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IR@PKUHSC  > 北京大学药学院  > 天然药物学系  > 期刊论文
学科主题: 药学
题名:
Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria
作者: Wang, Cheng-Zhi2; Ma, Xiao-Qing1; Yang, Dong-Hui1; Guo, Zhi-Rong2; Liu, Gui-Rong3,5; Zhao, Ge-Xin2; Tang, Jie2; Zhang, Ya-Nan2; Ma, Miao1; Cai, Shao-Qing1; Ku, Bao-Shan4; Liu, Shu-Lin2,3,5
刊名: BMC MICROBIOLOGY
发表日期: 2010-04-16
DOI: 10.1186/1471-2180-10-115
卷: 10
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: SECOISOLARICIRESINOL DIGLUCOSIDE ; MAMMALIAN LIGNANS ; SALMONELLA-TYPHIMURIUM ; ENTEROLACTONE ; METABOLISM ; GENOME ; PHYTOESTROGENS ; TRANSFORMATION ; PRECURSORS
英文摘要:

Background: The effects of enterolignans, e. g., enterodiol (END) and particularly its oxidation product, enterolactone (ENL), on prevention of hormone-dependent diseases, such as osteoporosis, cardiovascular diseases, hyperlipemia, breast cancer, colon cancer, prostate cancer and menopausal syndrome, have attracted much attention. To date, the main way to obtain END and ENL is chemical synthesis, which is expensive and inevitably leads to environmental pollution. To explore a more economic and eco-friendly production method, we explored biotransformation of enterolignans from precursors contained in defatted flaxseeds by human intestinal bacteria.

Results: We cultured fecal specimens from healthy young adults in media containing defatted flaxseeds and detected END from the culture supernatant. Following selection through successive subcultures of the fecal microbiota with defatted flaxseeds as the only carbon source, we obtained a bacterial consortium, designated as END-49, which contained the smallest number of bacterial types still capable of metabolizing defatted flaxseeds to produce END. Based on analysis with pulsed field gel electrophoresis, END-49 was found to consist of five genomically distinct bacterial lineages, designated Group I-V, with Group I strains dominating the culture. None of the individual Group I-V strains produced END, demonstrating that the biotransformation of substrates in defatted flaxseeds into END is a joint work by different members of the END-49 bacterial consortium. Interestingly, Group I strains produced secoisolariciresinol, an important intermediate of END production; 16S rRNA analysis of one Group I strain established its close relatedness with Klebsiella. Genomic analysis is under way to identify all members in END-49 involved in the biotransformation and the actual pathway leading to END-production.

Conclusion: Biotransformation is a very economic, efficient and environmentally friendly way of mass-producing enterodiol from defatted flaxseeds.

语种: 英语
所属项目编号: 30672622 ; 30370774 ; 30870098 ; 30970119
项目资助者: National Natural Science Foundation of China ; Peking University Health Science Center
WOS记录号: WOS:000277522300001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56891
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100191, Peoples R China
2.Peking Univ, Dept Microbiol, Hlth Sci Ctr, Beijing 100191, Peoples R China
3.Harbin Med Coll, Genom Res Ctr, Harbin 150081, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Beijing 100191, Peoples R China
5.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB T2N 4N1, Canada

Recommended Citation:
Wang, Cheng-Zhi,Ma, Xiao-Qing,Yang, Dong-Hui,et al. Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria[J]. BMC MICROBIOLOGY,2010,10.
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