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Characterization of Streptococcus oligofermentans sucrose metabolism demonstrates reduced pyruvic and lactic acid production
Bao Xu-dong1,2; Yue Lin1,2; Gao Xue-jun1,2
关键词Streptococcus Oligofermentans Lactate Dehydrogenase Pyruvic Acid Lactic Acid
刊名CHINESE MEDICAL JOURNAL
2011-11-05
DOI10.3760/cma.j.issn.0366-6999.2011.21.012
124期:21页:3499-3503
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]LACTATE-DEHYDROGENASE ; FERMENTATION PRODUCTS ; MUTANS ; PLAQUE ; H2O2
英文摘要

Background Streptococcus (S.) oligofermentans is a newly identified bacteria with a yet to be defined mechanism of sucrose metabolism that results in acid production. This study aimed to investigate the biochemical mechanisms of S. oligoferm-entans glucose metaolism.

Methods The S. oligofermentans LMG21532, Lactobacillus (L.) fermentum 38 and the S. mutans UA140 were used to characterize sucrose metabolism by measuring lactate dehydrogenase (LDH) activity and lactic acid production. Continuous dynamics and high performance capillary electrophoresis were used to determine LDH activity and lactic acid production, respectively, from bacteria collected at 0, 10 and 30 minutes after cultured in 10% sucrose.

Results These analyses demonstrated that LDH activity of the three bacterial strains examined remained stable but significantly different throughout the sucrose fermentation process. The S. oligofermentans LDH activity ((0.61 +/- 0.05) U/mg) was significantly lower than that of L. ferment urn ((52.91 +/- 8.97) U/mg). In addition, the S. oligofermentans total lactate production ((0.048 +/- 0.021) mmol/L) was also significantly lower than that of L. fermentum ((0.958 +/- 0.201) mmol/L). Although the S. oligofermentans LDH production was almost double of that produced by S. mutans ((0.32 +/- 0.07) U/mg), lactic acid production was approximately one sixth that of S. mutans ((0.296 +/- 0.058) mmol/L). Additional tests examining pyruvic acid production (the LDH substrate) demonstrated that lactic acid concentrations correlated with pyruvic acid production. That is, pyruvic acid production by S. oligofermentans was undetectable following sucrose incubation, however, (0.074 +/- 0.024) and (0.175 +/- 0.098) mmol/L pyruvic acid were produced by S. mutans and L. fermentum, respectively.

Conclusion S. oligofermentans is incapable of fermenting carbohydrates to produce enough pyruvic acid, which results in reduced lactic acid production. Chin Med J 2011;124(21):3499-3503

语种英语
WOS记录号WOS:000297658200013
项目编号2007BAI18B01 ; 8107088 ; 7102163
资助机构Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56541
专题北京大学口腔医学院
北京大学口腔医学院_牙体牙髓科
作者单位1.Peking Univ, Sch Stomatol, Dept Cariol & Endodontol, Beijing 100081, Peoples R China
2.Peking Univ, Hosp Stomatol, Dept Cariol & Endodontol, Beijing 100081, Peoples R China
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Bao Xu-dong,Yue Lin,Gao Xue-jun. Characterization of Streptococcus oligofermentans sucrose metabolism demonstrates reduced pyruvic and lactic acid production[J]. CHINESE MEDICAL JOURNAL,2011,124(21):3499-3503.
APA Bao Xu-dong,Yue Lin,&Gao Xue-jun.(2011).Characterization of Streptococcus oligofermentans sucrose metabolism demonstrates reduced pyruvic and lactic acid production.CHINESE MEDICAL JOURNAL,124(21),3499-3503.
MLA Bao Xu-dong,et al."Characterization of Streptococcus oligofermentans sucrose metabolism demonstrates reduced pyruvic and lactic acid production".CHINESE MEDICAL JOURNAL 124.21(2011):3499-3503.
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