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N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection
Wang, Xinyi1,2,3; Lin, Ruoyun2; Xu, Zhihan1; Huang, Hongduan2; Li, Limei3; Liu, Feng2; Li, Na2; Yang, Xiaoda1
关键词Fluorescence Quenching Silver Nanoclusters Nucleotide N-acetylcysteine
刊名ANALYTICA CHIMICA ACTA
2013-09-02
DOI10.1016/j.aca.2013.07.037
793页:79-85
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]ACETYL-L-CYSTEINE ; LABEL-FREE ; AG NANOCLUSTERS ; DNA ; ACID ; FLUOROPHORES ; DISULFIDES ; CLUSTERS ; PROTEIN ; THIOLS
英文摘要

In this work, we reported a new, simple and sensitive method for determination of N-acetylcysteine (NAC) based on quenching of the red fluorescence of oligonuleotide-protected silver nanoculsters (Ag NCs) with the quantum yield of 68.3 +/- 0.3%. This method was successfully used for the assay of NAC granules presenting a linear range from 100 nM to 1200 nM (LOD of 50 nM) with minimal interferences from potential coexisting substances. It is for the first time that quenching performance of the thiol-containing compound was found to follow a non-linear Stern-Volmer profile, indicative of a complicated quenching mechanism with static quenching dominating, in which DNA-template of Ag NCs was partly replaced by NAC, as elucidated by spectral investigations. This study extended the analytical application of silver nanoclusters as well as provided a more insightful understanding of the quenching mechanism of thiol-compounds on the fluorescence of Ag NCs. (c) 2013 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000323855400010
项目编号21035005 ; 20975004 ; 21275011 ; L2011108
资助机构National Natural Science Foundation of China ; Education Department of Liaoning Province
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56813
专题北京大学医学部管理机构_医学部
北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Bioorgan Chem & Mol Engn,BNLMS, Beijing 100871, Peoples R China
3.Shenyang Agr Univ, Coll Sci, Shenyang 110161, Peoples R China
推荐引用方式
GB/T 7714
Wang, Xinyi,Lin, Ruoyun,Xu, Zhihan,et al. N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection[J]. ANALYTICA CHIMICA ACTA,2013,793:79-85.
APA Wang, Xinyi.,Lin, Ruoyun.,Xu, Zhihan.,Huang, Hongduan.,Li, Limei.,...&Yang, Xiaoda.(2013).N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection.ANALYTICA CHIMICA ACTA,793,79-85.
MLA Wang, Xinyi,et al."N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection".ANALYTICA CHIMICA ACTA 793(2013):79-85.
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