IR@PKUHSC  > 北京大学深圳医院  > 泌尿外科
学科主题临床医学
High expression of KPNA2 defines poor prognosis in patients with upper tract urothelial carcinoma treated with radical nephroureterectomy
Shi, Bentao1,2; Su, Boxing2,3; Fang, Dong2,3; Tang, Yuan2,3; Xiong, Gengyan2,3; Guo, Zhongqiang2,3; He, Qun2,4,5; Yang, Xinyu2,4,5; Zhao, Wei6; Guo, Yinglu2,3; Li, Xuesong2,3; Zhou, Liqun2,3
关键词Kpna2 Upper Tract Urothelial Carcinoma Prognosis Bladder Recurrence
刊名BMC CANCER
2015-05-09
DOI10.1186/s12885-015-1369-8
15
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者Beijing Natural Science Foundation ; Peking University Health Science Center ; National Taiwan University, College of Medicine ; National Natural Science Foundation ; Medical Scientific Research Foundation of Guangdong Province, China ; Science and Technology Project in Shenzhen ; Beijing Natural Science Foundation ; Peking University Health Science Center ; National Taiwan University, College of Medicine ; National Natural Science Foundation ; Medical Scientific Research Foundation of Guangdong Province, China ; Science and Technology Project in Shenzhen
研究领域[WOS]Oncology
关键词[WOS]UPPER URINARY-TRACT ; TRANSITIONAL-CELL CARCINOMA ; NUCLEAR-TRANSPORT RECEPTOR ; INTRAVESICAL RECURRENCE ; KARYOPHERIN ALPHA-2 ; PREDICTIVE FACTORS ; SUBUNIT ALPHA-2 ; PROTEIN IMPORT ; BREAST-CANCER ; LUNG-CANCER
英文摘要

Background: To analyze the expression of karyopherin alpha 2 (KPNA2) in upper tract urothelial carcinoma (UTUC) and to investigate whether the KPNA2 expression provides additional prognostic information following radical nephroureterectomy (RNU).

Methods: A tissue microarray (TMA) containing samples from 176 patients with UTUC who underwent RNU at our institute was analyzed for KPNA2 expression using immunohistochemistry. KPNA2 expression in normal urothelial cell line and urothelial carcinoma cell lines was evaluated by western blot analysis. Using RNA interference in vitro, the effects of KPNA2 inhibition on cellular viability, migration and apoptosis were determined.

Results: KPNA2 expression was significantly upregulated in the UTUC samples compared with the adjacent normal urothelial tissues. High KPNA2 immunoreactivity was identified as a predictor of bladder recurrence (hazard ratio [HR]: 2.017, 95% CI 1.13-3.61, p = 0.018), poor disease-free survival (DFS, HR: 2.754, 95% CI 1.68-4.51, p = 0.001) and poor overall survival (OS, HR: 4.480, 95% CI 1.84-10.89, p = 0.001) for patients with UTUC after RNU. Furthermore, high KPNA2 immunoreactivity was independent of the conventional predictive factors in a multivariate analysis. Additional in vitro experiments revealed that KPNA2 expression was higher in urothelial carcinoma cell lines than in normal urothelial cell line. KPNA2 inhibition with a specific siRNA decreased cell viability and migration and increased apoptosis in urothelial carcinoma cell lines.

Conclusions: KPNA2 is a novel independent prognostic marker for bladder recurrence, DFS and OS of UTUC patients who have undergone RNU. Moreover, these data suggest that KPNA2 may be a promising therapeutic target for UTUC.

语种英语
所属项目编号7122183 ; BMU20120318 ; BMU20120318 ; 81172419 ; 81372746 ; A2014653 ; 201302052
资助者Beijing Natural Science Foundation ; Peking University Health Science Center ; National Taiwan University, College of Medicine ; National Natural Science Foundation ; Medical Scientific Research Foundation of Guangdong Province, China ; Science and Technology Project in Shenzhen ; Beijing Natural Science Foundation ; Peking University Health Science Center ; National Taiwan University, College of Medicine ; National Natural Science Foundation ; Medical Scientific Research Foundation of Guangdong Province, China ; Science and Technology Project in Shenzhen
WOS记录号WOS:000354429700001
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56499
Collection北京大学深圳医院_泌尿外科
作者单位1.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
3.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen 518036, Guangdong, Peoples R China
4.Peking Univ, Beijing & Inst Urol, Natl Urol Canc Ctr, Beijing 100034, Peoples R China
5.Peking Univ, Hosp 1, Dept Urol Pathol, Beijing 100034, Peoples R China
6.Peking Univ, Dept Cell Biol, Sch Oncol, Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China
Recommended Citation
GB/T 7714
Shi, Bentao,Su, Boxing,Fang, Dong,et al. High expression of KPNA2 defines poor prognosis in patients with upper tract urothelial carcinoma treated with radical nephroureterectomy[J]. BMC CANCER,2015,15.
APA Shi, Bentao.,Su, Boxing.,Fang, Dong.,Tang, Yuan.,Xiong, Gengyan.,...&Zhou, Liqun.(2015).High expression of KPNA2 defines poor prognosis in patients with upper tract urothelial carcinoma treated with radical nephroureterectomy.BMC CANCER,15.
MLA Shi, Bentao,et al."High expression of KPNA2 defines poor prognosis in patients with upper tract urothelial carcinoma treated with radical nephroureterectomy".BMC CANCER 15(2015).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Shi, Bentao]'s Articles
[Su, Boxing]'s Articles
[Fang, Dong]'s Articles
百度学术
百度学术Similar articles in
[Shi, Bentao]'s Articles
[Su, Boxing]'s Articles
[Fang, Dong]'s Articles
必应学术
必应学术Similar articles in
[Shi, Bentao]'s Articles
[Su, Boxing]'s Articles
[Fang, Dong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.