IR@PKUHSC  > 基础医学院  > 免疫学系
学科主题基础医学
Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1
Wang, Pingzhang1,2,3; Xiong, Ying1; Ma, Chuan1; Shi, Taiping1,2,3; Ma, Dalong1,2,3
关键词AP-1 Cloning JNK2 MAPK9 Transcript variant
刊名BMB REPORTS
2010-11-30
DOI10.3858/BMBRep.2010.43.11.738
43期:11页:738-743
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
资助者National High Technology Research and the Development Program of China ; Key National ST Program ; National High Technology Research and the Development Program of China ; Key National ST Program
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]SIGNAL-TRANSDUCTION ; KINASE ; AUTOPHOSPHORYLATION ; JNK2-ALPHA-2 ; REGULATOR ; ISOFORMS ; DOMAIN
英文摘要

The c-Jun NH2-terminal kinase (JNK) signaling pathway participates in many physiological functions. In the current study we reported the cloning and characterization of five novel JNK2 transcript variants, which were designated as INK2 alpha 3, JNK2 alpha 4, JNK2 beta 3, JNK2 gamma 1 and JNK2 gamma 2, respectively. Among them, JNK2 alpha 4 and JNK2 gamma 2 are potential non-coding RNA because they contain pre-mature stop codons. Both JNK2 alpha 3 and JNK2 beta 3 contain an intact kinase domain, and both encode a protein product of 46 kDa, the same as those of JNK2 alpha 1 and JNK2 beta 1. JNK2 gamma 1 contains a disrupted kinase domain and it showed a disable function. When over-expressed in mammalian cells, JNK2a3 showed higher activity on AP-1 than that of JNK2 beta 3 and JNK2 gamma 1. Furthermore, JNK2 alpha 3 and JNK2 beta 3 showed different levels of substrate phosphorylation, although they both could promote the proliferation of 293T cells. Our results further demonstrate that JNK2 isoforms preferentially target different substrates and may regulate the expression of various target genes. [BMB reports 2010; 43(11): 738-743]

语种英语
所属项目编号2006AA02A305 ; 2009ZX09503-004
资助者National High Technology Research and the Development Program of China ; Key National ST Program ; National High Technology Research and the Development Program of China ; Key National ST Program
WOS记录号WOS:000284974500005
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56848
专题基础医学院_免疫学系
作者单位1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Lab Med Immunol, Beijing 100191, Peoples R China
3.Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Wang, Pingzhang,Xiong, Ying,Ma, Chuan,et al. Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1[J]. BMB REPORTS,2010,43(11):738-743.
APA Wang, Pingzhang,Xiong, Ying,Ma, Chuan,Shi, Taiping,&Ma, Dalong.(2010).Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1.BMB REPORTS,43(11),738-743.
MLA Wang, Pingzhang,et al."Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1".BMB REPORTS 43.11(2010):738-743.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
BMB043-11-05.pdf(4232KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Pingzhang]的文章
[Xiong, Ying]的文章
[Ma, Chuan]的文章
百度学术
百度学术中相似的文章
[Wang, Pingzhang]的文章
[Xiong, Ying]的文章
[Ma, Chuan]的文章
必应学术
必应学术中相似的文章
[Wang, Pingzhang]的文章
[Xiong, Ying]的文章
[Ma, Chuan]的文章
相关权益政策
暂无数据
收藏/分享
文件名: BMB043-11-05.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。