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学科主题: 临床医学
题名:
The construction of recombinant adenovirus P37 and its function of promoting migration of BICR
作者: Gong Man-Man; Meng Lin; Shou Cheng-Chao
关键词: adenovirus ; mycoplasma ; P37 ; cancer ; migration
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2007-11-01
卷: 34, 期:11, 页:1147-1151
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: MYCOPLASMA-HYORHINIS ; PROTEIN ; SYSTEM ; CELLS
英文摘要:

Previous work showed that there was high ratio of Mycoplasma hyorhinis infection in human gastric carcinoma. P37 protein is a membrane lipoprotein of Mycoplasma hyorhinis. It was reported that P37 inhibited cell adhesion and induced tumor invasiveness. To investigate the function of P37 in cancer development, the p37 gene was subcloned into shuttle vector of pAdTrack-CMV. Linearized pAdTrack-CMV-p37 was co-transformed into BJ5183 cells with adenoviral genomic plasmid pAdEasy-1. The identified recombinant DNA was transfected into 293 cells to package adenovirus. From the supernatant and cell lysis, the presence of recombinant adenovirus P37 was proved by PCR. And then BICR cells with this recombinant adenovirus of P37 were successfully infected. RT-PCR and Western blot demonstrated the transcription and expression of P37 in infected BICR cells. And the soluble P37 protein can be secreted into the culture supernatant of infected BICR cells. In the migration assay in vitro, the number of migration BICR cells infected with Ad-p37 was 52.03% more than that of control. The construction of recombinant adenovirus provided a good tool for studying P37 function and its molecular mechanism, which will be further used for in vivo migration and invasion experiments.

语种: 中文
WOS记录号: WOS:000251022800005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56751
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Beijing 100036, Peoples R China

Recommended Citation:
Gong Man-Man,Meng Lin,Shou Cheng-Chao. The construction of recombinant adenovirus P37 and its function of promoting migration of BICR[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2007,34(11):1147-1151.
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