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学科主题: 口腔医学
题名:
BIOLOGICAL AND BACTERICIDAL PROPERTIES OF AG-DOPED BIOACTIVE GLASS IN A NATURAL EXTRACELLULAR MATRIX HYDROGEL WITH POTENTIAL APPLICATION IN DENTISTRY
作者: Wang, Y-Y.; Chatzistavrou, X.; Faulk, D.; Badylak, S.; Zheng, L.; Papagerakis, S.; Ge, L.; Liu, H.; Papagerakis, P.
关键词: Ag-doped bioactive glass ; natural extracellular matrix hydrogel ; dental pulp stem cells ; biological properties ; antibacterial activity
刊名: EUROPEAN CELLS & MATERIALS
发表日期: 2015
卷: 29, 页:342-355
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Cell Biology ; Engineering ; Materials Science
关键词[WOS]: PULP STEM-CELLS ; IN-VITRO ; TISSUE RECONSTRUCTION ; QUATERNARY AMMONIUM ; DENTAL APPLICATIONS ; SCAFFOLDS ; ANTIBACTERIAL ; REGENERATION ; FABRICATION ; COMPOSITES
英文摘要:

The aim of this study was the fabrication and evaluation of a novel bioactive and bactericidal material, which could have applications in dentistry by supporting tissue regeneration and killing oral bacteria. Our hypothesis was that a new scaffold for pulp-dentin tissue engineering with enhanced antibacterial activity could be obtained by associating extracellular matrix derived from porcine bladder with an antibacterial bioactive glass. Our study combines in vitro approaches and ectopic implantation in scid mice. The novel material was fabricated by incorporating a sol-gel derived silver (Ag)-doped bioactive glass (BG) in a natural extracellular matrix (ECM) hydrogel in ratio 1: 1 in weight % (Ag-BG/ECM). The biological properties of the Ag-BG/ECM were evaluated in culture with dental pulp stem cells (DPSCs). In particular, cell proliferation, cell apoptosis, stem cells markers profile, and cell differentiation potential were studied. Furthermore, the antibacterial activity against Streptococcus mutans and Lactobacillus casei was measured. Moreover, the capability of the material to enhance pulp/dentin regeneration in vivo was also evaluated. Our data show that Ag-BG/ECM significantly enhances DPSCs′ proliferation, it does not affect cell morphology and stem cells markers profile, protects cells from apoptosis, and enhances in vitro cell differentiation and mineralisation potential as well as in vivo dentin formation. Furthermore, Ag-BG/ECM strongly inhibits S. mutans and L. casei growth suggesting that the new material has also anti-bacterial properties. This study provides foundation for future clinical applications in dentistry. It could potentially advance the currently available options of dental regenerative materials.

语种: 英语
项目资助者: University of Michigan, School of Dentistry, Department of Orthodontics &amp ; Pediatric Dentistry ; University of Peking ; UM Department of Otolaryngology - Head and Neck Surgery
WOS记录号: WOS:000357101500022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56665
Appears in Collections:北京大学口腔医学院_儿童口腔科_期刊论文

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作者单位: 1.Univ Michigan, Sch Med, Dept Otolaryngol, Ann Arbor, MI 48109 USA
2.Univ Michigan, Sch Med, Ctr Organogenesis, Ann Arbor, MI 48109 USA
3.Univ Pittsburgh, McGowan Inst Regenerat Med, Pittsburgh, PA 15260 USA
4.Univ Pittsburgh, Dept Surg, Pittsburgh, PA 15260 USA
5.Univ Pittsburgh, Dept Bioengn, Pittsburgh, PA 15260 USA
6.Univ Michigan, Sch Dent, Dept Orthodont & Pediat Dent, Ann Arbor, MI 48109 USA
7.Peking Univ, Sch & Hosp Stomatol, Dept Pediat Dent, Beijing 100871, Peoples R China
8.Univ Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
9.Univ Michigan, Sch Med, Ctr Computat Med & Bioinformat, Ann Arbor, MI 48109 USA

Recommended Citation:
Wang, Y-Y.,Chatzistavrou, X.,Faulk, D.,et al. BIOLOGICAL AND BACTERICIDAL PROPERTIES OF AG-DOPED BIOACTIVE GLASS IN A NATURAL EXTRACELLULAR MATRIX HYDROGEL WITH POTENTIAL APPLICATION IN DENTISTRY[J]. EUROPEAN CELLS & MATERIALS,2015,29:342-355.
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