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Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry
Yao, Hongjun; Shi, Yujie; Gao, Rongfu; Zhang, Guifeng; Zhang, Rumin; Zheng, Caixia; Xu, Bingjiu
关键词Photosystem i Lipids Reversed-phase High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2006-06-06
DOI10.1016/j.jchromb.2006.04.011
837期:1-2页:101-107
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]QUANTITATIVE-ANALYSIS ; SEPARATION ; PHOSPHATIDYLCHOLINE ; PHOSPHOLIPIDS ; EXTRACTS ; SYSTEM ; FIELD ; MS
英文摘要

A method for simultaneous analysis of lipids extracted from photosystem I complex was developed with high performance liquid chromatography/electrospray ionization mass spectrometry. The photosystem I complex was firstly solubilized and separated using deoxycholate polyacrylamide gel electrophoresis method after ultrasonic treatment of the sample (leaves of pea, Pisum sativum L.). The Photosystem I complexes were electrophoretically eluted from the deoxycholate polyacrylamide gel electrophoresis bands containing them, and the electron transport activity of the eluent measured as confirmation. Lipids, which were isolated from the complex having photosystem I activity, were separated and characterized with high performance liquid chromatography/electrospray ionization mass spectrometry. Five lipids, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylglycerol, sulphoquinovosyldiacylglycerol and phosphaditylcholine were found combining with photosystem I complex. Different species of these lipids were found in the ESI mass spectra and the compositions of the acyl groups in them were determined. (c) 2006 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000238311600014
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56780
专题北京大学药学院
作者单位1.Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Minist Educ, Beijing 100083, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Beijing 100080, Peoples R China
4.Capital Univ Med Sci, Sch Chem Biol & Pharm Sci, Dept Anal Chem, Beijing 100054, Peoples R China
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GB/T 7714
Yao, Hongjun,Shi, Yujie,Gao, Rongfu,et al. Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,837(1-2):101-107.
APA Yao, Hongjun.,Shi, Yujie.,Gao, Rongfu.,Zhang, Guifeng.,Zhang, Rumin.,...&Xu, Bingjiu.(2006).Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,837(1-2),101-107.
MLA Yao, Hongjun,et al."Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 837.1-2(2006):101-107.
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