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学科主题基础医学
The 5 ′-upstream region of human programmed cell death 5 gene contains a highly active TATA-less promoter that is up-regulated by etoposide
Xu, MX; Cheng, N; Gui, LM; Lai, MY; Wang, Y; Xia, DL; Rui, M; Zhang, YM; Ma, D
关键词PDCD5 5 &prime -upstream region etoposide apoptosis
刊名GENE
2004-03-31
DOI10.1016/j.gene.2003.12.025
329期:0页:39-49
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Genetics & Heredity
研究领域[WOS]Genetics & Heredity
关键词[WOS]HELA-CELLS ; KAPPA-B ; APOPTOSIS ; ACTIVATION ; EXPRESSION ; LEUKEMIA ; PROTEASE ; TFAR19 ; SITES ; DEATH
英文摘要

The PDCD5 (programmed cell death 5), a novel apoptosis related gene, is functionally associated with cell apoptosis, exhibits a ubiquitous expression pattern and is up-regulated in some types of tumor cells undergoing apoptosis. To study the transcriptional regulation of the PDCD5 gene, we have cloned 1.1 kb of its 5′-upstream region. The DNA sequencing analysis revealed a major transcriptional start site at 72 base pairs in front of the ATG translational start codon. The upstream of the transcriptional start site lacks a canonical TATA box and CAAT box. Transient transfection and luciferase assay demonstrate that this region presents extremely strong promoter activity. The 5′ deleted sequences fused to a luciferase reporter gene demonstrated that the - 555/ - 383 region from the transcription start site is crucial for transcriptional regulation, and the luciferase reporter gene′s expression significantly increased in the early stage of cell apoptosis induced by etoposide. These results imply that the PDCD5 gene may be a target gene under the control of some important apoptosis-related transcriptional factors during the cell apoptosis. (C) 2004 Elsevier B.V All rights reserved.

语种英语
WOS记录号WOS:000220856300005
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56882
专题北京大学基础医学院_免疫学系
作者单位Peking Univ, Ctr Human Dis Gen, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
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GB/T 7714
Xu, MX,Cheng, N,Gui, LM,等. The 5 ′-upstream region of human programmed cell death 5 gene contains a highly active TATA-less promoter that is up-regulated by etoposide[J]. GENE,2004,329(0):39-49.
APA Xu, MX.,Cheng, N.,Gui, LM.,Lai, MY.,Wang, Y.,...&Ma, D.(2004).The 5 ′-upstream region of human programmed cell death 5 gene contains a highly active TATA-less promoter that is up-regulated by etoposide.GENE,329(0),39-49.
MLA Xu, MX,et al."The 5 ′-upstream region of human programmed cell death 5 gene contains a highly active TATA-less promoter that is up-regulated by etoposide".GENE 329.0(2004):39-49.
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