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学科主题: 药学
题名:
Biotransformation of 2 alpha,5 alpha,10 beta,14 beta-tetraacetoxy-4(20), 11-taxadiene by cell suspension cultures of Catharanthus roseus
作者: Dai, JU; Cui, J; Zhu, WH; Guo, HZ; Ye, M; Hu, Q; Zhang, DY; Zheng, JH; Guo, D
关键词: taxane ; biotransformation ; cell suspension cultures ; Catharanthus roseus ; Apocynaceae
刊名: PLANTA MEDICA
发表日期: 2002-12-01
卷: 68, 期:12, 页:1113-1117
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]: TUMOR-CELLS ; TAXININE
英文摘要:

Catharanthus roseus cell suspension cultures were employed for the biotransformation of 2alpha,5alpha,10beta,14beta-tetraacetoxy-4(20),11-taxadiene (1), and four metabolites were obtained. Based on their physical and chemical data, the structures of the four metabolites were respectively elucidated as 10beta-hydroxy-2alpha,5alpha,14beta-triacetoxy-4(20),11-taxadiene (2), 5alpha-hydroxy-2alpha,10beta,14beta-triacetoxy-4(20),11-taxadiene (3), 6alpha,10beta-dihydroxy-2alpha,5alpha,14beta-triacetoxy-4(20),11-taxadiene (4), and 6alpha,9alpha,10beta-trihydroxy-2alpha,5alpha 14beta-triacetoxy-4(20),11-taxadiene (5), among which 3 and 5 were characterized as new taxoids. The effects of the stages of substrate addition on the biotrans formation were also investigated. The results revealed that the biotransformation rate for 1 reached 85.3% and the yield of 2 70% when 1 was administered during the mid-linear phase (9 - 12(th) day) of the cell growth cycle. On the other hand, the yield for 4 reached the highest level of 11.8% when 1 was added in the early linear phase (6(th) day).

语种: 英语
WOS记录号: WOS:000180439100013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56670
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China
3.Peking Union Med Coll, Beijing 100050, Peoples R China

Recommended Citation:
Dai, JU,Cui, J,Zhu, WH,et al. Biotransformation of 2 alpha,5 alpha,10 beta,14 beta-tetraacetoxy-4(20), 11-taxadiene by cell suspension cultures of Catharanthus roseus[J]. PLANTA MEDICA,2002,68(12):1113-1117.
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