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学科主题临床医学
Expression of ECRG4, a novel esophageal cancer-related gene, downregulated by CpG island hypermethylation in human esophageal squamous cell carcinoma
Yue, CM; Deng, DJ; Bi, MX; Guo, LP; Lu, SH
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2003-06-01
9期:6页:1174-1178
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]TUMOR-SUPPRESSOR GENES ; DNA METHYLATION ; LUNG-CANCER ; PROMOTER ; CARCINOGENESIS ; INACTIVATION ; PATTERNS
英文摘要

AIM: To study the mechanisms responsible for inactivation of a novel esophageal cancer related gene 4 (ECRG4) in esophageal squamous cell carcinoma (ESCC).

METHODS: A pair of primers was designed to amplify a 220 bp fragment, which contains 16 CpG sites in the core promoter region of the ECRG 4 gene. PCR products of bisulfite-modified CpG islands were analyzed by denaturing high-performance liquid chromatography (DHPLC), which were confirmed by DNA sequencing. The methylation status of ECRG 4 promoter in 20 cases of esophageal cancer and the adjacent normal tissues, 5 human tumor cell lines (esophageal cancer cell line-NEC, EC109, EC9706; gastric cancer cell line- GLC; human embryo kidney cell line-Hek293) and 2 normal esophagus tissues were detected. The expression level of the ECRG 4 gene in these samples was examined by RT-PCR.

RESULTS: The expression level of ECRG 4 gene was varied. Of 20 esophageal cancer tissues, nine were unexpressed, six were lowly expressed and five were highly expressed compared with the adjacent tissues and the 2 normal esophageal epithelia. In addition, 4 out of the 5 human cell lines were also unexpressed. A high frequency of methylation was revealed in 12 (8 unexpressed and 4 lowly expressed) of the 15 (80%) downregulated cancer tissues and 3 of the 4 unexpressed cell lines. No methylation peak was observed in the two highly expressed normal esophageal epithelia and the methylation frequency was low (3/20) among the 20 cases in the highly expressed adjacent tissues. The methylation status of the samples was consistent with the result of DNA sequencing.

CONCLUSION: These results indicate that the inactivation of ECRG 4 gene by hypermethylation is a frequent molecular event in ESCC and may be involved in the carcinogenesis of this cancer.

语种英语
WOS记录号WOS:000183763000007
引用统计
被引频次:53[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56824
专题北京大学临床肿瘤学院
作者单位1.Chinese Acad Med Sci, Dept Etiol & Carcinogenesis, Beijing 100021, Peoples R China
2.Peking Union Med Coll, Dept Etiol & Carcinogenesis, Beijing 100021, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing 100034, Peoples R China
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GB/T 7714
Yue, CM,Deng, DJ,Bi, MX,et al. Expression of ECRG4, a novel esophageal cancer-related gene, downregulated by CpG island hypermethylation in human esophageal squamous cell carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2003,9(6):1174-1178.
APA Yue, CM,Deng, DJ,Bi, MX,Guo, LP,&Lu, SH.(2003).Expression of ECRG4, a novel esophageal cancer-related gene, downregulated by CpG island hypermethylation in human esophageal squamous cell carcinoma.WORLD JOURNAL OF GASTROENTEROLOGY,9(6),1174-1178.
MLA Yue, CM,et al."Expression of ECRG4, a novel esophageal cancer-related gene, downregulated by CpG island hypermethylation in human esophageal squamous cell carcinoma".WORLD JOURNAL OF GASTROENTEROLOGY 9.6(2003):1174-1178.
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