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学科主题临床医学
Diffusion of macromolecule through retina after experimental branch retinal vein occlusion and estimate of intraretinal barrier
Tao, Yong; Li, Xiao-xin; Jiang, Yan-rong; Bai, Xia-bing; Wu, Bi-dong; Dong, Jian-qiang
关键词Retinal Vein Occlusion Drug Diffusion Macromolecule Intraretinal Diffusion Barrier Intravitreous Drug Delivery
刊名CURRENT DRUG METABOLISM
2007-02-01
8期:2页:151-156
收录类别SCI
文章类型Review
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]TISSUE-PLASMINOGEN ACTIVATOR ; EXPERIMENTAL SUBRETINAL HEMORRHAGE ; INTRAVITREAL BEVACIZUMAB AVASTIN ; MACULAR DEGENERATION ; MOLECULAR-WEIGHT ; MORPHOMETRIC-ANALYSIS ; PIG MODEL ; AGE ; PHARMACOKINETICS ; PIGMENTOSA
英文摘要

The disposition and diffusion knowledge of intravitreally injected macromolecule drugs through retina in pathological condition is crucial but the related studies are absent. Retinal edema is a common pathological change of fundus diseases and retinal vein occlusion (RVO) pig model were established to emulate it. FITC-dextrans of various molecular weights were dissolved in RPMI-1640 solutions and the rate of transretinal diffusion was determined with a spectrophotometer. Theoretical maximum size of molecule (MSM) was calculated by extrapolating the trend-linear relationship with the diffusion rate. In separate experiments to determine the sites of barrier to diffusion, FITC-dextrans were applied to either the inner or outer retinal surface, processed as frozen sections, and viewed with a fluorescence microscope. Paired-Samples T test was used to compared the diffusion rate of dextrans of the both eyes of one pig. The MSM in RVO tissues and normal tissue was 6.5 +/- 0.39nm and 6.18 +/- 0.54nm respectively (t=4.143, P=0.0001). FITC-dextrans applying to inner retinal surface, 4.4 kDa dextran were largely arrested at inner nuclear layer (INL). The INL of the 19.6 similar to 71.2 kDa dextran diffusion retina section became dark and the nerve fiber layer (NFL) and inner plexiform layer got brighter. As for 150 kDa dextran, the NFL was bright and the other layers were dark. FITC-dextrans applying to outer retinal surface, most dextrans were blocked before outer nuclear layer (ONL). In summary, ONL and INL may act as bottle-neck barriers to diffusion of macromolecules. Compared with normal neuroretina, the MSM of fresh edema retina after RVO increased limitedly.

语种英语
WOS记录号WOS:000244702800004
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56808
专题北京大学第二临床医学院_眼科
作者单位1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Beijing Univ Aeronaut & Astronaut, Sch Mat Sci & Engn, Beijing, Peoples R China
3.Peking Univ, Peoples Hosp, Pathol Ctr Lab, Beijing 100044, Peoples R China
4.Peking Univ, Peoples Hosp, Beijing Inst Pharmacol & Toxicol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Tao, Yong,Li, Xiao-xin,Jiang, Yan-rong,et al. Diffusion of macromolecule through retina after experimental branch retinal vein occlusion and estimate of intraretinal barrier[J]. CURRENT DRUG METABOLISM,2007,8(2):151-156.
APA Tao, Yong,Li, Xiao-xin,Jiang, Yan-rong,Bai, Xia-bing,Wu, Bi-dong,&Dong, Jian-qiang.(2007).Diffusion of macromolecule through retina after experimental branch retinal vein occlusion and estimate of intraretinal barrier.CURRENT DRUG METABOLISM,8(2),151-156.
MLA Tao, Yong,et al."Diffusion of macromolecule through retina after experimental branch retinal vein occlusion and estimate of intraretinal barrier".CURRENT DRUG METABOLISM 8.2(2007):151-156.
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