|Characterization of Streptococcus oligofermentans sucrose metabolism demonstrates reduced pyruvic and lactic acid production|
|Bao Xu-dong1,2; Yue Lin1,2; Gao Xue-jun1,2|
|关键词||Streptococcus Oligofermentans Lactate Dehydrogenase Pyruvic Acid Lactic Acid|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||LACTATE-DEHYDROGENASE ; FERMENTATION PRODUCTS ; MUTANS ; PLAQUE ; H2O2|
Background Streptococcus (S.) oligofermentans is a newly identified bacteria with a yet to be defined mechanism of sucrose metabolism that results in acid production. This study aimed to investigate the biochemical mechanisms of S. oligoferm-entans glucose metaolism.
Methods The S. oligofermentans LMG21532, Lactobacillus (L.) fermentum 38 and the S. mutans UA140 were used to characterize sucrose metabolism by measuring lactate dehydrogenase (LDH) activity and lactic acid production. Continuous dynamics and high performance capillary electrophoresis were used to determine LDH activity and lactic acid production, respectively, from bacteria collected at 0, 10 and 30 minutes after cultured in 10% sucrose.
Results These analyses demonstrated that LDH activity of the three bacterial strains examined remained stable but significantly different throughout the sucrose fermentation process. The S. oligofermentans LDH activity ((0.61 +/- 0.05) U/mg) was significantly lower than that of L. ferment urn ((52.91 +/- 8.97) U/mg). In addition, the S. oligofermentans total lactate production ((0.048 +/- 0.021) mmol/L) was also significantly lower than that of L. fermentum ((0.958 +/- 0.201) mmol/L). Although the S. oligofermentans LDH production was almost double of that produced by S. mutans ((0.32 +/- 0.07) U/mg), lactic acid production was approximately one sixth that of S. mutans ((0.296 +/- 0.058) mmol/L). Additional tests examining pyruvic acid production (the LDH substrate) demonstrated that lactic acid concentrations correlated with pyruvic acid production. That is, pyruvic acid production by S. oligofermentans was undetectable following sucrose incubation, however, (0.074 +/- 0.024) and (0.175 +/- 0.098) mmol/L pyruvic acid were produced by S. mutans and L. fermentum, respectively.
Conclusion S. oligofermentans is incapable of fermenting carbohydrates to produce enough pyruvic acid, which results in reduced lactic acid production. Chin Med J 2011;124(21):3499-3503
|项目编号||2007BAI18B01 ; 8107088 ; 7102163|
|资助机构||Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation|
|作者单位||1.Peking Univ, Sch Stomatol, Dept Cariol & Endodontol, Beijing 100081, Peoples R China|
2.Peking Univ, Hosp Stomatol, Dept Cariol & Endodontol, Beijing 100081, Peoples R China
|Bao Xu-dong,Yue Lin,Gao Xue-jun. Characterization of Streptococcus oligofermentans sucrose metabolism demonstrates reduced pyruvic and lactic acid production[J]. CHINESE MEDICAL JOURNAL,2011,124(21):3499-3503.|
|APA||Bao Xu-dong,Yue Lin,&Gao Xue-jun.(2011).Characterization of Streptococcus oligofermentans sucrose metabolism demonstrates reduced pyruvic and lactic acid production.CHINESE MEDICAL JOURNAL,124(21),3499-3503.|
|MLA||Bao Xu-dong,et al."Characterization of Streptococcus oligofermentans sucrose metabolism demonstrates reduced pyruvic and lactic acid production".CHINESE MEDICAL JOURNAL 124.21(2011):3499-3503.|