, Yingyu,&Wang, Jinfeng.(2009).Structure-function correlation of human programmed cell death 5 protein.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,486(2),141-149.
MLA Yao, Hongwei,et al."Structure-function correlation of human programmed cell death 5 protein".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 486.2(2009):141-149.
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