IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
In Vitro Antioxidant and In Vivo Anti-Inflammatory Activities of Ophioglossum thermale
Zhang, Xue-Qiong1; Kim, Jin-Hwa2; Lee, Geun-Soo2; Pyo, Hyeong-Bae2; Shin, Eun-Young3; Kim, Eung-Gook3; Zhang, Yong-He1
关键词Ophioglossum Thermale Anti-inflammation Antioxidant Radical Scavenging Activity
刊名AMERICAN JOURNAL OF CHINESE MEDICINE
2012
DOI10.1142/S0192415X1250022X
40期:2页:279-293
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Integrative & Complementary Medicine ; Medicine, General & Internal
研究领域[WOS]Integrative & Complementary Medicine ; General & Internal Medicine
关键词[WOS]RADICAL-SCAVENGING ACTIVITY ; NITRIC-OXIDE ; FLAVONOIDS ; EXTRACTS ; DISEASE ; CELLS ; INFLAMMATION ; STRESS ; PLANT ; SKIN
英文摘要

Antioxidant fractions from Ophioglossum thermale were extracted with five different polar solvents using a Soxhlet type extractor. The total phenolic content of the extracts was determined by the Folin-Ciocalteu method. The ethyl acetate fraction of O. thermale was found to contain maximum phenolics. The dried fractions were screened for their antioxidant activity potential using in vitro model systems such as 1,1-diphenyl-2-picryl hydrazyl (DPPH), nitroblue tetrazolium (NBT) and lipid-peroxidation reduction at different concentrations. Results revealed that the EtOAc fraction exhibited the best performance in the DPPH assay, NBT assay and lipid peroxidation. All fractions showed more potent antioxidant capacity than green tea extract, a well-known antioxidant. Furthermore, the EtOAc fraction has the highest total phenolic content (475.65 mg of EGCG/g). In addition, the EtOAc fraction at 0.005% and 0.01% (g/100 ml) also significantly inhibited UVB irradiation-induced ROS generation in human dermal fibroblasts (HDFs). In a carrageenan-induced edema model, the EtOAc fraction showed an inhibitory effect (21.5%, p < 0: 05) at 200 mg/kg (p.o.) after 300 min administration. Consequently, 3-O-methylquercetin (3MQ) was also isolated from the antioxidative EtOAc fraction. The data obtained using the above in vitro and in vivo tests suggest that the antioxidant activity of O. thermale and its anti-inflammatory effect on carrageenan-induced acute inflammation can be attributed to its ameliorating effect on oxidative damage, and thus it has great potential as a source for natural health products. To the best of our knowledge, this is the first report on the antioxidant activity of different polar extracts from O. thermale.

语种英语
WOS记录号WOS:000301504700006
项目编号70004326 ; 2009ZX09103-124 ; 30640070 ; 30772556 ; 20100001110048
资助机构Ministry of Knowledge Economy (MKE) of Korea ; National Scientific Technological ; National Natural Science Foundation of China ; Ministry of Education of China ; Peking University
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56743
专题北京大学基础医学院_药理学系
北京大学基础医学院
作者单位1.Peking Univ, Dept Pharmacol, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Hanbul Cosmet Co Ltd, R&D Ctr, Chungbuk 369830, South Korea
3.Chungbuk Natl Univ, Coll Med, Dept Biochem, Chonju 361763, South Korea
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GB/T 7714
Zhang, Xue-Qiong,Kim, Jin-Hwa,Lee, Geun-Soo,et al. In Vitro Antioxidant and In Vivo Anti-Inflammatory Activities of Ophioglossum thermale[J]. AMERICAN JOURNAL OF CHINESE MEDICINE,2012,40(2):279-293.
APA Zhang, Xue-Qiong.,Kim, Jin-Hwa.,Lee, Geun-Soo.,Pyo, Hyeong-Bae.,Shin, Eun-Young.,...&Zhang, Yong-He.(2012).In Vitro Antioxidant and In Vivo Anti-Inflammatory Activities of Ophioglossum thermale.AMERICAN JOURNAL OF CHINESE MEDICINE,40(2),279-293.
MLA Zhang, Xue-Qiong,et al."In Vitro Antioxidant and In Vivo Anti-Inflammatory Activities of Ophioglossum thermale".AMERICAN JOURNAL OF CHINESE MEDICINE 40.2(2012):279-293.
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