北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Functional expression of an earthworm fibrinolytic enzyme in Escherichia coli
作者: Li, Da-Hui; Tong, Wei; Yang, Yan-fang
关键词: bacterial expression ; earthworm ; fibrinolytic ; Lumbricus rubellus ; RT-PCR
刊名: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
发表日期: 2008-05-01
DOI: 10.1007/s11274-007-9515-3
卷: 24, 期:5, 页:613-618
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
关键词[WOS]: LUMBRICUS-RUBELLUS ; MOLECULAR-CLONING ; PICHIA-PASTORIS ; EISENIA-FOETIDA ; SERINE-PROTEASE ; GENE
英文摘要:

Two cDNA fragments (lrF1 and lrF2) representing a fibrinolytic enzyme gene of F-III-2 (GenBank AB045719), without and with signal peptide coding sequence, were cloned from earthworm Lumbricus rubellus. The two fragments were inserted into bacterial expression vector pET28a (+), respectively. Subsequent expression showed that both lrF1 and lrF2 proteins were produced as an inclusion body form in E. coli BL21 (DE3) pLysE. After protein refolding and purification, the fusion lrF1 and its derivative without poly histidine tags at the N-terminus showed fibrinolytic activity on fibrin plates with relative activity of 134.3 U/mg protein and 139.7 U/mg protein, respectively, whereas the fusion lrF2 and its derivative without the tags at the N-terminus, had no fibrinolytic activity. The results indicated that the E. coli expression system could not recognize the endogenous signal peptide of F-III-2, and the effect of the histidine tags at the N-terminus on the fibrinolytic activity of the expressed protein was insignificant.

语种: 英语
WOS记录号: WOS:000254805700004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56657
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Li, Da-Hui,Tong, Wei,Yang, Yan-fang. Functional expression of an earthworm fibrinolytic enzyme in Escherichia coli[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2008,24(5):613-618.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Da-Hui]'s Articles
[Tong, Wei]'s Articles
[Yang, Yan-fang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Da-Hui]‘s Articles
[Tong, Wei]‘s Articles
[Yang, Yan-fang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace