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Antiproliferative effects of anastrozole on MCF-7 human breast cancer cells in vitro are significantly enhanced by combined treatment with testosterone undecanoate
Chen, Rong; Cui, Junwei; Wang, Qinqin; Li, Peng; Liu, Xiaoling; Hu, Hui; Wei, Wei
关键词Breast Cancer Anastrozole Testosterone Undecanoate Androgen Receptor
刊名MOLECULAR MEDICINE REPORTS
2015-07-01
DOI10.3892/mmr.2015.3427
12期:1页:769-775
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]ANDROGEN RECEPTOR ; THERAPEUTIC STRATEGIES ; AROMATASE INHIBITOR ; ESTROGEN ; EXPRESSION ; WOMEN ; TRANSCRIPTION ; RESISTANCE ; PROGRAM ; GROWTH
英文摘要

The present study aimed to assess the effects of aromatase inhibitor anastrozole and testosterone undecanoate, separately and in combination, on proliferation and apoptosis in MCF-7 human breast cancer cells cultured in vitro. The effects of various concentrations of these drugs on the proliferation of MCF-7 cells were evaluated by CCK8 assay, the levels of cell apoptosis were evaluated by flow cytometry with Annexin-V/propidium iodide staining and androgen receptor (AR) protein expression was determined by western blot analysis. The results of the CCK8 assay indicated that greater antiproliferative activity was detected in the MCF-7 cells in the combined treatment groups, compared with those treated with anastrozole or testosterone undecanoate alone. Flow cytometric analysis of apoptosis revealed that treatment with a combination of the two drugs generated a higher percentage of apoptotic cells, particularly when the two drugs were applied for 48 h, compared with single drug treatment. Western blot analysis revealed a significant decrease in AR protein expression in the combined treatment groups compared with MCF7 cells treated with single drugs. The results of the present study provided evidence supporting the potential of a combination of anastrozole and testosterone undecanoate as a novel therapeutic strategy for the treatment of breast cancer. Furthermore, it was demonstrated that the antiproliferative effects of anastrozole were significantly enhanced by combined treatment with testosterone undecanoate via the AR signaling pathway.

语种英语
WOS记录号WOS:000358134400110
项目编号JCYJ20130402114702122 ; 201103010
资助机构Shenzhen Science and Research Innovation Foundation ; Shenzhen Science and Technology Plan Projects
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56564
专题北京大学深圳医院
北京大学精神卫生研究所_精神科
作者单位Peking Univ, Dept Breast Surg, Shenzhen Hosp, Shenzhen 518036, Guangdong, Peoples R China
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GB/T 7714
Chen, Rong,Cui, Junwei,Wang, Qinqin,et al. Antiproliferative effects of anastrozole on MCF-7 human breast cancer cells in vitro are significantly enhanced by combined treatment with testosterone undecanoate[J]. MOLECULAR MEDICINE REPORTS,2015,12(1):769-775.
APA Chen, Rong.,Cui, Junwei.,Wang, Qinqin.,Li, Peng.,Liu, Xiaoling.,...&Wei, Wei.(2015).Antiproliferative effects of anastrozole on MCF-7 human breast cancer cells in vitro are significantly enhanced by combined treatment with testosterone undecanoate.MOLECULAR MEDICINE REPORTS,12(1),769-775.
MLA Chen, Rong,et al."Antiproliferative effects of anastrozole on MCF-7 human breast cancer cells in vitro are significantly enhanced by combined treatment with testosterone undecanoate".MOLECULAR MEDICINE REPORTS 12.1(2015):769-775.
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