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学科主题: 临床医学
题名:
CCR1 and CCR5 expression on inflammatory cells is related to cigarette smoking and chronic obstructive pulmonary disease severity
作者: Wang Fei; He Bei
关键词: pulmonary disease, chronic obstructive ; smoke ; inflammation ; receptors, CCR1 ; receptors, CCR5
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2012-12-05
DOI: 10.3760/cma.j.issn.0366-6999.2012.23.021
卷: 125, 期:23, 页:4277-4282
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: INDUCED SPUTUM ; AIRWAY-OBSTRUCTION ; COPD ; RECEPTOR ; LYMPHOCYTES ; LAVAGE ; ASTHMA ; MICE
英文摘要:

Background Chronic obstructive pulmonary disease (COPD) is a progressive disease associated with a cellular inflammatory response mostly concerned with cigarette smoking. Chemokine receptors CCR1/5 play an important role in the inflammatory cells recruitment in the lung of COPD patients. The aim of this study was to determine the impact of cigarette smoking on the expression of CCR1/5 on inflammatory cells in induced sputum, and the relationship between the receptors expression and COPD severity.

Methods Differential cells in induced sputum were counted and the optical densities of CCR1 and CCR5 on inflammatory cells in induced sputum from COPD patients (n=29), healthy smokers (n=11), and nonsmokers (n=6) were measured using immunocytochemistry. Concentrations of CCL3, the ligand of CCR1/5, in supernatant of induced sputum were detected by enzyme-linked immunosorbent assay.

Results The expressions of CCR1 and CCR5 on inflammatory cells in healthy smokers were significantly higher than those in nonsmokers, and the expression of CCR1 in patients with COPD was significantly increased when compared with nonsmokers but not healthy smokers. The expressions of CCR1 and CCR5 on inflammatory cells in severe and very severe COPD patients were higher compared with mild and moderate COPD patients. CCL3 level was positively correlated with the total cell counts in induced sputum and smoking history, and negatively correlated with percentage of predicted FEV1.

Conclusions Cigarette smoking could increase the expression of CCR1 on the inflammatory cells. Both CCR1 and CCR5 expressions on the inflammatory cells in induced sputum could be associated with COPD severity. Chin Med J 2012;125(23):4277-4282

语种: 英语
所属项目编号: 30370608 ; 20050001143
项目资助者: National Natural Science Foundation of China ; Ph.D. Programs Foundation of Ministry of Education of China
WOS记录号: WOS:000312748200021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56457
Appears in Collections:北京大学第三临床医学院_呼吸内科_期刊论文

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作者单位: Peking Univ Third Hosp, Dept Resp Dis, Beijing 100191, Peoples R China

Recommended Citation:
Wang Fei,He Bei. CCR1 and CCR5 expression on inflammatory cells is related to cigarette smoking and chronic obstructive pulmonary disease severity[J]. CHINESE MEDICAL JOURNAL,2012,125(23):4277-4282.
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