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Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner
Zhao, Chuanke1; She, Tiantian1; Wang, Lixin1; Su, Yahui1; Qu, Like1; Gao, Yujing2; Xu, Shuo3; Cai, Shaoqing3; Shou, Chengchao1
关键词Daucosterol Autophagy Ros Cancer
刊名LIFE SCIENCES
2015-09-15
DOI10.1016/j.lfs.2015.07.019
137页:37-43
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]BETA-SITOSTEROL ; NATURAL-PRODUCTS ; DRUG DISCOVERY ; JNK ACTIVATION ; APOPTOSIS ; GROWTH ; THERAPY ; RESVERATROL ; NARINGENIN ; GENERATION
英文摘要

Aims: This study aims to evaluate the anti-cancer effect of daucosterol and explore its possible mechanism.

Main methods: MTT and colony formation assay were performed to determine the effect of daucosterol on cancer cell proliferation in vitro. H22 allograft model was used for the assessment of its anti-cancer activity in vivo. Intracellular generation of reactive oxygen species (ROS) was measured using DCFH-DA probe with flow cytometry system and a laser scanning confocalmicroscope. LC3 (microtubule-associated protein 1 light chain 3)-II conversion was monitored with immunofluorescence and immunoblotting to demonstrate daucosterol-induced autophagy.

Key findings: We found that daucosterol inhibits the proliferation of human breast cancer cell line MCF-7 and gastric cancer cell lines MGC803, BGC823 and AGS in a dose-dependent manner. Furthermore, daucosterol inhibits murine hepatoma H22 cell growth in ICR mice. Daucosterol treatment induces intracellular ROS generation and autophagy, but not apoptotic cell death. Treatment with ROS scavenger GSH (reduced glutathione), NAC (N-acetyl-L-cysteine) or autophagy inhibitor 3-Methyladenine (3-MA) counteracted daucosterol-induced autophagy and growth inhibition in BGC823 and MCF-7 cancer cells.

Significance: Daucosterol inhibits cancer cell proliferation by inducing autophagy through ROS-dependent manner and could be potentially developed as an anti-cancer agent. (C) 2015 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000361035800006
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56722
专题北京大学药学院
北京大学药学院_天然药物学系
北京大学临床肿瘤学院_生化与分子生物学研究室
作者单位1.Peking Univ Canc Hosp & Inst, Dept Biochem & Mol Biol, Key Lab Carcinogenesis & Translat Res, Minist Educ, Beijing 100142, Peoples R China
2.Ningxia Med Univ, Dept Biochem & Mol Biol, Key Lab Fertil Preservat & Maintenance, Minist Educ, Yinchuan, Ningxia, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
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GB/T 7714
Zhao, Chuanke,She, Tiantian,Wang, Lixin,et al. Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner[J]. LIFE SCIENCES,2015,137:37-43.
APA Zhao, Chuanke.,She, Tiantian.,Wang, Lixin.,Su, Yahui.,Qu, Like.,...&Shou, Chengchao.(2015).Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner.LIFE SCIENCES,137,37-43.
MLA Zhao, Chuanke,et al."Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner".LIFE SCIENCES 137(2015):37-43.
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