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学科主题: 临床医学
题名:
Establishment of plasma microRNA detection method by using taqman probe based quantitative reverse transcription PCR
作者: Wang, Y-N.1,2; Yu, L.; Zhao, X-S.3,4; Zhang, W.5; Wan, J.1,6; Yu, B.2,5
关键词: C. elegans microRNA (Cel-miR) ; MicroRNA-153 (miR-153) ; MicroRNA-486 (miR-486) ; Plasma microRNA ; Quantitative reverse transcription-PCR (qRT-PCR) ; Taqman probe
刊名: CELLULAR AND MOLECULAR BIOLOGY
发表日期: 2015
卷: 61, 期:3, 页:51-56
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: CIRCULATING MICRORNAS ; BREAST-CANCER ; HOST-DISEASE ; SERUM ; BIOMARKERS ; CELLS ; MICROARRAY ; DIAGNOSIS ; MIRNAS ; RNAS
英文摘要:

MicroRNAs (miRNAs) are a kind of short non-coding RNAs that regulate gene expression at the post-transcriptional level. Recently, many studies have found that circulating miRNAs have the potential to sever as diagnostic biomarkers for many diseases. However, the methods for the quantification of circulating miRNAs still need more adjustment. In this study, we tried to establish a reliable method to quantify the plasma miRNAs. We used quantitative real-time PCR with taqman probes to detect the plasma miR-153 level. Three controls were used in this study, including two external miRNAs control from C. elegans miRNAs (cel-miR-54 and cel-miR-238) and one internal control (hsa-miR-486). All of these controls were stable in plasma and the cel-miR-238/cel-miR-54/hsa-miR-486 combination could improve the normalization process. The expression level of the target miRNA, human plasma miR-153, could be quantified accurately with taqman probes. The assay has high accuracy, high sensitivity and a large dynamic range from 100 copies to 1013 copies in the PCR reaction. Our study provided a standardized quantification method for plasma miRNAs which might be used as biomarker in many diseases research.

语种: 英语
所属项目编号: 2014CB910204 ; 81000465 ; 81171017 ; 81271755 ; 81300440 ; 81371737 ; S2013010015545 ; 2014A030313708 ; GJHZ20120616153140827 ; JCYJ20130329110752142 ; JCYJ20120616153316095 ; KQC201105300001A ; KQCX20120803145850990 ; 7132181
项目资助者: National Basic Research Program of China (973 Program) ; National Natural Scientific Foundation of China ; Guangdong Natural Scientific Foundation ; Shenzhen Basic Research Grant ; Shenzhen Peacock Plan Research Grants ; Beijing Natural Science Foundation
WOS记录号: WOS:000358368600011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56649
Appears in Collections:北京大学深圳医院_皮肤性病科_期刊论文

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作者单位: 1.Hong Kong Univ Sci & Technol, Shenzhen Peking Univ, Shenzhen Key Lab Neuronal Struct Biol, Biomed Res Inst,Med Ctr, Shenzhen 518036, Guangdong, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Dermatol, Shenzhen 518036, Guangdong, Peoples R China
3.Peking Univ, Shenzhen Hosp, Dept Obstet & Gynaecol, Shenzhen 518036, Guangdong, Peoples R China
4.Peking Univ, Collaborat Innovat Ctr Hematol, Inst Hematol, Peoples Hosp, Beijing 100871, Peoples R China
5.Hong Kong Univ Sci & Technol, Shenzhen Peking Univ, Shenzhen Key Lab Translat Med Dermatol, Biomed Res Inst,Med Ctr, Shenzhen 518036, Guangdong, Peoples R China
6.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Wang, Y-N.,Yu, L.,Zhao, X-S.,et al. Establishment of plasma microRNA detection method by using taqman probe based quantitative reverse transcription PCR[J]. CELLULAR AND MOLECULAR BIOLOGY,2015,61(3):51-56.
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