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Gene delivery into primary cerebral cortical neurons by lentiviral vector
Zhang, Yuzhi; Wang, Hansen; Pan, Hong; Bao, Xinhua; Li, Meirong; Jin, Jing; Wu, Xiru
关键词Cortical Neurons Transduction Lentiviral Vectors Gene Transfer
刊名CELL BIOLOGY INTERNATIONAL
2006-10-01
DOI10.1016/j.cellbi.2006.05.010
30期:10页:777-783
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]CENTRAL-NERVOUS-SYSTEM ; HIPPOCAMPAL-NEURONS ; NONDIVIDING CELLS ; EFFICIENT ; MODEL ; TRANSDUCTION ; PROTEIN ; VIRUS ; EXPRESSION ; DISEASE
英文摘要

Several studies have shown the ability of human immunodeficiency virus type 1 (HIV-1)-based lentiviral vectors to infect nondividing brain neurons. We are the first to show that primary embryonic cerebral cortical neurons can be efficiently transduced by an HIV-1-based lentiviral vector encoding enhanced green fluorescent protein (EGFP). We also describe the optimal conditions for the transduction of cerebral cortical neurons with lentiviral Vectors, and the kinetic process of infection. The percentage of cells expressing EGFP is a function of the time in culture and virus dose. The highest percentage of EGFP-expression achieved was 46.77% at 4 days in vitro (DIV) with a multiplicity of infection (m.o.i.) of 20. The results show that lentiviral vectors are not only good prospects for in vivo gene delivery, but are also good candidates for in vitro studies of the function of gene products in primary cerebral cortical neurons, (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000241794900003
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56764
专题北京大学第一临床医学院_儿科
作者单位Peking Univ, Dept Pediat, Hosp 1, Beijing 100034, Peoples R China
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Zhang, Yuzhi,Wang, Hansen,Pan, Hong,et al. Gene delivery into primary cerebral cortical neurons by lentiviral vector[J]. CELL BIOLOGY INTERNATIONAL,2006,30(10):777-783.
APA Zhang, Yuzhi.,Wang, Hansen.,Pan, Hong.,Bao, Xinhua.,Li, Meirong.,...&Wu, Xiru.(2006).Gene delivery into primary cerebral cortical neurons by lentiviral vector.CELL BIOLOGY INTERNATIONAL,30(10),777-783.
MLA Zhang, Yuzhi,et al."Gene delivery into primary cerebral cortical neurons by lentiviral vector".CELL BIOLOGY INTERNATIONAL 30.10(2006):777-783.
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