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学科主题: 公共卫生
题名:
Molecular cloning and expression analysis of srd5 alpha 2l2: a novel mouse member of steroid 5 alpha-reductase family
作者: Chen, XG; Li, Y; Zhang, DL; Cheng, J; Zhu, WL; Dao, JJ
关键词: Srd5 alpha 2l2 ; steroid 5 alpha-reductase ; novel gene ; heart specific
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2003-12-01
卷: 30, 期:6, 页:894-899
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: SEQUENCE TAGS ; REGION ; GENE
英文摘要:

A novel mouse heart-specific gene, Srd5alpha212, was cloned from mouse embryo heart by EST database searching and RT-PCR. The cDNA of Srd5alpha212 was 2 348 bp and composed of 12 extrons within over 77 kb in mouse genome. This gene was mapped to mouse chromosome 5 by BLAST search to mouse genome. Analysis of the 3′ untranslation region of the cDNA showed the presence of ATTTA sequence, which was believed to act as degradation signal of mRNA. The longest ORF spanned all of the 12 extrons and encoded a putative protein of 361 amino acids. A conserved steroid 5 alpha-reductase domain (3-oxo-5-alpha-steroid 4-dehydrogenase, STEROID- DH) was present at the C-terminus of the deduced protein. Homologous alignment found high identity of nucleotide sequence, deduced amino acid sequence and genomic structure between Srd5alpha212 and human cDNA of DKFZp313D0829 (AL833108). It may be concluded that AL833108 was the human orthologue of Srd5alpha212. BLAST search showed that cDNA of Srd5alpha212 was supported by 41 ESTs from EST database, 25 out of the 41 ESTs were from mouse heart. RT-PCR performed on a panel of different mouse tissues demonstrated that Srd5alpha212 strongly expressed in mouse heart but not or much more weakly expressed in other tissues. These results suggested that Srd5alpha212 is a novel heart-specific member of steroid 5 alpha-reductase family. As far as it is known that no heart-specific member of this family was reported before.

语种: 中文
WOS记录号: WOS:000224108900015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56538
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: 1.Peking Univ, Lab Mol Tox & Dev Mol Biol, Sch Publ Hlth, Dept Nutr & Food Hyg, Beijing 100083, Peoples R China
2.Sichuan Univ Sci & Technol, Dept Bioengn, Chengdu 610039, Peoples R China
3.Tsing Hua Univ, Sch Informat Sci & Technol, Dept Automat, State Key Lab Intelligent, Beijing 100084, Peoples R China

Recommended Citation:
Chen, XG,Li, Y,Zhang, DL,et al. Molecular cloning and expression analysis of srd5 alpha 2l2: a novel mouse member of steroid 5 alpha-reductase family[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2003,30(6):894-899.
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