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Relationship between single nucleotide polymorphisms in the deoxycytidine kinase gene and chemosensitivity of gemcitabine in six pancreatic cancer cell lines
Si Shuang1,2; Liao Quan1,2; Zhao Yu-pei1,2; Hu Ya1,2; Zhang Qiang1,2; You Li-li3
关键词Deoxycytidine Kinase Gene Gemcitabine Single Nucleotide Polymorphism Chemosensitivity Pancreatic Neoplasm
刊名CHINESE MEDICAL JOURNAL
2011-02-05
DOI10.3760/cma.j.issn.0366-6999.2011.03.018
124期:3页:419-422
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]PHARMACOGENOMICS ; THERAPY ; IDENTIFICATION ; PHARMACOLOGY
英文摘要

Background Single nucleotide polymorphisms (SNPs) in the deoxycytidine kinase (dCK) gene are associated with chemosensitivity to nucleoside analogs. 2′,2′-Difluoro 2′-deoxycytidine (gemcitabine) is a first-line nucleoside analog drug in the treatment of pancreatic cancer. However, the association between SNPs in the dCK gene and chemosensitivity to gemcitabine has not been fully established. Therefore, the present study aimed to investigate the relationship between SNPs in the dCK gene and chemosensitivity to gemcitabine in human pancreatic cancer cell lines.

Methods Seven SNPs in the dCK gene were sequenced in six human pancreatic cancer cell lines. The chemosensitivity of these six cell lines to gemcitabine were evaluated in vitro with a Cell Counting Kit-8 (CCK-8) test. Inhibition rates were used to express the chemosensitivity of pancreatic cancer cell lines to gemcitabine.

Results The genotype of the A9846G SNP in the dCK gene was determined in six human pancreatic cancer cell lines. The cell lines BxPC-3 and T3M4 carried the A9846G SNP genotype AG, whereas cell lines AsPC-1, Mia PaCa2, SW1990 and SU86.86 carried the GG genotype. Cell lines with the AG genotype (BxPC-3 and T3M4) were more sensitive to gemcitabine compared with cell lines with the GG genotype (AsPC-1, Mia PaCa2, SW1990 and SU86.86) and significantly different inhibition rates were observed between cell lines carrying the AG and GG genotypes (P < 0.01).

Conclusions Variants in the A9846G SNP of the dCK gene were associated with sensitivity to gemcitabine in pancreatic cancer cell lines. The dCK A9846G SNP may act as a genetic marker to predict chemotherapy efficacy of gemcitabine in pancreatic cancer. Chin Med J2011;124(3):419-422

语种英语
WOS记录号WOS:000287992400018
项目编号30772526 ; 7092084
资助机构Chinese National Natural Sciences Foundation ; Beijing Natural Sciences Foundation
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56858
专题北京大学公共卫生学院
作者单位1.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Gen Surg, Natl Lab Med Mol Biol, Beijing 100730, Peoples R China
2.Peking Union Med Coll, Beijing 100730, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Publ Hlth, Beijing 100191, Peoples R China
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GB/T 7714
Si Shuang,Liao Quan,Zhao Yu-pei,et al. Relationship between single nucleotide polymorphisms in the deoxycytidine kinase gene and chemosensitivity of gemcitabine in six pancreatic cancer cell lines[J]. CHINESE MEDICAL JOURNAL,2011,124(3):419-422.
APA Si Shuang,Liao Quan,Zhao Yu-pei,Hu Ya,Zhang Qiang,&You Li-li.(2011).Relationship between single nucleotide polymorphisms in the deoxycytidine kinase gene and chemosensitivity of gemcitabine in six pancreatic cancer cell lines.CHINESE MEDICAL JOURNAL,124(3),419-422.
MLA Si Shuang,et al."Relationship between single nucleotide polymorphisms in the deoxycytidine kinase gene and chemosensitivity of gemcitabine in six pancreatic cancer cell lines".CHINESE MEDICAL JOURNAL 124.3(2011):419-422.
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