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The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3T3 cells
Li, MS; Li, PF; Yang, FY; He, SP; Du, GG; Li, G
关键词Alpha-fetoprotein Receptor Signal Transduction Monoclonal Antibody Gene Expression
刊名CELL RESEARCH
2002-06-01
12期:2页:151-156
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]GENE-EXPRESSION ; P21 EXPRESSION ; GROWTH-FACTOR ; TUMOR ; P53 ; RECEPTOR ; PROTEIN
英文摘要

AIM The existence and properties of alpha-fetoprotein (AFP) receptor on the surface of NIH 3T3 cells and the effects of AFP on cellular signal transduction pathway were investigated. METHODS The effect of AFP on the proliferation of NIH 3T3 cells was measured by incorporation of H-3-TdR. Receptor-binding assay of I-125-AFP was performed to detect the properties of AFP receptor in NIH 3T3 cells. The influences of AFP on the [cAMP]i and the activities of protein kinase A (PKA) were determined. Western blot was used to detect the change of K-ras P21 protein expression. RESULTS The proliferation of NIH 3T3 cells treated with 0-80 mg/L of AFP was significantly enhanced. The Scatchard analysis indicated that there were two classes of binding sites with K-D of 2.722x10(-9)M (Bmax=12810 sites per cell) and 8.931x10(-8)M (Bmax=119700 sites per cell) respectively. In the presence of AFP (20 mg/L), the content of cAMP and activities of PKA were significantly elevated. The level of K-ras P21 protein was upregulated by AFP at the concentration of 20 mg/L. The monoclonal antibody against AFP could reverse the effects of AFP on the cAMP content, PKA activity and the expression of K-ras p21 gene. CONCLUSION The effect of AFP on the cell proliferation was achieved by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 gene.

语种英语
WOS记录号WOS:000176627300008
引用统计
被引频次:53[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56904
专题基础医学院_生物物理学系
作者单位1.Peking Univ, Dept Biochem & Mol Biol, Hlth Sci Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Dept Biophys, Hlth Sci Ctr, Beijing 100083, Peoples R China
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GB/T 7714
Li, MS,Li, PF,Yang, FY,et al. The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3T3 cells[J]. CELL RESEARCH,2002,12(2):151-156.
APA Li, MS,Li, PF,Yang, FY,He, SP,Du, GG,&Li, G.(2002).The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3T3 cells.CELL RESEARCH,12(2),151-156.
MLA Li, MS,et al."The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3T3 cells".CELL RESEARCH 12.2(2002):151-156.
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