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学科主题: 药学
题名:
N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection
作者: Wang, Xinyi1,2,3; Lin, Ruoyun2; Xu, Zhihan1; Huang, Hongduan2; Li, Limei3; Liu, Feng2; Li, Na2; Yang, Xiaoda1
关键词: Fluorescence ; Quenching ; Silver nanoclusters ; Nucleotide ; N-acetylcysteine
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2013-09-02
DOI: 10.1016/j.aca.2013.07.037
卷: 793, 页:79-85
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: ACETYL-L-CYSTEINE ; LABEL-FREE ; AG NANOCLUSTERS ; DNA ; ACID ; FLUOROPHORES ; DISULFIDES ; CLUSTERS ; PROTEIN ; THIOLS
英文摘要:

In this work, we reported a new, simple and sensitive method for determination of N-acetylcysteine (NAC) based on quenching of the red fluorescence of oligonuleotide-protected silver nanoculsters (Ag NCs) with the quantum yield of 68.3 +/- 0.3%. This method was successfully used for the assay of NAC granules presenting a linear range from 100 nM to 1200 nM (LOD of 50 nM) with minimal interferences from potential coexisting substances. It is for the first time that quenching performance of the thiol-containing compound was found to follow a non-linear Stern-Volmer profile, indicative of a complicated quenching mechanism with static quenching dominating, in which DNA-template of Ag NCs was partly replaced by NAC, as elucidated by spectral investigations. This study extended the analytical application of silver nanoclusters as well as provided a more insightful understanding of the quenching mechanism of thiol-compounds on the fluorescence of Ag NCs. (c) 2013 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 21035005 ; 20975004 ; 21275011 ; L2011108
项目资助者: National Natural Science Foundation of China ; Education Department of Liaoning Province
WOS记录号: WOS:000323855400010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56813
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Bioorgan Chem & Mol Engn,BNLMS, Beijing 100871, Peoples R China
3.Shenyang Agr Univ, Coll Sci, Shenyang 110161, Peoples R China

Recommended Citation:
Wang, Xinyi,Lin, Ruoyun,Xu, Zhihan,et al. N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection[J]. ANALYTICA CHIMICA ACTA,2013,793:79-85.
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