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学科主题: 基础医学
题名:
The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3T3 cells
作者: Li, MS; Li, PF; Yang, FY; He, SP; Du, GG; Li, G
关键词: alpha-fetoprotein ; receptor ; signal transduction ; monoclonal antibody ; gene expression
刊名: CELL RESEARCH
发表日期: 2002-06-01
卷: 12, 期:2, 页:151-156
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: GENE-EXPRESSION ; P21 EXPRESSION ; GROWTH-FACTOR ; TUMOR ; P53 ; RECEPTOR ; PROTEIN
英文摘要:

AIM The existence and properties of alpha-fetoprotein (AFP) receptor on the surface of NIH 3T3 cells and the effects of AFP on cellular signal transduction pathway were investigated. METHODS The effect of AFP on the proliferation of NIH 3T3 cells was measured by incorporation of H-3-TdR. Receptor-binding assay of I-125-AFP was performed to detect the properties of AFP receptor in NIH 3T3 cells. The influences of AFP on the [cAMP]i and the activities of protein kinase A (PKA) were determined. Western blot was used to detect the change of K-ras P21 protein expression. RESULTS The proliferation of NIH 3T3 cells treated with 0-80 mg/L of AFP was significantly enhanced. The Scatchard analysis indicated that there were two classes of binding sites with K-D of 2.722x10(-9)M (Bmax=12810 sites per cell) and 8.931x10(-8)M (Bmax=119700 sites per cell) respectively. In the presence of AFP (20 mg/L), the content of cAMP and activities of PKA were significantly elevated. The level of K-ras P21 protein was upregulated by AFP at the concentration of 20 mg/L. The monoclonal antibody against AFP could reverse the effects of AFP on the cAMP content, PKA activity and the expression of K-ras p21 gene. CONCLUSION The effect of AFP on the cell proliferation was achieved by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 gene.

语种: 英语
WOS记录号: WOS:000176627300008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56904
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Dept Biochem & Mol Biol, Hlth Sci Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Dept Biophys, Hlth Sci Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Li, MS,Li, PF,Yang, FY,et al. The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3T3 cells[J]. CELL RESEARCH,2002,12(2):151-156.
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