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学科主题: 临床医学
题名:
Hepatitis B Virus Inhibits Apoptosis of Hepatoma Cells by Sponging the MicroRNA 15a/16 Cluster
作者: Liu, Ningning1,2; Zhang, Jinfang1; Jiao, Tong1,2; Li, Zhiwei3; Peng, Jirun4; Cui, Zhuqingqing4; Ye, Xin1
刊名: JOURNAL OF VIROLOGY
发表日期: 2013-12-01
DOI: 10.1128/JVI.02130-13
卷: 87, 期:24, 页:13370-13378
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: HEPATOCELLULAR-CARCINOMA ; X-PROTEIN ; EXPRESSION ; HEPATOCYTES ; BCL-2 ; HEPATOCARCINOGENESIS ; BIOGENESIS ; PATHWAYS ; GENE
英文摘要:

Hepatitis B virus (HBV) causes chronic hepatitis in hundreds of millions of people worldwide, which can eventually lead to hepatocellular carcinoma (HCC). The molecular mechanisms underlying HBV persistence are not well understood. In this study, we found that HBV inhibited the chemotherapy drug etoposide-induced apoptosis of hepatoma cells. Further analysis revealed that HBV mRNAs possess a microRNA 15a/16 (miR-15a/16)-complementary site (HBV nucleotides [nt] 1362 to 1383) that acts as a sponge to bind and sequester endogenous miR-15a/16. Consequently, Bcl-2, known as the target of miR-15a/16, was upregulated in HBV-infected cells. The data from HBV-transgenic mice further confirmed that HBV transcripts cause the reduction of miR-15a/16 and increase of Bcl-2. More importantly, we examined the levels of HBV transcripts and miR-15a/16 in HBV-infected HCC from patients and found that the amount of HBV mRNA and the level of miR-15a/16 were negatively correlated. Consistently, the level of Bcl-2 mRNA was upregulated in HBV-infected patients. In conclusion, we identified a novel HBV mRNA-miR-15a/16-Bcl-2 regulatory pathway that is involved in inhibiting etoposide-induced apoptosis of hepatoma cells, which may contribute to facilitating chronic HBV infection and hepatoma development.

语种: 英语
所属项目编号: 2012CB519003 ; 2013ZX10004611 ; 2011CB504705 ; 2012ZX10004501-001-003 ; 81272272 ; 81021003 ; KSCX2-EW-J-6
项目资助者: Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences
WOS记录号: WOS:000327443300027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56450
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.302 Hosp PLA, Beijing, Peoples R China
2.Peking Univ, Peoples Hosp, Beijing 100871, Peoples R China
3.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Ctr Mol Immunol, Beijing, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

Recommended Citation:
Liu, Ningning,Zhang, Jinfang,Jiao, Tong,et al. Hepatitis B Virus Inhibits Apoptosis of Hepatoma Cells by Sponging the MicroRNA 15a/16 Cluster[J]. JOURNAL OF VIROLOGY,2013,87(24):13370-13378.
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