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学科主题: 临床医学
题名:
Antiproliferative effects of anastrozole on MCF-7 human breast cancer cells in vitro are significantly enhanced by combined treatment with testosterone undecanoate
作者: Chen, Rong; Cui, Junwei; Wang, Qinqin; Li, Peng; Liu, Xiaoling; Hu, Hui; Wei, Wei
关键词: breast cancer ; anastrozole ; testosterone undecanoate ; androgen receptor
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2015-07-01
DOI: 10.3892/mmr.2015.3427
卷: 12, 期:1, 页:769-775
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: ANDROGEN RECEPTOR ; THERAPEUTIC STRATEGIES ; AROMATASE INHIBITOR ; ESTROGEN ; EXPRESSION ; WOMEN ; TRANSCRIPTION ; RESISTANCE ; PROGRAM ; GROWTH
英文摘要:

The present study aimed to assess the effects of aromatase inhibitor anastrozole and testosterone undecanoate, separately and in combination, on proliferation and apoptosis in MCF-7 human breast cancer cells cultured in vitro. The effects of various concentrations of these drugs on the proliferation of MCF-7 cells were evaluated by CCK8 assay, the levels of cell apoptosis were evaluated by flow cytometry with Annexin-V/propidium iodide staining and androgen receptor (AR) protein expression was determined by western blot analysis. The results of the CCK8 assay indicated that greater antiproliferative activity was detected in the MCF-7 cells in the combined treatment groups, compared with those treated with anastrozole or testosterone undecanoate alone. Flow cytometric analysis of apoptosis revealed that treatment with a combination of the two drugs generated a higher percentage of apoptotic cells, particularly when the two drugs were applied for 48 h, compared with single drug treatment. Western blot analysis revealed a significant decrease in AR protein expression in the combined treatment groups compared with MCF7 cells treated with single drugs. The results of the present study provided evidence supporting the potential of a combination of anastrozole and testosterone undecanoate as a novel therapeutic strategy for the treatment of breast cancer. Furthermore, it was demonstrated that the antiproliferative effects of anastrozole were significantly enhanced by combined treatment with testosterone undecanoate via the AR signaling pathway.

语种: 英语
所属项目编号: JCYJ20130402114702122 ; 201103010
项目资助者: Shenzhen Science and Research Innovation Foundation ; Shenzhen Science and Technology Plan Projects
WOS记录号: WOS:000358134400110
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56564
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: Peking Univ, Dept Breast Surg, Shenzhen Hosp, Shenzhen 518036, Guangdong, Peoples R China

Recommended Citation:
Chen, Rong,Cui, Junwei,Wang, Qinqin,et al. Antiproliferative effects of anastrozole on MCF-7 human breast cancer cells in vitro are significantly enhanced by combined treatment with testosterone undecanoate[J]. MOLECULAR MEDICINE REPORTS,2015,12(1):769-775.
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