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学科主题: 临床医学
题名:
High-resolution melting analysis of PCDH10 methylation levels in gastric, colorectal and pancreatic cancers
作者: Yu, B.1,2; Yang, H.1,3; Zhang, C.1; Wu, Q.1; Shao, Y.1,2; Zhang, J.2; Guan, M.6; Wan, J.1,5; Zhang, W.4,5
关键词: PCDH10 ; methylation ; HRM ; gastric cancer ; colorectal cancer ; pancreatic cancer
刊名: NEOPLASMA
发表日期: 2010
DOI: 10.4149/neo_2010_03_247
卷: 57, 期:3, 页:247-252
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: DNA METHYLATION ; OL-PROTOCADHERIN ; TUMOR-SUPPRESSOR ; MOUSE-BRAIN ; CELL-LINES ; PCR BIAS ; EXPRESSION ; GENE ; SENSITIVITY ; MULTIPLE
英文摘要:

Protocadherins are cell-adhesion molecules with 6 or 7 cadherin motifs in their extracellular domain and various cyotoplasmic domains. PCDH10 was characterized a novel tumor suppressive gene in and was epigenetically silenced in multiple haematologic malignancies as well as some solid tumors such as gastric cancer, nasopharyngeal carcinoma and esophageal carcinoma. High-resolution melting (HRM) analysis has been used as a novel tool for analysis of promoter methylation. In our study, we used HRM analysis to detect the methylation levels of PCDH10 gene in 100 gastric cancers, 100 colorectal cancers, 70 pancreatic cancers and equal number of adjacent normal tissues. The frequency of PCDH10 methylation in all three types of cancers was significantly higher than that in normal tissues. Consistent with previous reports, expression levels of PCDH10 were inversely correlated with methylation levels. But we didn′t find significant association between PCDH10 methylation status and TNM staging in all three types of cancers. In summary, application of HRM analysis to large amount of clinical samples proves to be a fast and high-throughput way to investigate the epigenetic status of PCDH10. And this is the first study to evaluate the prevalence of PCDH10 boxed times methylation based on large amount of tumor samples, showing that epigenetic regulation of PCDH10 was associated with carcinogenesis.

语种: 英语
WOS记录号: WOS:000277412800009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56666
Appears in Collections:北京大学深圳医院_皮肤性病科_期刊论文

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作者单位: 1.Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen, Guangdong, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Dermatol, Shenzhen, Guangdong, Peoples R China
3.Peking Univ, Shenzhen Hosp, Dept Clin Lab, Shenzhen, Guangdong, Peoples R China
4.Ji Nan Univ, HKUST JNU Joint Lab, Jinan, Guangdong, Peoples R China
5.Hong Kong Univ Sci & Technol, Dept Biochem, Hong Kong, Hong Kong, Peoples R China
6.Fudan Univ, Huashan Hosp, Cent Lab, Dept Lab Med, Shanghai 200433, Peoples R China

Recommended Citation:
Yu, B.,Yang, H.,Zhang, C.,et al. High-resolution melting analysis of PCDH10 methylation levels in gastric, colorectal and pancreatic cancers[J]. NEOPLASMA,2010,57(3):247-252.
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