IR@PKUHSC  > 北京大学深圳医院  > 泌尿外科
学科主题临床医学
High expression of KPNA2 defines poor prognosis in patients with upper tract urothelial carcinoma treated with radical nephroureterectomy
Shi, Bentao1,2; Su, Boxing2,3; Fang, Dong2,3; Tang, Yuan2,3; Xiong, Gengyan2,3; Guo, Zhongqiang2,3; He, Qun2,4,5; Yang, Xinyu2,4,5; Zhao, Wei6; Guo, Yinglu2,3; Li, Xuesong2,3; Zhou, Liqun2,3
关键词Kpna2 Upper Tract Urothelial Carcinoma Prognosis Bladder Recurrence
刊名BMC CANCER
2015-05-09
DOI10.1186/s12885-015-1369-8
15
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]UPPER URINARY-TRACT ; TRANSITIONAL-CELL CARCINOMA ; NUCLEAR-TRANSPORT RECEPTOR ; INTRAVESICAL RECURRENCE ; KARYOPHERIN ALPHA-2 ; PREDICTIVE FACTORS ; SUBUNIT ALPHA-2 ; PROTEIN IMPORT ; BREAST-CANCER ; LUNG-CANCER
英文摘要

Background: To analyze the expression of karyopherin alpha 2 (KPNA2) in upper tract urothelial carcinoma (UTUC) and to investigate whether the KPNA2 expression provides additional prognostic information following radical nephroureterectomy (RNU).

Methods: A tissue microarray (TMA) containing samples from 176 patients with UTUC who underwent RNU at our institute was analyzed for KPNA2 expression using immunohistochemistry. KPNA2 expression in normal urothelial cell line and urothelial carcinoma cell lines was evaluated by western blot analysis. Using RNA interference in vitro, the effects of KPNA2 inhibition on cellular viability, migration and apoptosis were determined.

Results: KPNA2 expression was significantly upregulated in the UTUC samples compared with the adjacent normal urothelial tissues. High KPNA2 immunoreactivity was identified as a predictor of bladder recurrence (hazard ratio [HR]: 2.017, 95% CI 1.13-3.61, p = 0.018), poor disease-free survival (DFS, HR: 2.754, 95% CI 1.68-4.51, p = 0.001) and poor overall survival (OS, HR: 4.480, 95% CI 1.84-10.89, p = 0.001) for patients with UTUC after RNU. Furthermore, high KPNA2 immunoreactivity was independent of the conventional predictive factors in a multivariate analysis. Additional in vitro experiments revealed that KPNA2 expression was higher in urothelial carcinoma cell lines than in normal urothelial cell line. KPNA2 inhibition with a specific siRNA decreased cell viability and migration and increased apoptosis in urothelial carcinoma cell lines.

Conclusions: KPNA2 is a novel independent prognostic marker for bladder recurrence, DFS and OS of UTUC patients who have undergone RNU. Moreover, these data suggest that KPNA2 may be a promising therapeutic target for UTUC.

语种英语
WOS记录号WOS:000354429700001
项目编号7122183 ; BMU20120318 ; BMU20120318 ; 81172419 ; 81372746 ; A2014653 ; 201302052
资助机构Beijing Natural Science Foundation ; Peking University Health Science Center ; National Taiwan University, College of Medicine ; National Natural Science Foundation ; Medical Scientific Research Foundation of Guangdong Province, China ; Science and Technology Project in Shenzhen
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56499
专题北京大学深圳医院_泌尿外科
北京大学第一临床医学院_泌尿外科
北京大学第一临床医学院_北京大学泌尿外科研究所
作者单位1.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
3.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen 518036, Guangdong, Peoples R China
4.Peking Univ, Beijing & Inst Urol, Natl Urol Canc Ctr, Beijing 100034, Peoples R China
5.Peking Univ, Hosp 1, Dept Urol Pathol, Beijing 100034, Peoples R China
6.Peking Univ, Dept Cell Biol, Sch Oncol, Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China
推荐引用方式
GB/T 7714
Shi, Bentao,Su, Boxing,Fang, Dong,et al. High expression of KPNA2 defines poor prognosis in patients with upper tract urothelial carcinoma treated with radical nephroureterectomy[J]. BMC CANCER,2015,15.
APA Shi, Bentao.,Su, Boxing.,Fang, Dong.,Tang, Yuan.,Xiong, Gengyan.,...&Zhou, Liqun.(2015).High expression of KPNA2 defines poor prognosis in patients with upper tract urothelial carcinoma treated with radical nephroureterectomy.BMC CANCER,15.
MLA Shi, Bentao,et al."High expression of KPNA2 defines poor prognosis in patients with upper tract urothelial carcinoma treated with radical nephroureterectomy".BMC CANCER 15(2015).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Shi, Bentao]的文章
[Su, Boxing]的文章
[Fang, Dong]的文章
百度学术
百度学术中相似的文章
[Shi, Bentao]的文章
[Su, Boxing]的文章
[Fang, Dong]的文章
必应学术
必应学术中相似的文章
[Shi, Bentao]的文章
[Su, Boxing]的文章
[Fang, Dong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。