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学科主题临床医学
Sulfur dioxide preconditioning increases antioxidative capacity in rat with myocardial ischemia reperfusion (I/R) injury
Jin, Hong Fang1; Wang, Yuan1; Wang, Xin Bao1; Sun, Yan1; Tang, Chao Shu2; Du, Jun Bao1,2
关键词Sulfur Dioxide Ischemia/reperfusion Preconditioning Oxidative Stress
刊名NITRIC OXIDE-BIOLOGY AND CHEMISTRY
2013-08-01
DOI10.1016/j.niox.2013.04.008
32页:56-61
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
资助者Major Basic Research Program of China ; National Natural Science Foundation of China ; Major Basic Research Program of China ; National Natural Science Foundation of China
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]NITRIC-OXIDE ; CONTAINING GASES ; LIPID PEROXIDES ; SERUM SULFITE ; GLUTATHIONE ; DERIVATIVES ; MECHANISMS ; PROTECTION ; STRESS ; METABOLISM
英文摘要

Background: The study was designed to explore if sulfur dioxide (SO2) preconditioning increased antioxidative capacity in rat with myocardial ischemia reperfusion (I/R) injury.

Methods: The myocardial I/R model was made by left coronary artery ligation for 30 min and reperfusion for 120 min in rats. Myocardial infarct size and plasma lactate dehydrogenase (LDH) and creatine kinase (CK) activities, plasma superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and glutathione (GSH) changes were detected for the rats. The contents of myocardial hydrogen sulfide (H2S) and nitric oxide (NO) were measured. Myocardial protein expressions of SOD1, SOD2, cystathionine gamma-lyase (CSE) and iNOS were tested using Western blot.

Results: Myocardial infarction developed and plasma CM and LDH activities were significantly increased in I/R group compared with those in control group, but SO2 preconditioning significantly reduced myocardial infarct size, and plasma CM and LDH activities. SO2 preconditioning successfully increased plasma SOD, GSH and GSH-Px levels and myocardial SOD1 protein expression, but decreased MDA level in rats of I/R group. Compared with controls, the myocardial H2S level and CSE expression were decreased after I/R, but myocardial NO level and iNOS expression were increased. With the treatment of SO2, myocardial H2S level and CSE expression were increased, but myocardial NO level and iNOS expression were decreased compared with those in I/R group.

Conclusions: SO2 preconditioning could significantly reduce I/R-induced myocardial injury in vivo in association with increased myocardial antioxidative capacity, upregulated myocardial H2S/CSE pathway but downregulated NO/iNOS pathway. (c) 2013 Elsevier Inc. All rights reserved.

语种英语
所属项目编号2012CB517806 ; 2011CB503904 ; 30821001 ; 81070111 ; 81070212
资助者Major Basic Research Program of China ; National Natural Science Foundation of China ; Major Basic Research Program of China ; National Natural Science Foundation of China
WOS记录号WOS:000320418800008
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56507
Collection北京大学第一临床医学院_儿科
作者单位1.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Jin, Hong Fang,Wang, Yuan,Wang, Xin Bao,et al. Sulfur dioxide preconditioning increases antioxidative capacity in rat with myocardial ischemia reperfusion (I/R) injury[J]. NITRIC OXIDE-BIOLOGY AND CHEMISTRY,2013,32:56-61.
APA Jin, Hong Fang,Wang, Yuan,Wang, Xin Bao,Sun, Yan,Tang, Chao Shu,&Du, Jun Bao.(2013).Sulfur dioxide preconditioning increases antioxidative capacity in rat with myocardial ischemia reperfusion (I/R) injury.NITRIC OXIDE-BIOLOGY AND CHEMISTRY,32,56-61.
MLA Jin, Hong Fang,et al."Sulfur dioxide preconditioning increases antioxidative capacity in rat with myocardial ischemia reperfusion (I/R) injury".NITRIC OXIDE-BIOLOGY AND CHEMISTRY 32(2013):56-61.
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