IR@PKUHSC  > 北京大学口腔医学院  > 儿童口腔科
学科主题口腔医学
Msx1 regulates proliferation and differentiation of mouse dental mesenchymal cells in culture
Feng, Xiao-yu; Zhao, Yu-ming; Wang, Wen-jun; Ge, Li-hong
关键词Bmp2 Development Sirna Tooth Germ Bmp4
刊名EUROPEAN JOURNAL OF ORAL SCIENCES
2013-10-01
DOI10.1111/eos.12078
121期:5页:412-420
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]CRANIAL NEURAL CREST ; TOOTH DEVELOPMENT ; EXPRESSION PATTERNS ; HOMEOBOX GENES ; BONE-FORMATION ; CLEFT-PALATE ; MORPHOGENESIS ; BMP4 ; ODONTOGENESIS ; INHIBITION
英文摘要

The homeobox, msh-like 1 (MSX1) protein is essential for cell proliferation and differentiation. Tooth germ development of Msx1 knockout mouse is arrested at the bud stage, impeding an understanding of its role beyond this stage of tooth development. The aims of this study were to investigate the potential role of MSX1 in the regulation of proliferation and differentiation of dental mesenchymal cells in culture, and to preliminarily explore its underlying mechanism of action. Tooth germs were isolated from embryonic day (E)15.5 mice. The mesenchyme was separated and digested into a single-cell suspension, and then cultured in vitro. Isolated dental mesenchymal cells were transfected with MSX1 small interfering RNA, and the effects on cell proliferation, cell cycle distribution, and the expression of bone morphogenetic protein 2 (Bmp2) and bone morphogenetic protein 4 (Bmp4) were studied. We also compared the expression levels of alkaline phosphatase (Alp), type I collagen (Col1A), osteocalcin (Ocn), runt-related transcription factor 2 (Runx2), dentin sialophosphoprotein (Dspp) and dentin matrix protein 1 (Dmp1), and mineralized nodule formation, between control and MSX1 siRNA-transfected groups after the induction of odontoblast differentiation. Knockdown of Msx1 expression was associated with decreased cell proliferation, prolonged time in the S phase of the cell cycle, enhanced odontoblast differentiation, and elevated Bmp2 and Bmp4 expression. We conclude that MSX1 may promote proliferation and prevent the differentiation of dental mesenchymal cells by the inhibition of Bmp2 and Bmp4 expression.

语种英语
所属项目编号81170928 ; 81141011
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000324315700004
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56489
Collection北京大学口腔医学院_儿童口腔科
作者单位Peking Univ, Dept Pediat Dent, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Feng, Xiao-yu,Zhao, Yu-ming,Wang, Wen-jun,et al. Msx1 regulates proliferation and differentiation of mouse dental mesenchymal cells in culture[J]. EUROPEAN JOURNAL OF ORAL SCIENCES,2013,121(5):412-420.
APA Feng, Xiao-yu,Zhao, Yu-ming,Wang, Wen-jun,&Ge, Li-hong.(2013).Msx1 regulates proliferation and differentiation of mouse dental mesenchymal cells in culture.EUROPEAN JOURNAL OF ORAL SCIENCES,121(5),412-420.
MLA Feng, Xiao-yu,et al."Msx1 regulates proliferation and differentiation of mouse dental mesenchymal cells in culture".EUROPEAN JOURNAL OF ORAL SCIENCES 121.5(2013):412-420.
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