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IR@PKUHSC  > 北京大学临床肿瘤学院  > 细胞生物学研究室  > 期刊论文
学科主题: 临床医学
题名:
High level expression and purification of recombinant PEX protein in cultured skeletal muscle cell expression system
作者: Song, Lin; Ke, Yang; Zhang, Zhi-Qian
关键词: QM7 ; expression system ; skeletal muscle ; PEX ; signal peptide ; MMP9
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2007-05-25
DOI: 10.1016/j.bbrc.2007.03.137
卷: 357, 期:1, 页:258-263
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: INSECT CELLS ; ANGIOGENESIS ; FRAGMENT
英文摘要:

Large quantities of recombinant proteins are needed for specific therapeutic and diagnostic applications. To achieve high-level expression in eukaryotic cells, the choice of cell line as well as the expression vector is critical. In this report, we demonstrate that a combination of the skeletal muscle cell line, QM7 and a cytomegalovirus promoter-based expression vector can achieve high-level expression of secretory recombinant proteins in eukaryotic cells. We also screened a serum-free medium containing 3 mu g/ml insulin suitable for QM7 differentiation and identified a very potent signal peptide from MMP9, which effectively directs secretion of heterologous proteins. The C-terminal hemopexin-like domain of MMP-2, PEX, a powerful candidate for the treatment of diseases associated with neovascularization was expressed in QM7 cells with bioactivity. This skeletal muscle cell-based system may be employed for the production of human proteins of special interests, such as those for structural determination or therapeutical development. (c) 2007 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000246028800042
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56682
Appears in Collections:北京大学临床肿瘤学院_细胞生物学研究室_期刊论文

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作者单位: Peking Univ, Sch Oncol, Dept Cell Biol, Beijing Canc Hosp & Inst, Beijing 100036, Peoples R China

Recommended Citation:
Song, Lin,Ke, Yang,Zhang, Zhi-Qian. High level expression and purification of recombinant PEX protein in cultured skeletal muscle cell expression system[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,357(1):258-263.
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