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学科主题临床医学
Amplification of the miR-181c/d cluster is inversely correlated with PDCD4 expression in gastric cancer
Pan, Yuanming1,2; Xing, Rui1; An, Juan1,3; Cui, Jiantao1; Li, Wenmei1; Guo, Mingzhou2; Lu, Youyong1
关键词Mir-181c Mir-181d Pdcd4 Cnv Gastric Cancer
刊名CHINESE SCIENCE BULLETIN
2014-07-01
DOI10.1007/s11434-014-0280-z
59期:19页:2240-2248
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Basic Research Program ; National High Technology Research and Development Program ; National Basic Research Program ; National High Technology Research and Development Program
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]CHROMOSOMAL INSTABILITY ; TUMOR SUPPRESSORS ; OVARIAN-CARCINOMA ; TARGET GENES ; STEM-CELLS ; MICRORNAS ; ONCOGENES ; GENOME ; IDENTIFICATION ; TUMORIGENESIS
英文摘要

miR-181c/d is dysregulated in gastric cancer (GC). We investigated the amplification and expression of miR-181c/d and its predicted target genes in GC. Amplification of miR-181c/d was quantified by genomic real-time PCR in GC and adjacent normal tissues, as well as the levels of mature miR-181c/d was performed by real-time PCR in the same tissues. The potential target genes of miR-181c/d were predicted using bioinformatics software. Expression of one potential target gene, PDCD4, was measured by semi-quantitative RT-PCR, real-time PCR, and immunohistochemistry. Next, the relationship between miR181c/d expression and PDCD4 expression was analyzed. Results indicated that the amplification and expression of miR-181c/d were significantly higher in GC than in adjacent normal tissues (primary miR-181c/d, P < 0.001; miR-181c, P = 0.0344; miR-181d, P = 0.0153), and there was a strong correlation between mature miR-181c/d and primary miR-181c/d. Thirty-two target genes were predicted, including PDCD4 which is a known tumor suppressor gene. Expression of PDCD4 was significantly down-regulated in GC as compared to adjacent normal tissues and was inversely correlated with miR-181c/d expression in GC (miR-181c and PDCD4: R = -0.496, P = 0.008; miR-181d and PDCD4: R = -0.454, P = 0.003). Therefore, miR-181c/d may play a pivotal role in the pathogenesis of GC by down-regulating PDCD4 expression.

语种英语
所属项目编号2012CB934002 ; 2010CB912802 ; 2012AA02A504 ; SS2012AA02A209 ; SS2012AA020821 ; SS2012AA02A203
资助者National Basic Research Program ; National High Technology Research and Development Program ; National Basic Research Program ; National High Technology Research and Development Program
WOS记录号WOS:000337139000004
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56838
专题北京大学临床肿瘤学院
作者单位1.Peking Univ, Canc Hosp Inst, Minist Educ, Lab Mol Oncol,Key Lab Carcinogenesis & Translat R, Beijing 100142, Peoples R China
2.Chinese Peoples Liberat Army Gen Hosp, Dept Gastroenterol & Hepatol, Beijing 100853, Peoples R China
3.Qinghai Univ, Dept Basic Med Sci, High Altitude Med Res Ctr, Xining 810001, Peoples R China
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Pan, Yuanming,Xing, Rui,An, Juan,et al. Amplification of the miR-181c/d cluster is inversely correlated with PDCD4 expression in gastric cancer[J]. CHINESE SCIENCE BULLETIN,2014,59(19):2240-2248.
APA Pan, Yuanming.,Xing, Rui.,An, Juan.,Cui, Jiantao.,Li, Wenmei.,...&Lu, Youyong.(2014).Amplification of the miR-181c/d cluster is inversely correlated with PDCD4 expression in gastric cancer.CHINESE SCIENCE BULLETIN,59(19),2240-2248.
MLA Pan, Yuanming,et al."Amplification of the miR-181c/d cluster is inversely correlated with PDCD4 expression in gastric cancer".CHINESE SCIENCE BULLETIN 59.19(2014):2240-2248.
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