北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner
作者: Zhao, Chuanke1; She, Tiantian1; Wang, Lixin1; Su, Yahui1; Qu, Like1; Gao, Yujing2; Xu, Shuo3; Cai, Shaoqing3; Shou, Chengchao1
关键词: Daucosterol ; Autophagy ; ROS ; Cancer
刊名: LIFE SCIENCES
发表日期: 2015-09-15
DOI: 10.1016/j.lfs.2015.07.019
卷: 137, 页:37-43
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: BETA-SITOSTEROL ; NATURAL-PRODUCTS ; DRUG DISCOVERY ; JNK ACTIVATION ; APOPTOSIS ; GROWTH ; THERAPY ; RESVERATROL ; NARINGENIN ; GENERATION
英文摘要:

Aims: This study aims to evaluate the anti-cancer effect of daucosterol and explore its possible mechanism.

Main methods: MTT and colony formation assay were performed to determine the effect of daucosterol on cancer cell proliferation in vitro. H22 allograft model was used for the assessment of its anti-cancer activity in vivo. Intracellular generation of reactive oxygen species (ROS) was measured using DCFH-DA probe with flow cytometry system and a laser scanning confocalmicroscope. LC3 (microtubule-associated protein 1 light chain 3)-II conversion was monitored with immunofluorescence and immunoblotting to demonstrate daucosterol-induced autophagy.

Key findings: We found that daucosterol inhibits the proliferation of human breast cancer cell line MCF-7 and gastric cancer cell lines MGC803, BGC823 and AGS in a dose-dependent manner. Furthermore, daucosterol inhibits murine hepatoma H22 cell growth in ICR mice. Daucosterol treatment induces intracellular ROS generation and autophagy, but not apoptotic cell death. Treatment with ROS scavenger GSH (reduced glutathione), NAC (N-acetyl-L-cysteine) or autophagy inhibitor 3-Methyladenine (3-MA) counteracted daucosterol-induced autophagy and growth inhibition in BGC823 and MCF-7 cancer cells.

Significance: Daucosterol inhibits cancer cell proliferation by inducing autophagy through ROS-dependent manner and could be potentially developed as an anti-cancer agent. (C) 2015 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 81301966 ; 2013-5
项目资助者: National Natural Science Foundation of China ; Peking University Cancer Hospital Institute
WOS记录号: WOS:000361035800006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56722
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ Canc Hosp & Inst, Dept Biochem & Mol Biol, Key Lab Carcinogenesis & Translat Res, Minist Educ, Beijing 100142, Peoples R China
2.Ningxia Med Univ, Dept Biochem & Mol Biol, Key Lab Fertil Preservat & Maintenance, Minist Educ, Yinchuan, Ningxia, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China

Recommended Citation:
Zhao, Chuanke,She, Tiantian,Wang, Lixin,et al. Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner[J]. LIFE SCIENCES,2015,137:37-43.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Chuanke]'s Articles
[She, Tiantian]'s Articles
[Wang, Lixin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Chuanke]‘s Articles
[She, Tiantian]‘s Articles
[Wang, Lixin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace