IR@PKUHSC  > 北京大学第三临床医学院  > 普通外科
学科主题临床医学
Metabonomic Analysis of Rats With Acute Heart Rejection
Tao, M.; Xiu, D. R.
刊名TRANSPLANTATION PROCEEDINGS
2013-03-01
DOI10.1016/j.transproceed.2012.06.085
45期:2页:618-624
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Surgery ; Transplantation
研究领域[WOS]Immunology ; Surgery ; Transplantation
关键词[WOS]FULMINANT HEPATIC-FAILURE ; BALB/C MICE ; TRANSPLANTATION ; RESPONSES
英文摘要

Background. Organs transplantation is an effective treatment for end-stage organ failure. Despite the use of modem immunosuppressants to decrease its incidence, acute rejection episodes (ARE), still present a problem for diagnosis, resolution, and prediction of long-term outcomes due to the absence of sufficiently robust biomarkers.

Methods. Using an heterotopic heart transplantation model using Dark Agouti to Lewis rats, and sirolimus (rapamycin, Rapa) treatment by gavage, we divided recipients into four groups: controls, ARE, Rapa-14, and Rapa-7. We evaluated recipients by hematoxylin and eosin staining of grafts and reverse transcription polymerase chain reactions. Levels of plasma metabolites were quantified using gas chromatography/time-of-flight mass spectrometry. Data were evaluated employing partial least-squares discriminant analysis (PLS-DA), the area under the receiver operating characteristic curves with negative predictive values (NPV) and positive predictive values (PPV).

Results. The graft survival was prolonged by Rapa. Plasma levels of 10 metabolites differed significantly between the ARE and the Rapa-14 groups as illustrated by the total ion current. According to PLS-DA, proline, glycine, serine, phenylalanine, and isocitrate showed the greatest effects with areas under the curve of 0.944, 0.917, 1.0, 0.861, and 0.944 respectively. The NPV values were 85.7%, 85.7%, 100%, 83.3%, and 85.7% and PPV values, 100%, 100%, 100%, 83.3%, and 100% respectively. Therefore, these metabolites may be used to predict the occurrence and progression of ARE.

Conclusion. The trend of changes suggested that plasma metabolites correlated with the immune state of recipients. Therefore, metabonomics may provide new biomarkers for graft injury in the early phases of ARE.

语种英语
WOS记录号WOS:000316772500026
项目编号30872556
资助机构National Natural Sciences Foundation of China
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56590
专题北京大学第三临床医学院_普通外科
作者单位Peking Univ, Hosp 3, Dept Gen Surg, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Tao, M.,Xiu, D. R.. Metabonomic Analysis of Rats With Acute Heart Rejection[J]. TRANSPLANTATION PROCEEDINGS,2013,45(2):618-624.
APA Tao, M.,&Xiu, D. R..(2013).Metabonomic Analysis of Rats With Acute Heart Rejection.TRANSPLANTATION PROCEEDINGS,45(2),618-624.
MLA Tao, M.,et al."Metabonomic Analysis of Rats With Acute Heart Rejection".TRANSPLANTATION PROCEEDINGS 45.2(2013):618-624.
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