IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Effects of serum from patients with early-onset pre-eclampsia, HELLP syndrome, and antiphospholipid syndrome on fatty acid oxidation in trophoblast cells
Yu, Huan1; Yang, Zi1; Ding, Xiaoyan1; Wang, Yanling2; Han, Yiwei1
关键词Fatty Acid Oxidation Trophoblast Cells Pre-eclampsia Hellp Syndrome
刊名ARCHIVES OF GYNECOLOGY AND OBSTETRICS
2015-09-01
DOI10.1007/s00404-015-3669-2
292期:3页:559-567
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology
资助者National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
研究领域[WOS]Obstetrics & Gynecology
关键词[WOS]LIPOPROTEIN
英文摘要

The role of metabolic disorders of long-chain fatty acid oxidation in the development of pre-eclampsia (PE), hemolysis, elevated liver enzymes, and low platelets (HELLP) syndrome and antiphospholipid syndrome (APS) is unclear. The aim of this study was to research the effects of three serum on fatty acid oxidation in trophoblast cells.

Primary human trophoblast cells and HTR8/SVneo cells were treated with serum from patients with early-onset severe PE (E-PE), E-PE with HELLP (PE-HELLP), APS, and normal pregnant women as controls (NC). Cells treated with free fatty acids (FFAs) of various lengths were used as controls.

Triglyceride (TG) and FFA levels of the E-PE and APS groups were significantly higher than the PE-HELLP and NC groups (P < 0.05). Trophoblast cells treated with serum from the E-PE and APS groups showed obvious morphological changes and a large amount of lipid droplet deposition. Cells in the E-PE group had more severe damage of mitochondria ultrastructure, presenting with cell morphological changes, lipid droplet deposition, and mitochondria damage similar to the long-chain FFA group. FFA levels in the PE-HELLP group increased significantly compared with the NC group (P < 0.05), while TG levels did not change significantly (P > 0.05). Trophoblast cells treated with serum from the PE-HELLP group showed cellular morphology and mitochondria changes similar to the E-PE group, but had relatively less lipid droplet deposition.

Serum from patients with E-PE, PE-HELLP, and APS with elevated levels of FFA had different effects on trophoblast cells, including cell morphology, lipid droplets deposition, and mitochondrial ultrastructure, suggesting disorders of lipid metabolism and fatty acid oxidation of various degrees and types. Serum from patients with E-PE led to damage similar to that of long-chain FFA in cell morphology, lipid droplet deposition and mitochondrial ultrastructure, indicating the correlation between disorders of long-chain fatty acid oxidation and the development of the disease.

语种英语
所属项目编号81241021 ; 7132215
资助者National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
WOS记录号WOS:000359380100017
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56644
Collection北京大学第三临床医学院
作者单位1.Peking Univ, Dept Obstet & Gynecol, Hosp 3, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Yu, Huan,Yang, Zi,Ding, Xiaoyan,et al. Effects of serum from patients with early-onset pre-eclampsia, HELLP syndrome, and antiphospholipid syndrome on fatty acid oxidation in trophoblast cells[J]. ARCHIVES OF GYNECOLOGY AND OBSTETRICS,2015,292(3):559-567.
APA Yu, Huan,Yang, Zi,Ding, Xiaoyan,Wang, Yanling,&Han, Yiwei.(2015).Effects of serum from patients with early-onset pre-eclampsia, HELLP syndrome, and antiphospholipid syndrome on fatty acid oxidation in trophoblast cells.ARCHIVES OF GYNECOLOGY AND OBSTETRICS,292(3),559-567.
MLA Yu, Huan,et al."Effects of serum from patients with early-onset pre-eclampsia, HELLP syndrome, and antiphospholipid syndrome on fatty acid oxidation in trophoblast cells".ARCHIVES OF GYNECOLOGY AND OBSTETRICS 292.3(2015):559-567.
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