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学科主题: 药物依赖
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Molecular chaperone heat shock protein 70 participates in the labile phase of the development of behavioural sensitization induced by a single morphine exposure in mice
作者: Qin, Wang-Jun1; Wang, Yan-Ting1; Zhang, Min1; Wen, Rui-Ting1; Liu, Qing1; Li, Yu-Ling1; Chen, Feng2,3; Lawrence, Andrew J.2,3; Liang, Jian-Hui1
关键词: Behavioural sensitization ; heat shock protein 70 ; labile phase ; morphine
刊名: INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY
发表日期: 2013-04-01
DOI: 10.1017/S1461145712000557
卷: 16, 期:3, 页:647-659
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Clinical Neurology ; Neurosciences ; Pharmacology & Pharmacy ; Psychiatry
研究领域[WOS]: Neurosciences & Neurology ; Pharmacology & Pharmacy ; Psychiatry
关键词[WOS]: NOVO PROTEIN-SYNTHESIS ; HEAT-SHOCK ; SYNAPTIC PLASTICITY ; NEURAL BASIS ; EXPRESSION ; ADDICTION ; HSP70 ; RAT ; BRAIN ; AMPHETAMINE
英文摘要:

De-novo protein synthesis is required in the development of behavioural sensitization. A prior screening test from our laboratory has implicated heat shock protein 70 (Hsp70) as one of the proteins required in this behavioural plasticity. Thus, this study was designed to extend our understanding of the role of Hsp70 in the development of behavioural sensitization induced by a single morphine exposure in mice. First, by employing transcription inhibitor actinomycin D (AD) and protein synthesis inhibitor cycloheximide (CHX), we identified a protein synthesis-dependent labile phase (within 4 h after the first morphine injection) in the development of behavioural sensitization to a single morphine exposure. Second, Hsp70 protein expression in the nucleus accumbens correlated positively with locomotor responses of sensitized mice and, more importantly, the expression of Hsp70 increased within 1 h after the first morphine injection. Third, AD and CHX both prevented expression of Hsp70 and disrupted the development of the single morphine induced behavioural sensitization, which further implied Hsp70 was highly associated with behavioural sensitization. Finally, the selective Hsp70 inhibitor pifithrin-m (PES) i.c.v. injected in mice prevented the development of behavioural sensitization and, critically, this inhibitory effect occurred only when PES was given within 1 h after the first morphine injection, which was within the labile phase of the development period. Taken together, we draw the conclusion that Hsp70 is crucially involved in the labile phase of the development of behavioural sensitization induced by a single morphine exposure, probably functioning as a molecular chaperone.

语种: 英语
所属项目编号: 30870894 ; 2009CB522000 ; 2011BAK04B08
项目资助者: National Nature Science Foundation of China ; National Basic Research Program of China ; National Key Technology R&amp ; D Program of China ; National Health &amp ; Medical Research Council (Australia)
WOS记录号: WOS:000315527600014
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56579
Appears in Collections:中国药物依赖性研究所_期刊论文

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作者单位: 1.Univ Melbourne, Ctr Neurosci, Parkville, Vic 3052, Australia
2.Peking Univ, Natl Inst Drug Dependence, Beijing 100191, Peoples R China
3.Univ Melbourne, Florey Neurosci Inst, Parkville, Vic 3052, Australia

Recommended Citation:
Qin, Wang-Jun,Wang, Yan-Ting,Zhang, Min,et al. Molecular chaperone heat shock protein 70 participates in the labile phase of the development of behavioural sensitization induced by a single morphine exposure in mice[J]. INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY,2013,16(3):647-659.
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