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Differential proteomic analysis of the anti-depressive effects of oleamide in a rat chronic mild stress model of depression
Ge, Lin1; Zhu, Ming-ming1; Yang, Jing-Yu1; Wang, Fang1; Zhang, Rong2; Zhang, Jing-Hai2; Shen, Jing3; Tian, Hui-Fang3; Wu, Chun-Fu1
关键词Chronic Mild Stress Hippocampus Oleamide Proteomics
刊名PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
2015-04-01
DOI10.1016/j.pbb.2015.01.017
131页:77-86
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Behavioral Sciences ; Neurosciences ; Pharmacology & Pharmacy
研究领域[WOS]Behavioral Sciences ; Neurosciences & Neurology ; Pharmacology & Pharmacy
关键词[WOS]SUMO-2 TARGET PROTEINS ; ACTIVE LIPID OLEAMIDE ; DIPHOSPHATE KINASE-B ; C-DELTA INTERACTION ; FATTY-ACID AMIDES ; ADENYLATE KINASE ; PREFRONTAL CORTEX ; IN-VITRO ; SUMO2/3-CONJUGATED PROTEINS ; QUANTITATIVE PROTEOMICS
英文摘要

Depression is a complex psychiatric disorder, and its etiology and pathophysiology are not completely understood. Depression involves changes in many biogenic amine, neuropeptide, and oxidative systems, as well as alterations in neuroendocrine function and immune-inflammatory pathways. Oleamide is a fatty amide which exhibits pharmacological effects leading to hypnosis, sedation, and anti-anxiety effects. In the present study, the chronic mild stress (CMS) model was used to investigate the antidepressant-like activity of oleamide. Rats were exposed to 10 weeks of CMS or control conditions and were then subsequently treated with 2 weeks of daily oleamide (5 mg/kg, i.p.), fluoxetine (10 mg/kg, i.p.), or vehicle. Protein extracts from the hippocampus were then collected, and hippocampal maps were generated by way of two-dimensional gel electrophoresis (2-DE). Altered proteins induced by CMS and oleamide were identified through mass spectrometry and database searches. Compared to the control group, the CMS rats exhibited significantly less body weight gain and decreased sucrose consumption. Treatment with oleamide caused a reversal of the CMS-induced deficit in sucrose consumption. In the proteomic analysis, 12 protein spots were selected and identified. CMS increased the levels of adenylate kinase isoenzyme 1 (AK1), nucleoside diphosphate kinase B (NDKB), histidine triad nucleotide-binding protein 1 (HINT1), acyl-protein thioesterase 2 (APT-2), and glutathione S-transferase A4 (GSTA4). Compared to the CMS samples, seven spots changed significantly following treatment with oleamide, including GSTA4, glutathione S-transferase A6 (GSTA6), GTP-binding nuclear protein Ran (Ran-GTP), ATP synthase subunit d, transgelin-3, small ubiquitin-related modifier 2 (SUMO2), and eukaryotic translation initiation factor 5A-1 (elF5A1). Of these seven proteins, the level of elF5A1 was up-regulated, whereas the remaining proteins were down-regulated. In conclusion, oleamide has antidepressant-like properties in the CMS rat model. The identification of proteins altered by CMS and oleamide treatment provides support for targeting these proteins in the development of novel therapies for depression. (C) 2015 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000352173200013
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56797
专题北京大学临床肿瘤学院_中心实验室
作者单位1.Shenyang Pharmaceut Univ, Dept Pharmacol, Shenyang 110016, Peoples R China
2.Shenyang Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Shenyang 110015, Peoples R China
3.Beijing Canc Hosp & Inst, Cent Lab, Minist Educ, Key Lab Carcinogenesis & Translat Res, Beijing 100142, Peoples R China
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Ge, Lin,Zhu, Ming-ming,Yang, Jing-Yu,et al. Differential proteomic analysis of the anti-depressive effects of oleamide in a rat chronic mild stress model of depression[J]. PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR,2015,131:77-86.
APA Ge, Lin.,Zhu, Ming-ming.,Yang, Jing-Yu.,Wang, Fang.,Zhang, Rong.,...&Wu, Chun-Fu.(2015).Differential proteomic analysis of the anti-depressive effects of oleamide in a rat chronic mild stress model of depression.PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR,131,77-86.
MLA Ge, Lin,et al."Differential proteomic analysis of the anti-depressive effects of oleamide in a rat chronic mild stress model of depression".PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR 131(2015):77-86.
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