IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Purified human bone marrow multipotent mesenchymal stem cells regenerate infarcted myocardium in experimental rats
Zhang, SH; Jia, ZQ; Ge, JB; Gong, LZ; Ma, YL; Li, T; Guo, JX; Chen, P; Hu, QK; Zhang, P; Liu, YG; Li, ZP; Ma, KT; Li, LS; Zhou, CY
关键词Mesenchymal Stem Cell Transplantation Differentiation Myocardial Infarction Angiogenesis
刊名CELL TRANSPLANTATION
2005
14期:10页:787-798
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Medicine, Research & Experimental ; Transplantation
研究领域[WOS]Cell Biology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]STROMAL CELLS ; ISCHEMIC-MYOCARDIUM ; FUSION ; TRANSDIFFERENTIATION ; DIFFERENTIATION ; TRANSPLANTATION ; CARDIOMYOCYTES ; ENGRAFTMENT ; REPAIR ; BMI-1
英文摘要

Recent findings suggest the feasibility of cardiac repair by transplantation of bone marrow mesenchymal stem cell (MSCs). However, it remains controversial regarding which cell type is the best source for transplanting into the ischemic heart because of lack of well-defined cell markers. In this Study, we investigated the in vitro and in vivo effects of the novel multipotent marrow mesenchymal stein cells (MMSCs) from human bone marrow. Pluripotent markers (Oct4. Bmi1, and Abcg2) and vascular endothelial growth factor (VEGF) were detected by RT-PCR and immunofluorescence in MMSCs. Myocardial differentiation was induced in the expanded MMSC cultures by treatment with 5-azacyline. Expressions of VEGF in the animals transplanted with MMSCs were markedly increased in comparison with the animals injected with fibroblasts or saline at both mRNA and protein levels. VEGF expression was observed in both transplanted MMSCs and recipient cardiomyocytes by immunofluorescence. Confocal immunofluorescence microscopy revealed the specific markers for cardiomyocytes and endothelial cells in transplanted MMSCs 14 days after transplantation. Vessel count was increased and left ventricular function improved post-MMSC transplantation. These results indicate that transplantation of purified MMSCs from human bone marrow upregulated VEGF expression, enhanced angiogenesis, and improved the functional recovery following myocardial infarction in rats.

语种英语
WOS记录号WOS:000234907000008
引用统计
被引频次:45[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56842
专题北京大学基础医学院
作者单位1.Peking Univ, Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100083, Peoples R China
3.Fudan Univ, Shanghai Inst Cardiovasc Dis, Shanghai 20032, Peoples R China
4.Peking Univ, Dept Cardiol, Hosp 3, Beijing 10083, Peoples R China
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Zhang, SH,Jia, ZQ,Ge, JB,et al. Purified human bone marrow multipotent mesenchymal stem cells regenerate infarcted myocardium in experimental rats[J]. CELL TRANSPLANTATION,2005,14(10):787-798.
APA Zhang, SH.,Jia, ZQ.,Ge, JB.,Gong, LZ.,Ma, YL.,...&Zhou, CY.(2005).Purified human bone marrow multipotent mesenchymal stem cells regenerate infarcted myocardium in experimental rats.CELL TRANSPLANTATION,14(10),787-798.
MLA Zhang, SH,et al."Purified human bone marrow multipotent mesenchymal stem cells regenerate infarcted myocardium in experimental rats".CELL TRANSPLANTATION 14.10(2005):787-798.
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