北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 眼科  > 期刊论文
学科主题: 临床医学
题名:
Diffusion of macromolecule through retina after experimental branch retinal vein occlusion and estimate of intraretinal barrier
作者: Tao, Yong; Li, Xiao-xin; Jiang, Yan-rong; Bai, Xia-bing; Wu, Bi-dong; Dong, Jian-qiang
关键词: retinal vein occlusion ; drug diffusion ; macromolecule ; intraretinal diffusion barrier ; intravitreous drug delivery
刊名: CURRENT DRUG METABOLISM
发表日期: 2007-02-01
卷: 8, 期:2, 页:151-156
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: TISSUE-PLASMINOGEN ACTIVATOR ; EXPERIMENTAL SUBRETINAL HEMORRHAGE ; INTRAVITREAL BEVACIZUMAB AVASTIN ; MACULAR DEGENERATION ; MOLECULAR-WEIGHT ; MORPHOMETRIC-ANALYSIS ; PIG MODEL ; AGE ; PHARMACOKINETICS ; PIGMENTOSA
英文摘要:

The disposition and diffusion knowledge of intravitreally injected macromolecule drugs through retina in pathological condition is crucial but the related studies are absent. Retinal edema is a common pathological change of fundus diseases and retinal vein occlusion (RVO) pig model were established to emulate it. FITC-dextrans of various molecular weights were dissolved in RPMI-1640 solutions and the rate of transretinal diffusion was determined with a spectrophotometer. Theoretical maximum size of molecule (MSM) was calculated by extrapolating the trend-linear relationship with the diffusion rate. In separate experiments to determine the sites of barrier to diffusion, FITC-dextrans were applied to either the inner or outer retinal surface, processed as frozen sections, and viewed with a fluorescence microscope. Paired-Samples T test was used to compared the diffusion rate of dextrans of the both eyes of one pig. The MSM in RVO tissues and normal tissue was 6.5 +/- 0.39nm and 6.18 +/- 0.54nm respectively (t=4.143, P=0.0001). FITC-dextrans applying to inner retinal surface, 4.4 kDa dextran were largely arrested at inner nuclear layer (INL). The INL of the 19.6 similar to 71.2 kDa dextran diffusion retina section became dark and the nerve fiber layer (NFL) and inner plexiform layer got brighter. As for 150 kDa dextran, the NFL was bright and the other layers were dark. FITC-dextrans applying to outer retinal surface, most dextrans were blocked before outer nuclear layer (ONL). In summary, ONL and INL may act as bottle-neck barriers to diffusion of macromolecules. Compared with normal neuroretina, the MSM of fresh edema retina after RVO increased limitedly.

语种: 英语
WOS记录号: WOS:000244702800004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56808
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Beijing Univ Aeronaut & Astronaut, Sch Mat Sci & Engn, Beijing, Peoples R China
3.Peking Univ, Peoples Hosp, Pathol Ctr Lab, Beijing 100044, Peoples R China
4.Peking Univ, Peoples Hosp, Beijing Inst Pharmacol & Toxicol, Beijing 100044, Peoples R China

Recommended Citation:
Tao, Yong,Li, Xiao-xin,Jiang, Yan-rong,et al. Diffusion of macromolecule through retina after experimental branch retinal vein occlusion and estimate of intraretinal barrier[J]. CURRENT DRUG METABOLISM,2007,8(2):151-156.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Tao, Yong]'s Articles
[Li, Xiao-xin]'s Articles
[Jiang, Yan-rong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Tao, Yong]‘s Articles
[Li, Xiao-xin]‘s Articles
[Jiang, Yan-rong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!