IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1
Wang, Pingzhang1,2,3; Xiong, Ying1; Ma, Chuan1; Shi, Taiping1,2,3; Ma, Dalong1,2,3
关键词AP-1 Cloning JNK2 MAPK9 Transcript variant
刊名BMB REPORTS
2010-11-30
DOI10.3858/BMBRep.2010.43.11.738
43期:11页:738-743
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
资助者National High Technology Research and the Development Program of China ; Key National ST Program ; National High Technology Research and the Development Program of China ; Key National ST Program
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]SIGNAL-TRANSDUCTION ; KINASE ; AUTOPHOSPHORYLATION ; JNK2-ALPHA-2 ; REGULATOR ; ISOFORMS ; DOMAIN
英文摘要

The c-Jun NH2-terminal kinase (JNK) signaling pathway participates in many physiological functions. In the current study we reported the cloning and characterization of five novel JNK2 transcript variants, which were designated as INK2 alpha 3, JNK2 alpha 4, JNK2 beta 3, JNK2 gamma 1 and JNK2 gamma 2, respectively. Among them, JNK2 alpha 4 and JNK2 gamma 2 are potential non-coding RNA because they contain pre-mature stop codons. Both JNK2 alpha 3 and JNK2 beta 3 contain an intact kinase domain, and both encode a protein product of 46 kDa, the same as those of JNK2 alpha 1 and JNK2 beta 1. JNK2 gamma 1 contains a disrupted kinase domain and it showed a disable function. When over-expressed in mammalian cells, JNK2a3 showed higher activity on AP-1 than that of JNK2 beta 3 and JNK2 gamma 1. Furthermore, JNK2 alpha 3 and JNK2 beta 3 showed different levels of substrate phosphorylation, although they both could promote the proliferation of 293T cells. Our results further demonstrate that JNK2 isoforms preferentially target different substrates and may regulate the expression of various target genes. [BMB reports 2010; 43(11): 738-743]

语种英语
所属项目编号2006AA02A305 ; 2009ZX09503-004
资助者National High Technology Research and the Development Program of China ; Key National ST Program ; National High Technology Research and the Development Program of China ; Key National ST Program
WOS记录号WOS:000284974500005
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56848
Collection北京大学基础医学院_免疫学系
作者单位1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Lab Med Immunol, Beijing 100191, Peoples R China
3.Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Wang, Pingzhang,Xiong, Ying,Ma, Chuan,et al. Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1[J]. BMB REPORTS,2010,43(11):738-743.
APA Wang, Pingzhang,Xiong, Ying,Ma, Chuan,Shi, Taiping,&Ma, Dalong.(2010).Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1.BMB REPORTS,43(11),738-743.
MLA Wang, Pingzhang,et al."Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1".BMB REPORTS 43.11(2010):738-743.
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