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学科主题: 药学
题名:
Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry
作者: Yao, Hongjun; Shi, Yujie; Gao, Rongfu; Zhang, Guifeng; Zhang, Rumin; Zheng, Caixia; Xu, Bingjiu
关键词: photosystem I ; lipids ; reversed-phase high performance liquid chromatography ; electrospray ionization mass spectrometry
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2006-06-06
DOI: 10.1016/j.jchromb.2006.04.011
卷: 837, 期:1-2, 页:101-107
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: QUANTITATIVE-ANALYSIS ; SEPARATION ; PHOSPHATIDYLCHOLINE ; PHOSPHOLIPIDS ; EXTRACTS ; SYSTEM ; FIELD ; MS
英文摘要:

A method for simultaneous analysis of lipids extracted from photosystem I complex was developed with high performance liquid chromatography/electrospray ionization mass spectrometry. The photosystem I complex was firstly solubilized and separated using deoxycholate polyacrylamide gel electrophoresis method after ultrasonic treatment of the sample (leaves of pea, Pisum sativum L.). The Photosystem I complexes were electrophoretically eluted from the deoxycholate polyacrylamide gel electrophoresis bands containing them, and the electron transport activity of the eluent measured as confirmation. Lipids, which were isolated from the complex having photosystem I activity, were separated and characterized with high performance liquid chromatography/electrospray ionization mass spectrometry. Five lipids, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylglycerol, sulphoquinovosyldiacylglycerol and phosphaditylcholine were found combining with photosystem I complex. Different species of these lipids were found in the ESI mass spectra and the compositions of the acyl groups in them were determined. (c) 2006 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000238311600014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56780
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Minist Educ, Beijing 100083, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Beijing 100080, Peoples R China
4.Capital Univ Med Sci, Sch Chem Biol & Pharm Sci, Dept Anal Chem, Beijing 100054, Peoples R China

Recommended Citation:
Yao, Hongjun,Shi, Yujie,Gao, Rongfu,et al. Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,837(1-2):101-107.
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