IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
The construction of recombinant adenovirus P37 and its function of promoting migration of BICR
Gong Man-Man; Meng Lin; Shou Cheng-Chao
关键词Adenovirus Mycoplasma P37 Cancer Migration
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2007-11-01
34期:11页:1147-1151
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]MYCOPLASMA-HYORHINIS ; PROTEIN ; SYSTEM ; CELLS
英文摘要

Previous work showed that there was high ratio of Mycoplasma hyorhinis infection in human gastric carcinoma. P37 protein is a membrane lipoprotein of Mycoplasma hyorhinis. It was reported that P37 inhibited cell adhesion and induced tumor invasiveness. To investigate the function of P37 in cancer development, the p37 gene was subcloned into shuttle vector of pAdTrack-CMV. Linearized pAdTrack-CMV-p37 was co-transformed into BJ5183 cells with adenoviral genomic plasmid pAdEasy-1. The identified recombinant DNA was transfected into 293 cells to package adenovirus. From the supernatant and cell lysis, the presence of recombinant adenovirus P37 was proved by PCR. And then BICR cells with this recombinant adenovirus of P37 were successfully infected. RT-PCR and Western blot demonstrated the transcription and expression of P37 in infected BICR cells. And the soluble P37 protein can be secreted into the culture supernatant of infected BICR cells. In the migration assay in vitro, the number of migration BICR cells infected with Ad-p37 was 52.03% more than that of control. The construction of recombinant adenovirus provided a good tool for studying P37 function and its molecular mechanism, which will be further used for in vivo migration and invasion experiments.

语种中文
WOS记录号WOS:000251022800005
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56751
专题北京大学临床肿瘤学院
作者单位Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Beijing 100036, Peoples R China
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GB/T 7714
Gong Man-Man,Meng Lin,Shou Cheng-Chao. The construction of recombinant adenovirus P37 and its function of promoting migration of BICR[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2007,34(11):1147-1151.
APA Gong Man-Man,Meng Lin,&Shou Cheng-Chao.(2007).The construction of recombinant adenovirus P37 and its function of promoting migration of BICR.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,34(11),1147-1151.
MLA Gong Man-Man,et al."The construction of recombinant adenovirus P37 and its function of promoting migration of BICR".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 34.11(2007):1147-1151.
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