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学科主题临床医学
The myocardial response to adrenomedullin involves increased cAMP generation as well as augmented Akt phosphorylation
Pan, Chun Shui; Jin, Shao Ju; Cao, Chang Qi; Zhao, Jing; Zhang, Jing; Wang, Xian; Tang, Chao Shu; Qi, Yong Fen
关键词Isoproterenol Myocardial Ischemic Injury Adrenomedullin Calcitonin Receptor-like Receptor Receptor Activity Modifying Proteins Akt Phosphorylation
刊名PEPTIDES
2007-04-01
DOI10.1016/j.peptides.2007.01.011
28期:4页:900-909
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]PHOSPHATIDYLINOSITOL 3-KINASE/AKT-DEPENDENT PATHWAY ; RAT-HEART ; ENDOGENOUS ADRENOMEDULLIN ; GENE-EXPRESSION ; RECEPTOR SYSTEM ; ISOPROTERENOL ; CELLS ; ISCHEMIA ; HYPOXIA ; PEPTIDE
英文摘要

in this work we aimed to observe (1) the changes in adrenomedullin (AM) and its receptor system - calcitonin receptor-like receptor (CRLR) and receptor activity modifying proteins (RAMPs) - in myocardial ischemic injury and (2) the response of injuried myocardia to AM and the phosphorylation. of Akt to illustrate the protective mechanism of AM in ischemic myocardia. Male SD rats were subcutaneously injected with isoproterenol (ISO) to induce myocardial ischemia. The mRNA levels of AM, CRLR, RAMP1, RAMP2 and RAMP3 were determined by RT-PCR. Protein levels of Akt, phosphor-Akt, CRLR, RAMP1, RAMP2 and RAMP3 were assayed by Western blot. Results showed that, compared with that of the controls, ISO-treated rats showed lower cardiac function and myocardial injury. The mRNA relative amount of AM, CRLR, RAMP1, RAMP2 and RAMP3 in the myocardia of ISO-treated rats was increased. The elevated mRNA levels of CRLR, RAMP1, RAMP2 and RAMP3 were positively correlated with AM content in injured myocardia. The protein levels of CRLR, RAMP1, RAMP2 and RAMP3 in injured myocardia were increased compared with that of control myocardia. AM-stimulated cAMP generation in myocardia was elevated in the ISO group, and was antagonized by AM22-S2 and CGRP(8-37). Western blot analyses revealed that AM significantly enhanced Akt phosphorylation in injured myocardia, which was blocked by pretreatment with AM(22-52) or CGRP(8-37). Ischemia-injured myocardia hyper-expressed AM and its receptors - CRLR, RAMP1, RAMP2 and RAMP3 and the response of ischemic myocardia to AM was potentiated, and the level of Akt phosphorylation was also increased, which suggests that changes in cardiac AM/AM receptor might play an important role in the pathogenesis of myocardial ischemic injury, (c) 2007 Published by Elsevier Inc.

语种英语
WOS记录号WOS:000245625900023
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56766
专题北京大学第一临床医学院_心血管内科
作者单位1.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
3.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Pan, Chun Shui,Jin, Shao Ju,Cao, Chang Qi,et al. The myocardial response to adrenomedullin involves increased cAMP generation as well as augmented Akt phosphorylation[J]. PEPTIDES,2007,28(4):900-909.
APA Pan, Chun Shui.,Jin, Shao Ju.,Cao, Chang Qi.,Zhao, Jing.,Zhang, Jing.,...&Qi, Yong Fen.(2007).The myocardial response to adrenomedullin involves increased cAMP generation as well as augmented Akt phosphorylation.PEPTIDES,28(4),900-909.
MLA Pan, Chun Shui,et al."The myocardial response to adrenomedullin involves increased cAMP generation as well as augmented Akt phosphorylation".PEPTIDES 28.4(2007):900-909.
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