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学科主题: 临床医学
题名:
Adeno-associated virus-mediated Bcl-x(L) prevents aminoglycoside-induced hearing loss in mice
作者: Liu Yu-he; Ke Xiao-mei; Yong, Qin; Gu Zhi-ping; Xiao Shui-fang
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2007-07-20
卷: 120, 期:14, 页:1236-1240
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: HAIR CELL-DEATH ; BCL-X-L ; GENE-TRANSFER ; IN-VIVO ; COCHLEAR ; VECTOR ; FORM
英文摘要:

Background Recent studies showed that aminoglycosides destroyed the cochlear cells and induced ototoxicity by producing reactive oxygen species, including free radicals in the mitochondria, damaging the membrane of mitochondria and resulting in apoptotic cell death. Bcl-x(L) is a well characterized anti-apoptotic member of the Bcl-2 family. The aim of this study was to determine the potential cochlear protective effect of Bcl-x(L) as a therapeutic agent in the murine model of aminoglycoside ototoxicity.

Methods Serotype 2 of adeno-associated virus (AAV2) as a vector encoding the mouse Bcl-x(L) gene was injected into mice cochleae prior to injection of kanamycin. Bcl-x(L) expression in vitro and in vivo was examined with Western blotting and immunohistochemistry separately. Cochlear dissection and auditory steady state responses were checked to evaluate the cochlear structure and function.

Results The animals in the AAV2-Bcl-x(L)/kanamycin group displayed better auditory steady state responses hearing thresholds and cochlear structure than those in the artificial perilymph/kanamycin or AAV2-enhanced humanized green fluorescent protein/kanamycin control group at all tested frequencies. The auditory steady state responses hearing thresholds and cochlear structure in the inoculated side were better than that in the contralateral side.

Conclusions AAV2-Bcl-x(L) afforded significant preservation of the cochlear hair cells against ototoxic insults and protected the cochlear function. AAV2-mediated Bcl-x(L) might be an approach with respect to potential therapeutic application in the cochlear degeneration.

语种: 英语
WOS记录号: WOS:000248403900006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56672
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Otolaryngol Head & Neck Surg, Beijing 100034, Peoples R China

Recommended Citation:
Liu Yu-he,Ke Xiao-mei,Yong, Qin,et al. Adeno-associated virus-mediated Bcl-x(L) prevents aminoglycoside-induced hearing loss in mice[J]. CHINESE MEDICAL JOURNAL,2007,120(14):1236-1240.
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