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学科主题: 临床医学
题名:
Aspirin increases susceptibility of Helicobacter pylori to metronidazole by augmenting endocellular concentrations of antimicrobials
作者: Zhang, Xiao-Ping; Wang, Wei-Hong; Tian, Yu; Gao, Wen; Li, Jiang
关键词: Helicobacter pylori ; Aspirin ; Metronidazole ; Resistance ; Minimum inhibitory concentrations
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2009-02-28
DOI: 10.3748/wjg.15.919
卷: 15, 期:8, 页:919-926
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: NONSTEROIDAL ANTIINFLAMMATORY DRUGS ; ESCHERICHIA-COLI ; KLEBSIELLA-PNEUMONIAE ; STAPHYLOCOCCUS-EPIDERMIDIS ; SODIUM-SALICYLATE ; RESISTANCE ; INFECTION ; EFFLUX ; EXPRESSION ; ACID
英文摘要:

AIM: To investigate the mechanisms of aspirin increasing the susceptibility of Helicobacter pylori (H pylori) to metronidazole.

METHODS: H pylori reference strain 26 695 and two metronidazole-resistant isolates of H pylori were included in this study. Strains were incubated in Brucella broth with or without aspirin (1 mmol/L). The rdxA gene of H pylori was amplified by PCR and sequenced. The permeability of H pylori to antimicrobials was determined by analyzing the endocellular radioactivity of the cells after incubated with [7-(3)H]-tetracycline. The outer membrane proteins (OMPs) of H pylori 26695 were depurated and analyzed by SDS-PAGE. The expression of 5 porins (hopA, hopB, hopC, hopD and hopE) and the putative RND efflux system (hefABC) of H pylori were analyzed using real-time quantitative PCR.

RESULTS: The mutations in rdxA gene did not change in metronidazole resistant isolates treated with aspirin. The radioactivity of H pylori increased when treated with aspirin, indicating that aspirin improved the permeability of the outer membrane of H pylori. However, the expression of two OMP bands between 55 kDa and 72 kDa altered in the presence of aspirin. The expression of the mRNA of hopA, hopB, hopC, hopD, hopE and hefA, hefB, hefC of H pylori did not change when treated with aspirin.

CONCLUSION: Although aspirin increases the susceptibility of H pylori to metronidazole, it has no effect on the mutations of rdxA gene of H pylori. Aspirin increases endocellular concentrations of antimicrobials probably by altering the OMP expression. (C) 2009 The WJG Press and Baishideng. All rights reserved.

语种: 英语
所属项目编号: 30470777
项目资助者: The National Natural Science Foundation of China
WOS记录号: WOS:000263927100004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56783
Appears in Collections:北京大学第一临床医学院_消化内科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Gastroenterol, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, Xiao-Ping,Wang, Wei-Hong,Tian, Yu,et al. Aspirin increases susceptibility of Helicobacter pylori to metronidazole by augmenting endocellular concentrations of antimicrobials[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2009,15(8):919-926.
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