IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
Silencing of miR-101 Prevents Cartilage Degradation by Regulating Extracellular Matrix-related Genes in a Rat Model of Osteoarthritis
Dai, Linghui1,2; Zhang, Xin1; Hu, Xiaoqing1; Liu, Qiang1; Man, Zhentao1; Huang, Hongjie1; Meng, Qingyang1; Zhou, Chunyan2; Ao, Yingfang1
刊名MOLECULAR THERAPY
2015-08-01
DOI10.1038/mt.2015.61
23期:8页:1331-1340
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
资助者China Postdoctoral Science Foundation ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; National Natural Science Foundation of China
研究领域[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine
关键词[WOS]HUMAN ARTICULAR CHONDROCYTES ; MOLECULAR-MECHANISMS ; EXPRESSION ; MICRORNAS ; DISEASE ; MANAGEMENT ; MORPHOLOGY ; ARTHRITIS ; SYSTEM
英文摘要

Osteoarthritis (OA) is a common, degenerative joint disease characterized by articular cartilage degradation. Currently, clinical trials based on microRNA therapy have been performed to treat various diseases. However, no treatment has been found for arthritis. This study investigated the functions of miR-101 in cartilage degradation in vivo and evaluated the feasibility of using miR-101 as a therapeutic agent for OA. Mono-iodoacetate-induced arthritis (MIA) rats were used as an animal model of OA. miR-101 mimic or miR-101 inhibitor was injected into the rats′ knees to evaluate its effects on cartilage degradation. Cartilage degradation aggravated at 14 days after the injection of miR-101 mimic. By contrast, miR-101 silencing reduced cartilage degradation. Moreover, the administration of miR-101 mimic is sufficient to cause cartilage degradation in the normal cartilage of rats. By contrast, miR-101 inhibitor could prevent this change. Microarray and qPCR were used to investigate the different expressed genes after injecting miR-101 mimic and miR-101 inhibitor in the rats′ articular cartilage. Several cartilage degradation-related genes were selected and validated to function in cartilage degradation with miR-101. Our results demonstrated the therapeutic effect of miR-101 inhibition on cartilage degradation in MIA rats by regulating several cartilage degradation-related genes.

语种英语
所属项目编号2014M550569 ; 81330040 ; 81370236
资助者China Postdoctoral Science Foundation ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; National Natural Science Foundation of China
WOS记录号WOS:000358843900007
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56626
专题北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Hosp 3, Inst Sports Med, Beijing Key Lab Sports Injuries, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Dai, Linghui,Zhang, Xin,Hu, Xiaoqing,et al. Silencing of miR-101 Prevents Cartilage Degradation by Regulating Extracellular Matrix-related Genes in a Rat Model of Osteoarthritis[J]. MOLECULAR THERAPY,2015,23(8):1331-1340.
APA Dai, Linghui.,Zhang, Xin.,Hu, Xiaoqing.,Liu, Qiang.,Man, Zhentao.,...&Ao, Yingfang.(2015).Silencing of miR-101 Prevents Cartilage Degradation by Regulating Extracellular Matrix-related Genes in a Rat Model of Osteoarthritis.MOLECULAR THERAPY,23(8),1331-1340.
MLA Dai, Linghui,et al."Silencing of miR-101 Prevents Cartilage Degradation by Regulating Extracellular Matrix-related Genes in a Rat Model of Osteoarthritis".MOLECULAR THERAPY 23.8(2015):1331-1340.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Dai, Linghui]的文章
[Zhang, Xin]的文章
[Hu, Xiaoqing]的文章
百度学术
百度学术中相似的文章
[Dai, Linghui]的文章
[Zhang, Xin]的文章
[Hu, Xiaoqing]的文章
必应学术
必应学术中相似的文章
[Dai, Linghui]的文章
[Zhang, Xin]的文章
[Hu, Xiaoqing]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。