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学科主题临床医学
Aspirin increases susceptibility of Helicobacter pylori to metronidazole by augmenting endocellular concentrations of antimicrobials
Zhang, Xiao-Ping; Wang, Wei-Hong; Tian, Yu; Gao, Wen; Li, Jiang
关键词Helicobacter Pylori Aspirin Metronidazole Resistance Minimum Inhibitory Concentrations
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2009-02-28
DOI10.3748/wjg.15.919
15期:8页:919-926
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]NONSTEROIDAL ANTIINFLAMMATORY DRUGS ; ESCHERICHIA-COLI ; KLEBSIELLA-PNEUMONIAE ; STAPHYLOCOCCUS-EPIDERMIDIS ; SODIUM-SALICYLATE ; RESISTANCE ; INFECTION ; EFFLUX ; EXPRESSION ; ACID
英文摘要

AIM: To investigate the mechanisms of aspirin increasing the susceptibility of Helicobacter pylori (H pylori) to metronidazole.

METHODS: H pylori reference strain 26 695 and two metronidazole-resistant isolates of H pylori were included in this study. Strains were incubated in Brucella broth with or without aspirin (1 mmol/L). The rdxA gene of H pylori was amplified by PCR and sequenced. The permeability of H pylori to antimicrobials was determined by analyzing the endocellular radioactivity of the cells after incubated with [7-(3)H]-tetracycline. The outer membrane proteins (OMPs) of H pylori 26695 were depurated and analyzed by SDS-PAGE. The expression of 5 porins (hopA, hopB, hopC, hopD and hopE) and the putative RND efflux system (hefABC) of H pylori were analyzed using real-time quantitative PCR.

RESULTS: The mutations in rdxA gene did not change in metronidazole resistant isolates treated with aspirin. The radioactivity of H pylori increased when treated with aspirin, indicating that aspirin improved the permeability of the outer membrane of H pylori. However, the expression of two OMP bands between 55 kDa and 72 kDa altered in the presence of aspirin. The expression of the mRNA of hopA, hopB, hopC, hopD, hopE and hefA, hefB, hefC of H pylori did not change when treated with aspirin.

CONCLUSION: Although aspirin increases the susceptibility of H pylori to metronidazole, it has no effect on the mutations of rdxA gene of H pylori. Aspirin increases endocellular concentrations of antimicrobials probably by altering the OMP expression. (C) 2009 The WJG Press and Baishideng. All rights reserved.

语种英语
WOS记录号WOS:000263927100004
项目编号30470777
资助机构The National Natural Science Foundation of China
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56783
专题北京大学口腔医学院_儿童口腔科
北京大学第一临床医学院_消化科
作者单位Peking Univ, Hosp 1, Dept Gastroenterol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Xiao-Ping,Wang, Wei-Hong,Tian, Yu,et al. Aspirin increases susceptibility of Helicobacter pylori to metronidazole by augmenting endocellular concentrations of antimicrobials[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2009,15(8):919-926.
APA Zhang, Xiao-Ping,Wang, Wei-Hong,Tian, Yu,Gao, Wen,&Li, Jiang.(2009).Aspirin increases susceptibility of Helicobacter pylori to metronidazole by augmenting endocellular concentrations of antimicrobials.WORLD JOURNAL OF GASTROENTEROLOGY,15(8),919-926.
MLA Zhang, Xiao-Ping,et al."Aspirin increases susceptibility of Helicobacter pylori to metronidazole by augmenting endocellular concentrations of antimicrobials".WORLD JOURNAL OF GASTROENTEROLOGY 15.8(2009):919-926.
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