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学科主题临床医学
Exploring drug-protein interactions using the relationship between injection volume and capacity factor
Zhao, Xinfeng1; Li, Qian1; Chen, Jiejun2; Xiao, Chaoni1; Bian, Liujiao1; Zheng, Jianbin3; Zheng, Xiaohui1; Li, Zijian4,5; Zhang, Youyi4,5
关键词Drug Protein Interactions Affinity Chromatography Mathematical Model Human Serum Albumin Beta(2)-adrenoceptor
刊名JOURNAL OF CHROMATOGRAPHY A
2014-04-25
DOI10.1016/j.chroma.2014.03.017
1339页:137-144
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE AFFINITY-CHROMATOGRAPHY ; HUMAN SERUM-ALBUMIN ; STATIONARY PHASES ; FRONTAL ANALYSIS ; FLUORESCENCE SPECTROSCOPY ; CAPILLARY-ELECTROPHORESIS ; BINDING CONSTANTS ; COUPLED RECEPTOR ; RAPID ANALYSIS ; ZONAL ELUTION
英文摘要

Affinity chromatography is the most widespread and widely accepted methodology for exploring drug-protein and protein-protein interactions. Despite the successful application of frontal analysis and zonal elution in affinity chromatography, research into the creation of new mathematical tools for data processing is encouraged due to these two methods′ drawbacks of long analysis times and high ligand consumption. In this work, we created a novel mathematical model using the relationship between the molar amount of an injected solute and its capacity factor. We validated the method by analyzing the binding of drugs to human serum albumin (HSA) and beta(2)-adrenoceptor (beta(2)-AR). The association constants of omeprazole, propranolol and promethazine binding to HSA were determined to be (4.10 +/- 0.24) x 10(4), (2.30 +/- 0.12) x 10(4) and (1.24 +/- 0.14) x 10(4) M-1, respectively. These constants agreed with previously reported literature results of 4.60 x 10(4), 2.30 x 10(4) and 1.40 x 10(4) M-1. Salbutamol, norepinephrine, isoprenaline, bamethane and methoxyphenamine were found to bind to beta(2)-AR with association constants of (1.11 +/- 0.06) x 10(3), (0.95 +/- 0.03) x 10(3), (1.66 +/- 0.12) x 10(3), (0.47 +/- 0.04) x 10(3) and (0.43 +/- 0.02) x 10(3) M-1, respectively, which positively correlated to the negative logarithm of the dissociation constants obtained via radio-ligand binding assays. The proposed model is relatively fast and conserves ligand, and it has the potential to serve as an alternative method for rapidly revealing drug-protein and protein-protein interactions. (C) 2014 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000335201100017
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56868
专题北京大学第三临床医学院_心血管内科
作者单位1.NW Univ Xian, Minist Educ, Coll Life Sci, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Shaanxi, Peoples R China
2.China Natl Ctr Biotechnol Dev, Beijing 100039, Peoples R China
3.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
4.NW Univ Xian, Shaanxi Prov Key Lab Electroanalyt Chem, Inst Analyt Sci, Xian 710069, Shaanxi, Peoples R China
5.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
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Zhao, Xinfeng,Li, Qian,Chen, Jiejun,et al. Exploring drug-protein interactions using the relationship between injection volume and capacity factor[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1339:137-144.
APA Zhao, Xinfeng.,Li, Qian.,Chen, Jiejun.,Xiao, Chaoni.,Bian, Liujiao.,...&Zhang, Youyi.(2014).Exploring drug-protein interactions using the relationship between injection volume and capacity factor.JOURNAL OF CHROMATOGRAPHY A,1339,137-144.
MLA Zhao, Xinfeng,et al."Exploring drug-protein interactions using the relationship between injection volume and capacity factor".JOURNAL OF CHROMATOGRAPHY A 1339(2014):137-144.
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