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学科主题基础医学
Mass Spectrometric Analysis of Phosphorylation Modification in 14-3-3 epsilon Protein
Li Zi-Jian1,2; He Xin3; Pan Chen-Yu4; Liu Ning4
关键词Mass Spectrometry 14-3-3 Epsilon Protein Phosphorylation
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2013-11-01
DOI10.1016/S1872-2040(13)60694-5
41期:11页:1653-1658
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]CELL-CYCLE ; INTEGRATION ; EXPRESSION ; APOPTOSIS ; CLONING ; DEATH
英文摘要

In the present study, 14-3-3 epsilon protein was over-expressed in HEK293 cells, which was genetically attached with 6 x His tag. The protein was purified and enriched by nickel-charged resin and gel electrophoresis, and then was subject to tryptic digestion. The phosphorylated peptide fragments within 14-3-3 epsilon protein were enriched by TiO2 affinity chromatography, followed by mass spectrometry analysis. Totally twelve phosphorylation sites along the primary structure of 14-3-3 epsilon protein were identified, most of which had not been reported previously. The results indicated that bio-mass spectrometry coupled with manual interpretation was quite feasible to identify the phosphorylation modification in 14-3-3 epsilon protein over-expressed in HEK293 cells.

语种英语
WOS记录号WOS:000327479300001
项目编号2011CB503900 ; 81070078 ; 21175055 ; 81270157 ; 7102158 ; 20110739 ; 2012210
资助机构Major State Basic Research Development Program of China (973 Program) ; Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation of China ; Jilin Province Science and Technology Department of China ; Jilin University Bethune Project B, China
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56705
专题北京大学基础医学院_心血管所
作者单位1.Jilin Univ, Hosp 2, Changchun 130041, Peoples R China
2.Jilin Univ, Dept Chem, Changchun 130021, Peoples R China
3.Peking Univ, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Key Lab Mol Cardiovasc Sci, Inst Vasc Med,Minist Hlth,Minist Educ,Hosp 3, Beijing 100191, Peoples R China
4.Beijing Key Lab Cardiovasc Receptors Res, Beijing 100191, Peoples R China
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GB/T 7714
Li Zi-Jian,He Xin,Pan Chen-Yu,et al. Mass Spectrometric Analysis of Phosphorylation Modification in 14-3-3 epsilon Protein[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2013,41(11):1653-1658.
APA Li Zi-Jian,He Xin,Pan Chen-Yu,&Liu Ning.(2013).Mass Spectrometric Analysis of Phosphorylation Modification in 14-3-3 epsilon Protein.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,41(11),1653-1658.
MLA Li Zi-Jian,et al."Mass Spectrometric Analysis of Phosphorylation Modification in 14-3-3 epsilon Protein".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 41.11(2013):1653-1658.
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