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学科主题临床医学
Hepatitis B Virus Inhibits Apoptosis of Hepatoma Cells by Sponging the MicroRNA 15a/16 Cluster
Liu, Ningning1,2; Zhang, Jinfang1; Jiao, Tong1,2; Li, Zhiwei3; Peng, Jirun4; Cui, Zhuqingqing4; Ye, Xin1
刊名JOURNAL OF VIROLOGY
2013-12-01
DOI10.1128/JVI.02130-13
87期:24页:13370-13378
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Virology
研究领域[WOS]Virology
关键词[WOS]HEPATOCELLULAR-CARCINOMA ; X-PROTEIN ; EXPRESSION ; HEPATOCYTES ; BCL-2 ; HEPATOCARCINOGENESIS ; BIOGENESIS ; PATHWAYS ; GENE
英文摘要

Hepatitis B virus (HBV) causes chronic hepatitis in hundreds of millions of people worldwide, which can eventually lead to hepatocellular carcinoma (HCC). The molecular mechanisms underlying HBV persistence are not well understood. In this study, we found that HBV inhibited the chemotherapy drug etoposide-induced apoptosis of hepatoma cells. Further analysis revealed that HBV mRNAs possess a microRNA 15a/16 (miR-15a/16)-complementary site (HBV nucleotides [nt] 1362 to 1383) that acts as a sponge to bind and sequester endogenous miR-15a/16. Consequently, Bcl-2, known as the target of miR-15a/16, was upregulated in HBV-infected cells. The data from HBV-transgenic mice further confirmed that HBV transcripts cause the reduction of miR-15a/16 and increase of Bcl-2. More importantly, we examined the levels of HBV transcripts and miR-15a/16 in HBV-infected HCC from patients and found that the amount of HBV mRNA and the level of miR-15a/16 were negatively correlated. Consistently, the level of Bcl-2 mRNA was upregulated in HBV-infected patients. In conclusion, we identified a novel HBV mRNA-miR-15a/16-Bcl-2 regulatory pathway that is involved in inhibiting etoposide-induced apoptosis of hepatoma cells, which may contribute to facilitating chronic HBV infection and hepatoma development.

语种英语
WOS记录号WOS:000327443300027
项目编号2012CB519003 ; 2013ZX10004611 ; 2011CB504705 ; 2012ZX10004501-001-003 ; 81272272 ; 81021003 ; KSCX2-EW-J-6
资助机构Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences
引用统计
被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56450
专题北京大学第二临床医学院
作者单位1.302 Hosp PLA, Beijing, Peoples R China
2.Peking Univ, Peoples Hosp, Beijing 100871, Peoples R China
3.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Ctr Mol Immunol, Beijing, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
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GB/T 7714
Liu, Ningning,Zhang, Jinfang,Jiao, Tong,et al. Hepatitis B Virus Inhibits Apoptosis of Hepatoma Cells by Sponging the MicroRNA 15a/16 Cluster[J]. JOURNAL OF VIROLOGY,2013,87(24):13370-13378.
APA Liu, Ningning.,Zhang, Jinfang.,Jiao, Tong.,Li, Zhiwei.,Peng, Jirun.,...&Ye, Xin.(2013).Hepatitis B Virus Inhibits Apoptosis of Hepatoma Cells by Sponging the MicroRNA 15a/16 Cluster.JOURNAL OF VIROLOGY,87(24),13370-13378.
MLA Liu, Ningning,et al."Hepatitis B Virus Inhibits Apoptosis of Hepatoma Cells by Sponging the MicroRNA 15a/16 Cluster".JOURNAL OF VIROLOGY 87.24(2013):13370-13378.
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