北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学深圳医院  > 期刊论文
学科主题: 临床医学
题名:
Interleukin-37 is increased in ankylosing spondylitis patients and associated with disease activity
作者: Chen, Bingni1,2,3; Huang, Kunzhao1,2,3; Ye, Liang1,2,3; Li, Yanqun1,2,3; Zhang, Jiawei1,2,3; Zhang, Jinshun1,2,3; Fan, Xinmin4; Liu, Xiaokai1,2,3; Li, Li1,2,3; Sun, Jinxia1,2,3; Du, Jing5; Huang, Zhong1,2,3
关键词: Interleukin-37 ; Ankylosing spondylitis ; Peripheral blood mononuclear cells ; Tumor necrosis factor-alpha ; Interleukin-17 ; Interleukin-6 ; Interleukin-23
刊名: JOURNAL OF TRANSLATIONAL MEDICINE
发表日期: 2015-01-28
DOI: 10.1186/s12967-015-0394-3
卷: 13
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: IL-1 FAMILY ; TNF-ALPHA ; CELLS ; OSTEOPOROSIS ; INFLAMMATION ; EXPRESSION ; IL-1-BETA ; PROTECTS ; INJURY ; MICE
英文摘要:

Background: Interleukin-37 (IL-37) has been known to play an immunosuppressive role in various inflammatory disorders, but whether it participates in the regulation of pathogenesis of ankylosing spondylitis (AS) has not been investigated. Here, we examined the serum levels of IL-37 and its clinical association in AS, and explored the anti-inflammatory effects of IL-37 on peripheral blood mononuclear cells (PBMCs) from AS patients.

Methods: The mRNA levels of IL-37, TNF-alpha, IL-6, IL-17, and IL-23 in PBMCs and their serum concentrations from 46 AS patients were examined by real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunoassay (ELISA), respectively. The correlations between serum IL-37 levels with disease activity, laboratory values and pro-inflammatory cytokines in AS were analyzed by Spearman correlation test. PBMCs from 46 AS patients were stimulated with recombinant IL-37 protein, expressions of TNF-alpha, IL-6, IL-17 and IL-23 were determined by RT-PCR and ELISA.

Results: Compared to healthy controls (HC), AS patients and active AS patients showed higher levels of IL-37 in PBMCs and serum respectively. Strikingly, serum IL-37 levels were higher in AS patients with osteoporosis than those without. Serum levels of IL-37 were correlated with laboratory values as well as TNF-alpha, IL-6 and IL-17, but not IL-23 in patients with AS. The productions of pro-inflammatory cytokines such as TNF-alpha, IL-6, IL-17, IL-23 in PBMCs from AS patients were obviously attenuated after recombinant IL-37 stimulation, but not in the HC.

Conclusion: The higher levels of IL-37 were found in AS patients, which were correlated with disease activity and AS related pro-inflammatory cytokines. More importantly, IL-37 inhibits the expressions of the pro-inflammatory cytokines from PBMCs in AS patients, indicating the potential anti-inflammatory role of IL-37 in AS.

语种: 英语
所属项目编号: 81273305 ; JCYJ20140711144858545
项目资助者: National Natural Science Foundation of China ; Special fund for Shenzhen strategic emerging industry development
WOS记录号: WOS:000350506000001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56720
Appears in Collections:北京大学深圳医院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Shenzhen Univ, Sch Med, Biol Therapy Inst, Shenzhen 518060, Guangdong, Peoples R China
2.Shenzhen Univ, Sch Med, Dept Pathogen Biol & Immunol, Shenzhen 518060, Guangdong, Peoples R China
3.Shenzhen City Shenzhen Univ, Immunodiagnost Technol Platforms, Shenzhen 518060, Peoples R China
4.Shenzhen Univ, Sch Med, Dept Pathol, Shenzhen 518060, Guangdong, Peoples R China
5.Peking Univ, Shenzhen Hosp, Dept Lab Med, Shenzhen 518036, Guangdong, Peoples R China

Recommended Citation:
Chen, Bingni,Huang, Kunzhao,Ye, Liang,et al. Interleukin-37 is increased in ankylosing spondylitis patients and associated with disease activity[J]. JOURNAL OF TRANSLATIONAL MEDICINE,2015,13.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen, Bingni]'s Articles
[Huang, Kunzhao]'s Articles
[Ye, Liang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen, Bingni]‘s Articles
[Huang, Kunzhao]‘s Articles
[Ye, Liang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace