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IR@PKUHSC  > 北京大学第二临床医学院  > 血液科  > 期刊论文
学科主题: 临床医学
题名:
Efficient episomal reprogramming of blood mononuclear cells and differentiation to hepatocytes with functional drug metabolism
作者: Liu, Jing1,2,3; Brzeszczynska, Joanna2; Samuel, Kay1; Black, Jim2; Palakkan, Anwar2; Anderson, Richard A.4; Gallagher, Ronald1; Ross, James A.2
关键词: Stem cell ; Reprogramming ; Episomal ; Pluripotent ; Hepatocyte
刊名: EXPERIMENTAL CELL RESEARCH
发表日期: 2015-11-01
DOI: 10.1016/j.yexcr.2015.08.004
卷: 338, 期:2, 页:203-213
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Cell Biology
研究领域[WOS]: Oncology ; Cell Biology
关键词[WOS]: PLURIPOTENT STEM-CELLS ; HUMAN FIBROBLASTS ; HEPATIC ENDODERM ; GENE-EXPRESSION ; CORD BLOOD ; GENERATION ; MOUSE ; P53
英文摘要:

The possibility of converting cells from blood mononuclear cells (MNC) to liver cells provides promising opportunities for the study of diseases and the assessment of new drugs. However, clinical applications have to meet GMP requirements and the methods for generating induced pluripotent cells (iPCs) have to avoid insertional mutagenesis, a possibility when using viral vehicles for the delivery of reprogramming factors. We have developed an efficient non-integration method for reprogramming fresh or frozen blood MNC, maintained in an optimised cytokine cocktail, to generate induced pluripotent cells. Using electroporation for the effective delivery of episomal transcription factors (Oct4, Sox2, K1f4, L-Myc, and Lin28) in a feeder-free system, without any requirement for small molecules, we achieved a reprogramming efficiency of up to 0.033% (65 colonies from 2 x 10(5) seeded MNC). Applying the same cytokine cocktail and reprogramming methods to cord blood or fetal liver-derived CD34(+) cells, we obtained 148 iPS colonies from 10(5) seeding cells (0.148%). The iPS cell lines we generated maintained typical characteristics of pluripotent cells and could be successfully differentiated into hepatocytes with drug metabolic function. (C) 2015 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 2009601103
项目资助者: China Scholarship Council
WOS记录号: WOS:000364354500008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56544
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

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作者单位: 1.Ctr Regenerat Med, SNBTS, Cell Therapy Grp, Edinburgh EH16 4SB, Midlothian, Scotland
2.Univ Edinburgh, MRC Ctr Regenerat Med, Tissue Injury & Repair Grp, Edinburgh EH16 4SB, Midlothian, Scotland
3.Peking Univ, Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China
4.Univ Edinburgh, MRC Ctr Reprod Hlth, Edinburgh EH16 4TJ, Midlothian, Scotland

Recommended Citation:
Liu, Jing,Brzeszczynska, Joanna,Samuel, Kay,et al. Efficient episomal reprogramming of blood mononuclear cells and differentiation to hepatocytes with functional drug metabolism[J]. EXPERIMENTAL CELL RESEARCH,2015,338(2):203-213.
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