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学科主题: 临床医学
题名:
Gene delivery into primary cerebral cortical neurons by lentiviral vector
作者: Zhang, Yuzhi; Wang, Hansen; Pan, Hong; Bao, Xinhua; Li, Meirong; Jin, Jing; Wu, Xiru
关键词: cortical neurons ; transduction ; lentiviral vectors ; gene transfer
刊名: CELL BIOLOGY INTERNATIONAL
发表日期: 2006-10-01
DOI: 10.1016/j.cellbi.2006.05.010
卷: 30, 期:10, 页:777-783
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: CENTRAL-NERVOUS-SYSTEM ; HIPPOCAMPAL-NEURONS ; NONDIVIDING CELLS ; EFFICIENT ; MODEL ; TRANSDUCTION ; PROTEIN ; VIRUS ; EXPRESSION ; DISEASE
英文摘要:

Several studies have shown the ability of human immunodeficiency virus type 1 (HIV-1)-based lentiviral vectors to infect nondividing brain neurons. We are the first to show that primary embryonic cerebral cortical neurons can be efficiently transduced by an HIV-1-based lentiviral vector encoding enhanced green fluorescent protein (EGFP). We also describe the optimal conditions for the transduction of cerebral cortical neurons with lentiviral Vectors, and the kinetic process of infection. The percentage of cells expressing EGFP is a function of the time in culture and virus dose. The highest percentage of EGFP-expression achieved was 46.77% at 4 days in vitro (DIV) with a multiplicity of infection (m.o.i.) of 20. The results show that lentiviral vectors are not only good prospects for in vivo gene delivery, but are also good candidates for in vitro studies of the function of gene products in primary cerebral cortical neurons, (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000241794900003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56764
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: Peking Univ, Dept Pediat, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, Yuzhi,Wang, Hansen,Pan, Hong,et al. Gene delivery into primary cerebral cortical neurons by lentiviral vector[J]. CELL BIOLOGY INTERNATIONAL,2006,30(10):777-783.
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