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学科主题基础医学
Characterization of multivalent lactose quantum dots and its application in carbohydrate-protein interactions study and cell imaging
Yang, Yang3; Yu, Min1,2; Yan, Ting-Ting4; Zhao, Zhi-Hui4; Sha, Yin-Lin1,2; Li, Zhong-Jun3
关键词Glycoquantum dots NMR SPR Multivalency 1-Thiol-lactose
刊名BIOORGANIC & MEDICINAL CHEMISTRY
2010-07-15
DOI10.1016/j.bmc.2010.05.046
18期:14页:5234-5240
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Chemistry, Organic
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Chemistry
关键词[WOS]ENCAPSULATED GOLD NANOPARTICLES ; BINDING ; GLYCONANOPARTICLES ; NANOCRYSTALS ; RECOGNITION ; MODEL ; OLIGOSACCHARIDES ; GLYCOSYLATION ; LIGANDS ; LECTINS
英文摘要

We have previously reported a facile and convenient method for the preparation of a new type of lactose-CdSeS/ZnS quantum dots conjugates (Lac-QDs) that exhibit biocompatibility, noncytotoxicity and specificity to leukocytes. In order to further study the carbohydrate-protein interactions, a series of Lac-QDs with different lactose densities and a PEGylated (n = 3) lactose-QDs conjugate (LacPEG-QDs) with more flexible sugar ligands were prepared. The amount of the sugar molecules on QDs can be determined by NMR, which was in agreement with the results from TGA determination. The formula of the conjugates was determined with ICP-OES. The interactions between the conjugated QDs and the PNA protein were measured using SPR, which revealed that higher lactose density favored binding affinity under the same concentration, and Lac-QDs exhibit higher affinity than LacPEG-QDs. We further used a solid phase assay to assess the anti-adhesion activity of Lac-QDs and LacPEG-QDs on the cell level. The results showed that Lac-QDs had stronger activity in preventing THP1 from adhering to HUVEC than LacPEGQDs, which was consistent with the SPR results. We reasoned that decrease in the conformational entropy induced by appropriate restriction of sugar flexibility could enhance the binding affinity of glyco-QDs, which implies that entropy change may be the main contributor to the interaction between high valent glyco-QDs and protein. The fabrication of lactose on QDs provides a fluorescent multivalent carbohydrate probe that can be used as mimics of glycoprotein for the study of carbohydrate-protein interactions and cell imaging. (C) 2010 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000279744700041
项目编号90713004 ; 2009ZX09103-044
资助机构Natural Science Foundation of China ; State New Drug Innovation ; State Key Laboratory of Natural and Biomimetic Drugs of Peking University
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56511
专题北京大学基础医学院_生物物理学系
北京大学基础医学院
北京大学药学院_化学生物学系
北京大学第一临床医学院_感染疾病科
作者单位1.Peking Univ, Biomed X Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Single Mol & Nanobiol Lab, Dept Biophys, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
4.Nanjing Normal Univ, Coll Life Sci, Nanjing 210046, Peoples R China
推荐引用方式
GB/T 7714
Yang, Yang,Yu, Min,Yan, Ting-Ting,et al. Characterization of multivalent lactose quantum dots and its application in carbohydrate-protein interactions study and cell imaging[J]. BIOORGANIC & MEDICINAL CHEMISTRY,2010,18(14):5234-5240.
APA Yang, Yang,Yu, Min,Yan, Ting-Ting,Zhao, Zhi-Hui,Sha, Yin-Lin,&Li, Zhong-Jun.(2010).Characterization of multivalent lactose quantum dots and its application in carbohydrate-protein interactions study and cell imaging.BIOORGANIC & MEDICINAL CHEMISTRY,18(14),5234-5240.
MLA Yang, Yang,et al."Characterization of multivalent lactose quantum dots and its application in carbohydrate-protein interactions study and cell imaging".BIOORGANIC & MEDICINAL CHEMISTRY 18.14(2010):5234-5240.
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