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学科主题: 临床医学
题名:
Effects of serum from patients with early-onset pre-eclampsia, HELLP syndrome, and antiphospholipid syndrome on fatty acid oxidation in trophoblast cells
作者: Yu, Huan1; Yang, Zi1; Ding, Xiaoyan1; Wang, Yanling2; Han, Yiwei1
关键词: Fatty acid ; Oxidation ; Trophoblast cells ; Pre-eclampsia ; HELLP syndrome
刊名: ARCHIVES OF GYNECOLOGY AND OBSTETRICS
发表日期: 2015-09-01
DOI: 10.1007/s00404-015-3669-2
卷: 292, 期:3, 页:559-567
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology
研究领域[WOS]: Obstetrics & Gynecology
关键词[WOS]: LIPOPROTEIN
英文摘要:

The role of metabolic disorders of long-chain fatty acid oxidation in the development of pre-eclampsia (PE), hemolysis, elevated liver enzymes, and low platelets (HELLP) syndrome and antiphospholipid syndrome (APS) is unclear. The aim of this study was to research the effects of three serum on fatty acid oxidation in trophoblast cells.

Primary human trophoblast cells and HTR8/SVneo cells were treated with serum from patients with early-onset severe PE (E-PE), E-PE with HELLP (PE-HELLP), APS, and normal pregnant women as controls (NC). Cells treated with free fatty acids (FFAs) of various lengths were used as controls.

Triglyceride (TG) and FFA levels of the E-PE and APS groups were significantly higher than the PE-HELLP and NC groups (P < 0.05). Trophoblast cells treated with serum from the E-PE and APS groups showed obvious morphological changes and a large amount of lipid droplet deposition. Cells in the E-PE group had more severe damage of mitochondria ultrastructure, presenting with cell morphological changes, lipid droplet deposition, and mitochondria damage similar to the long-chain FFA group. FFA levels in the PE-HELLP group increased significantly compared with the NC group (P < 0.05), while TG levels did not change significantly (P > 0.05). Trophoblast cells treated with serum from the PE-HELLP group showed cellular morphology and mitochondria changes similar to the E-PE group, but had relatively less lipid droplet deposition.

Serum from patients with E-PE, PE-HELLP, and APS with elevated levels of FFA had different effects on trophoblast cells, including cell morphology, lipid droplets deposition, and mitochondrial ultrastructure, suggesting disorders of lipid metabolism and fatty acid oxidation of various degrees and types. Serum from patients with E-PE led to damage similar to that of long-chain FFA in cell morphology, lipid droplet deposition and mitochondrial ultrastructure, indicating the correlation between disorders of long-chain fatty acid oxidation and the development of the disease.

语种: 英语
所属项目编号: 81241021 ; 7132215
项目资助者: National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
WOS记录号: WOS:000359380100017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56644
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Obstet & Gynecol, Hosp 3, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China

Recommended Citation:
Yu, Huan,Yang, Zi,Ding, Xiaoyan,et al. Effects of serum from patients with early-onset pre-eclampsia, HELLP syndrome, and antiphospholipid syndrome on fatty acid oxidation in trophoblast cells[J]. ARCHIVES OF GYNECOLOGY AND OBSTETRICS,2015,292(3):559-567.
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