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学科主题: 临床医学
题名:
Using immobilized G-protein coupled receptors to screen bioactive traditional Chinese medicine compounds with multiple targets
作者: Zhao, Xinfeng1,2; Li, Qian1; Bian, Liujiao1; Zheng, Xiaohui1,2; Zheng, Jianbin2; Zhang, Youyi3,4; Li, Zijian3,4
关键词: Adrenoceptors covalent immobilization ; Salvia miltiorrhiza ; Coptis chinensis ; Berberine ; Palmatine ; Jatrorrhizine
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2012-11-01
DOI: 10.1016/j.jpba.2012.05.004
卷: 70, 页:549-552
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: BINDING ; ALPHA(1)-ADRENOCEPTOR ; BETA(2)-ADRENOCEPTOR ; CHROMATOGRAPHY ; BERBERINE ; AFFINITY
英文摘要:

Demand on high-throughput methods for multi-target compounds screening continues to increase nowadays due to the decline of new drugs on the market. Two kinds of C-protein-coupled receptors, alpha1-adrenoceptor (alpha(1A)-AR) and beta2-adrenoceptor (beta(2)-AR), were purified and immobilized on the surface of macroporous silica gel to prepare new chromatographic stationary phases. Control drugs (e.g., prazosin, terazosin, salbutamol, and terbutaline) were used to characterize the retention behavior of the obtained alpha(1A)-AR and beta(2)-AR columns. This study also coupled both columns with a six-way switching valve to construct an automatic two-dimensional system for multi-target compounds screening in complex mixtures. Adrenaline hydrochloride was used as a representative drug to evaluate the chromatographic performance of the two dimensional system. The aqueous extracts from Salvia miltiorrhiza and Coptis chinensis were also analyzed by the automatic system. The compounds in S. miltiorrhiza had no binding to both alpha(1A)-AR and beta(2)-AR columns. But berberine, palmatine and jatrorrhizine were screened as the bioactive compounds in C. chinensis, targeting both the receptors. The proposed method is an alternative for recognizing and separating the compounds targeting different proteins from a complex matrix. (C) 2012 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 21005060 ; IRT1174 ; 20106101110001 ; 2008BAI51B01 ; 11JK0661
项目资助者: National Natural Science Foundation of China ; Chinese Ministry of Education ; National Science and Technology Support Plan Project ; Department of Education of Shaanxi Province
WOS记录号: WOS:000309314600071
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56661
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.NW Univ Xian, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Xian 710069, Peoples R China
2.NW Univ Xian, Inst Analyt Sci, Xian 710069, Peoples R China
3.Peking Univ, Inst Vasc Med, Hosp 3, Beijing 100083, Peoples R China
4.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China

Recommended Citation:
Zhao, Xinfeng,Li, Qian,Bian, Liujiao,et al. Using immobilized G-protein coupled receptors to screen bioactive traditional Chinese medicine compounds with multiple targets[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2012,70:549-552.
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