IR@PKUHSC  > 北京大学药学院
学科主题药学
Functional expression of an earthworm fibrinolytic enzyme in Escherichia coli
Li, Da-Hui; Tong, Wei; Yang, Yan-fang
关键词Bacterial Expression Earthworm Fibrinolytic Lumbricus Rubellus Rt-pcr
刊名WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
2008-05-01
DOI10.1007/s11274-007-9515-3
24期:5页:613-618
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]LUMBRICUS-RUBELLUS ; MOLECULAR-CLONING ; PICHIA-PASTORIS ; EISENIA-FOETIDA ; SERINE-PROTEASE ; GENE
英文摘要

Two cDNA fragments (lrF1 and lrF2) representing a fibrinolytic enzyme gene of F-III-2 (GenBank AB045719), without and with signal peptide coding sequence, were cloned from earthworm Lumbricus rubellus. The two fragments were inserted into bacterial expression vector pET28a (+), respectively. Subsequent expression showed that both lrF1 and lrF2 proteins were produced as an inclusion body form in E. coli BL21 (DE3) pLysE. After protein refolding and purification, the fusion lrF1 and its derivative without poly histidine tags at the N-terminus showed fibrinolytic activity on fibrin plates with relative activity of 134.3 U/mg protein and 139.7 U/mg protein, respectively, whereas the fusion lrF2 and its derivative without the tags at the N-terminus, had no fibrinolytic activity. The results indicated that the E. coli expression system could not recognize the endogenous signal peptide of F-III-2, and the effect of the histidine tags at the N-terminus on the fibrinolytic activity of the expressed protein was insignificant.

语种英语
WOS记录号WOS:000254805700004
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56657
专题北京大学药学院
北京大学药学院_天然药物学系
作者单位Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Li, Da-Hui,Tong, Wei,Yang, Yan-fang. Functional expression of an earthworm fibrinolytic enzyme in Escherichia coli[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2008,24(5):613-618.
APA Li, Da-Hui,Tong, Wei,&Yang, Yan-fang.(2008).Functional expression of an earthworm fibrinolytic enzyme in Escherichia coli.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,24(5),613-618.
MLA Li, Da-Hui,et al."Functional expression of an earthworm fibrinolytic enzyme in Escherichia coli".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 24.5(2008):613-618.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Da-Hui]的文章
[Tong, Wei]的文章
[Yang, Yan-fang]的文章
百度学术
百度学术中相似的文章
[Li, Da-Hui]的文章
[Tong, Wei]的文章
[Yang, Yan-fang]的文章
必应学术
必应学术中相似的文章
[Li, Da-Hui]的文章
[Tong, Wei]的文章
[Yang, Yan-fang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。