IR@PKUHSC  > 北京大学基础医学院  > 北京大学人类疾病基因研究中心
学科主题基础医学
Expression of immunoglobulin gene with classical V-(D)-J rearrangement in mouse brain neurons
Huang, Jing1; Sun, Xin1; Mao, Yuntao1; Zhu, Yiaohui1; Zhang, Pei1; Zhang, Li1; Du, Jin2; Qiu, Xiaoyan1
关键词immunoglobulin G expression gene rearrangement mouse neuron
刊名INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
2008
DOI10.1016/j.biocel.2007.12.004
40期:8页:1604-1615
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]GEL-ELECTROPHORESIS METHOD ; RECEPTOR MESSENGER-RNA ; CENTRAL-NERVOUS-SYSTEM ; CEREBRAL-CORTEX ; SUBPLATE ZONE ; HALF-ANTIBODY ; LIGHT-CHAIN ; CELLS ; PROTEIN ; QUANTIFICATION
英文摘要

Within the past 10 years, several investigators have reported the presence of immunoglobulin G in brain neurons. However, because immunoglobulin molecules were only known to be produced by B-lymphocytes, it was suspected that the neurons were taking immunoglobulin G up from the extracellular fluid. The aim of this study was to determine whether immunoglobulin G was actually being produced by the neurons. By immunohistochemistry and Western blotting analysis, we found that immunoglobulin G was also present in adult mouse brain neurons and isolated neonatal mouse neurons, respectively. More importantly, by in situ hybridization, Northern blotting and single cell reverse transcriptase polymerase chain reaction, the transcripts of rearranged immunoglobulin gamma chain and K chain were also found in adult mouse brain neurons. Further, confocal imaging of primary culture neurons showed that immunoglobulin G immunoreactivity was localized in the neuron cytoplasm, axons and dendrites. Immunoglobulin G extracted from the primary culture neurons could also be detected by Western blotting. Furthermore, the results of sulphur-35 or iodine-125 pulse-labeled immunoprecipitation provided additional confirmation that brain neurons could produce immunoglobulin G. Taken together, the results indicated that immunoglobulin G originated from both early generated and adult mouse neurons. Although the bioactivity of neuron-derived immunoglobulin G was not yet clear, we believed that immunoglobulin G might play an important role in neuronal development. (C) 2007 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000256536900021
Citation statistics
Cited Times:42[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56663
Collection北京大学基础医学院_北京大学人类疾病基因研究中心
作者单位1.Peking Univ, Inst Basic Med Sci, Ctr Human Dis Genom, Beijing 100083, Peoples R China
2.Peking Univ, Med Isotopes Res Ctr, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Huang, Jing,Sun, Xin,Mao, Yuntao,et al. Expression of immunoglobulin gene with classical V-(D)-J rearrangement in mouse brain neurons[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2008,40(8):1604-1615.
APA Huang, Jing.,Sun, Xin.,Mao, Yuntao.,Zhu, Yiaohui.,Zhang, Pei.,...&Qiu, Xiaoyan.(2008).Expression of immunoglobulin gene with classical V-(D)-J rearrangement in mouse brain neurons.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,40(8),1604-1615.
MLA Huang, Jing,et al."Expression of immunoglobulin gene with classical V-(D)-J rearrangement in mouse brain neurons".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 40.8(2008):1604-1615.
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