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学科主题: 基础医学
题名:
Characterization of multivalent lactose quantum dots and its application in carbohydrate-protein interactions study and cell imaging
作者: Yang, Yang3; Yu, Min1,2; Yan, Ting-Ting4; Zhao, Zhi-Hui4; Sha, Yin-Lin1,2; Li, Zhong-Jun3
关键词: Glycoquantum dots ; NMR ; SPR ; Multivalency ; 1-Thiol-lactose
刊名: BIOORGANIC & MEDICINAL CHEMISTRY
发表日期: 2010-07-15
DOI: 10.1016/j.bmc.2010.05.046
卷: 18, 期:14, 页:5234-5240
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Chemistry, Organic
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Chemistry
关键词[WOS]: ENCAPSULATED GOLD NANOPARTICLES ; BINDING ; GLYCONANOPARTICLES ; NANOCRYSTALS ; RECOGNITION ; MODEL ; OLIGOSACCHARIDES ; GLYCOSYLATION ; LIGANDS ; LECTINS
英文摘要:

We have previously reported a facile and convenient method for the preparation of a new type of lactose-CdSeS/ZnS quantum dots conjugates (Lac-QDs) that exhibit biocompatibility, noncytotoxicity and specificity to leukocytes. In order to further study the carbohydrate-protein interactions, a series of Lac-QDs with different lactose densities and a PEGylated (n = 3) lactose-QDs conjugate (LacPEG-QDs) with more flexible sugar ligands were prepared. The amount of the sugar molecules on QDs can be determined by NMR, which was in agreement with the results from TGA determination. The formula of the conjugates was determined with ICP-OES. The interactions between the conjugated QDs and the PNA protein were measured using SPR, which revealed that higher lactose density favored binding affinity under the same concentration, and Lac-QDs exhibit higher affinity than LacPEG-QDs. We further used a solid phase assay to assess the anti-adhesion activity of Lac-QDs and LacPEG-QDs on the cell level. The results showed that Lac-QDs had stronger activity in preventing THP1 from adhering to HUVEC than LacPEGQDs, which was consistent with the SPR results. We reasoned that decrease in the conformational entropy induced by appropriate restriction of sugar flexibility could enhance the binding affinity of glyco-QDs, which implies that entropy change may be the main contributor to the interaction between high valent glyco-QDs and protein. The fabrication of lactose on QDs provides a fluorescent multivalent carbohydrate probe that can be used as mimics of glycoprotein for the study of carbohydrate-protein interactions and cell imaging. (C) 2010 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 90713004 ; 2009ZX09103-044
项目资助者: Natural Science Foundation of China ; State New Drug Innovation ; State Key Laboratory of Natural and Biomimetic Drugs of Peking University
WOS记录号: WOS:000279744700041
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56511
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Biomed X Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Single Mol & Nanobiol Lab, Dept Biophys, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
4.Nanjing Normal Univ, Coll Life Sci, Nanjing 210046, Peoples R China

Recommended Citation:
Yang, Yang,Yu, Min,Yan, Ting-Ting,et al. Characterization of multivalent lactose quantum dots and its application in carbohydrate-protein interactions study and cell imaging[J]. BIOORGANIC & MEDICINAL CHEMISTRY,2010,18(14):5234-5240.
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