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学科主题: 临床医学
题名:
Metabonomic Analysis of Rats With Acute Heart Rejection
作者: Tao, M.; Xiu, D. R.
刊名: TRANSPLANTATION PROCEEDINGS
发表日期: 2013-03-01
DOI: 10.1016/j.transproceed.2012.06.085
卷: 45, 期:2, 页:618-624
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Surgery ; Transplantation
研究领域[WOS]: Immunology ; Surgery ; Transplantation
关键词[WOS]: FULMINANT HEPATIC-FAILURE ; BALB/C MICE ; TRANSPLANTATION ; RESPONSES
英文摘要:

Background. Organs transplantation is an effective treatment for end-stage organ failure. Despite the use of modem immunosuppressants to decrease its incidence, acute rejection episodes (ARE), still present a problem for diagnosis, resolution, and prediction of long-term outcomes due to the absence of sufficiently robust biomarkers.

Methods. Using an heterotopic heart transplantation model using Dark Agouti to Lewis rats, and sirolimus (rapamycin, Rapa) treatment by gavage, we divided recipients into four groups: controls, ARE, Rapa-14, and Rapa-7. We evaluated recipients by hematoxylin and eosin staining of grafts and reverse transcription polymerase chain reactions. Levels of plasma metabolites were quantified using gas chromatography/time-of-flight mass spectrometry. Data were evaluated employing partial least-squares discriminant analysis (PLS-DA), the area under the receiver operating characteristic curves with negative predictive values (NPV) and positive predictive values (PPV).

Results. The graft survival was prolonged by Rapa. Plasma levels of 10 metabolites differed significantly between the ARE and the Rapa-14 groups as illustrated by the total ion current. According to PLS-DA, proline, glycine, serine, phenylalanine, and isocitrate showed the greatest effects with areas under the curve of 0.944, 0.917, 1.0, 0.861, and 0.944 respectively. The NPV values were 85.7%, 85.7%, 100%, 83.3%, and 85.7% and PPV values, 100%, 100%, 100%, 83.3%, and 100% respectively. Therefore, these metabolites may be used to predict the occurrence and progression of ARE.

Conclusion. The trend of changes suggested that plasma metabolites correlated with the immune state of recipients. Therefore, metabonomics may provide new biomarkers for graft injury in the early phases of ARE.

语种: 英语
所属项目编号: 30872556
项目资助者: National Natural Sciences Foundation of China
WOS记录号: WOS:000316772500026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56590
Appears in Collections:北京大学第三临床医学院_普通外科_期刊论文

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作者单位: Peking Univ, Hosp 3, Dept Gen Surg, Beijing 100871, Peoples R China

Recommended Citation:
Tao, M.,Xiu, D. R.. Metabonomic Analysis of Rats With Acute Heart Rejection[J]. TRANSPLANTATION PROCEEDINGS,2013,45(2):618-624.
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