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学科主题临床医学
Efficient episomal reprogramming of blood mononuclear cells and differentiation to hepatocytes with functional drug metabolism
Liu, Jing1,2,3; Brzeszczynska, Joanna2; Samuel, Kay1; Black, Jim2; Palakkan, Anwar2; Anderson, Richard A.4; Gallagher, Ronald1; Ross, James A.2
关键词Stem Cell Reprogramming Episomal Pluripotent Hepatocyte
刊名EXPERIMENTAL CELL RESEARCH
2015-11-01
DOI10.1016/j.yexcr.2015.08.004
338期:2页:203-213
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Cell Biology
研究领域[WOS]Oncology ; Cell Biology
关键词[WOS]PLURIPOTENT STEM-CELLS ; HUMAN FIBROBLASTS ; HEPATIC ENDODERM ; GENE-EXPRESSION ; CORD BLOOD ; GENERATION ; MOUSE ; P53
英文摘要

The possibility of converting cells from blood mononuclear cells (MNC) to liver cells provides promising opportunities for the study of diseases and the assessment of new drugs. However, clinical applications have to meet GMP requirements and the methods for generating induced pluripotent cells (iPCs) have to avoid insertional mutagenesis, a possibility when using viral vehicles for the delivery of reprogramming factors. We have developed an efficient non-integration method for reprogramming fresh or frozen blood MNC, maintained in an optimised cytokine cocktail, to generate induced pluripotent cells. Using electroporation for the effective delivery of episomal transcription factors (Oct4, Sox2, K1f4, L-Myc, and Lin28) in a feeder-free system, without any requirement for small molecules, we achieved a reprogramming efficiency of up to 0.033% (65 colonies from 2 x 10(5) seeded MNC). Applying the same cytokine cocktail and reprogramming methods to cord blood or fetal liver-derived CD34(+) cells, we obtained 148 iPS colonies from 10(5) seeding cells (0.148%). The iPS cell lines we generated maintained typical characteristics of pluripotent cells and could be successfully differentiated into hepatocytes with drug metabolic function. (C) 2015 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000364354500008
项目编号2009601103
资助机构China Scholarship Council
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56544
专题北京大学第二临床医学院_血液科
作者单位1.Ctr Regenerat Med, SNBTS, Cell Therapy Grp, Edinburgh EH16 4SB, Midlothian, Scotland
2.Univ Edinburgh, MRC Ctr Regenerat Med, Tissue Injury & Repair Grp, Edinburgh EH16 4SB, Midlothian, Scotland
3.Peking Univ, Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China
4.Univ Edinburgh, MRC Ctr Reprod Hlth, Edinburgh EH16 4TJ, Midlothian, Scotland
推荐引用方式
GB/T 7714
Liu, Jing,Brzeszczynska, Joanna,Samuel, Kay,et al. Efficient episomal reprogramming of blood mononuclear cells and differentiation to hepatocytes with functional drug metabolism[J]. EXPERIMENTAL CELL RESEARCH,2015,338(2):203-213.
APA Liu, Jing.,Brzeszczynska, Joanna.,Samuel, Kay.,Black, Jim.,Palakkan, Anwar.,...&Ross, James A..(2015).Efficient episomal reprogramming of blood mononuclear cells and differentiation to hepatocytes with functional drug metabolism.EXPERIMENTAL CELL RESEARCH,338(2),203-213.
MLA Liu, Jing,et al."Efficient episomal reprogramming of blood mononuclear cells and differentiation to hepatocytes with functional drug metabolism".EXPERIMENTAL CELL RESEARCH 338.2(2015):203-213.
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