北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学深圳医院  > 神经内科  > 期刊论文
学科主题: 临床医学
题名:
Activation of Wnt/beta-catenin Pathway by Exogenous Wnt1 Protects SH-SY5Y Cells Against 6-Hydroxydopamine Toxicity
作者: Wei, Lei1,2; Sun, Congcong1; Lei, Ming1; Li, Guofei3; Yi, Li4; Luo, Feifei5; Li, Yi6; Ding, Li7; Liu, Zhuolin1; Li, Shaomin8; Xu, Pingyi1
关键词: Wnt1 ; Oxidative stress ; Endoplasmic reticulum (ER) stress ; Parkinson&prime ; s disease
刊名: JOURNAL OF MOLECULAR NEUROSCIENCE
发表日期: 2013
DOI: 10.1007/s12031-012-9900-8
卷: 49, 期:1, 页:105-115
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]: Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]: ENDOPLASMIC-RETICULUM STRESS ; GLYCOGEN-SYNTHASE KINASE-3-BETA ; PARKINSONS-DISEASE ; SIGNALING PATHWAYS ; OXIDATIVE STRESS ; DEATH ; APOPTOSIS ; SURVIVAL ; NEURONS ; NEURODEGENERATION
英文摘要:

Wnt1, initially described as a modulator of embryonic development, has recently been discovered to exert cytoprotective effects in cellular models of several diseases, including Parkinson′s disease (PD). We, therefore, examined the neuroprotective effects of exogenous Wnt1 on dopaminergic SH-SY5Y cells treated with 6-hydroxydopamine (6-OHDA). Here, we show that 10-500 mu M 6-OHDA treatment decreased cell viability and increased lactate dehydrogenase (LDH) leakage. SH-SY5Y cells treated with 100 mu M 6-OHDA for 24 h showed reduced Wnt/beta-catenin activity, decreased mitochondrial transmembrane potential, elevated levels of reactive oxidative species (ROS) and phosphatidylserine (PS) extraversion, increased levels of Chop and Bip/GRP78 and reduced level of p-Akt (Ser473). In contrast, exogenous Wnt1 attenuated 6-OHDA-induced changes. These results suggest that activation of the Wnt/beta-catenin pathway by exogenous Wnt1 protects against 6-OHDA-induced changes by restoring mitochondria and endoplasmic reticulum (ER) function.

语种: 英语
所属项目编号: 2007AA02Z460 ; 2010AB973Z531 ; 81071032 ; 81271428 ; 2012J4300061 ; 20100171110056 ; ZDXM080214
项目资助者: Ministry of Science and Technology of the People&prime ; s Republic of China ; Nature Science Foundation of China ; Guangzhou Foundation for Scientific and Technological Project, China ; Specialized Research Fund for the Doctoral Program of Higher Education of China ; Hainan Provincial Science and Technology Department
WOS记录号: WOS:000312881900012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56452
Appears in Collections:北京大学深圳医院_神经内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Sun Yat Sen Univ, Dept Neurol, Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China
2.Sun Yat Sen Univ, Dept Neurol, Affiliated Hosp 3, Guangzhou 510630, Guangdong, Peoples R China
3.Henan Univ Hosp Huaihe, Dept Neurol, Kaifeng 37500, Henan, Peoples R China
4.Beijing Univ, Dept Neurol, Shenzhen Hosp, Shenzhen 518036, Peoples R China
5.Chongqing Med Univ, Dept Neurol, Affiliated Hosp Chengdu 2, Peoples Hosp Chengdu 3, Chengdu 610031, Peoples R China
6.Jinan Univ, Dept Neurol, Guangzhou Red Cross Hosp, Affiliated Hosp 4, Guangzhou 510220, Guangdong, Peoples R China
7.Sun Yat Sen Univ, Dept Pathol, Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China
8.Harvard Univ, Sch Med, Dept Neurol, Brigham & Womens Hosp, Boston, MA 02115 USA

Recommended Citation:
Wei, Lei,Sun, Congcong,Lei, Ming,et al. Activation of Wnt/beta-catenin Pathway by Exogenous Wnt1 Protects SH-SY5Y Cells Against 6-Hydroxydopamine Toxicity[J]. JOURNAL OF MOLECULAR NEUROSCIENCE,2013,49(1):105-115.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wei, Lei]'s Articles
[Sun, Congcong]'s Articles
[Lei, Ming]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wei, Lei]‘s Articles
[Sun, Congcong]‘s Articles
[Lei, Ming]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace